@inbook{b40a7cea04fd41f0afd5b1c4afc87fdd,
title = "Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-Free Fractions, and Cells",
abstract = "Cellular Communication Network (CCN) proteins are growth factors that play key roles in many pathophysiological events, including bone formation, wound healing, and cancer. CCN factors and fragments generated by metalloproteinases-dependent cleavage are often associated with extracellular matrix (ECM) or small extracellular vesicles (sEVs) such as exosomes or matrix-coated vesicles. We provide reliable methods and protocols for Western blotting to analyze CCN factors and fragments in cells, sEVs, and vesicle-free fractions.",
keywords = "CCN family proteins, CCN fragments, Cell communication network, CTGF, Exosomes, Extracellular matrix, Extracellular vesicles, Matricellular proteins, Matrix vesicles, Metalloproteinases",
author = "Kisho Ono and Yuka Okusya and Tran, {Manh Tien} and Koki Umemori and Takanori Eguchi",
note = "Funding Information: T.E. was supported by JSPS Kakenhi, grant numbers 17K11642-TE, 20K09904-CS, 19H03817-MT, 20H03888-HN, 20K20611-MT, 20H03888-HN, 21H03119-TY, and 21K08902-HY. Y.O. was supported by JSPS overseas research fellowship. K.O. was supported by JSPS Kakenhi, grant number 21K17115. The authors thank Eriko Aoyama, Shiro Fukuoka, Satoshi Kubota, Kuniaki Okamoto, Chiharu Sogawa, Eman Taha, and Masaharu Takigawa for useful information, discussion, materials, or experimentation. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2744-0_5",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "39--57",
booktitle = "Methods in Molecular Biology",
}
