Wax ester production from n-alkanes by Acinetobacter sp. strain M-1: Ultrastructure of cellular inclusions and role of acyl coenzyme a reductase

Takeru Ishige, Akiwo Tani, Keiji Takabe, Kazunori Kawasaki, Yasuyoshi Sakai, Nobuo Kato

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Acinetobacter sp. strain M-1 accumulated a large amount of wax esters from an n-alkane under nitrogen-limiting conditions. Under the optimized conditions with n-hexadecane as the substrate, the amount of hexadecyl hexadecanoate in the cells reached 0.17 g/g of cells (dry weight). Electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant with wax ester formation. The contribution of acyl-CoA reductase to wax ester synthesis was evaluated by gene disruption analysis.

Original languageEnglish
Pages (from-to)1192-1195
Number of pages4
JournalApplied and Environmental Microbiology
Volume68
Issue number3
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

wax esters
Acinetobacter
Alkanes
coenzymes
Waxes
Inclusion Bodies
Coenzymes
ultrastructure
wax
alkane
alkanes
ester
Oxidoreductases
Esters
hexadecane
Palmitates
gene targeting
Nitrogen
electrons
cells

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Wax ester production from n-alkanes by Acinetobacter sp. strain M-1 : Ultrastructure of cellular inclusions and role of acyl coenzyme a reductase. / Ishige, Takeru; Tani, Akiwo; Takabe, Keiji; Kawasaki, Kazunori; Sakai, Yasuyoshi; Kato, Nobuo.

In: Applied and Environmental Microbiology, Vol. 68, No. 3, 2002, p. 1192-1195.

Research output: Contribution to journalArticle

Ishige, Takeru ; Tani, Akiwo ; Takabe, Keiji ; Kawasaki, Kazunori ; Sakai, Yasuyoshi ; Kato, Nobuo. / Wax ester production from n-alkanes by Acinetobacter sp. strain M-1 : Ultrastructure of cellular inclusions and role of acyl coenzyme a reductase. In: Applied and Environmental Microbiology. 2002 ; Vol. 68, No. 3. pp. 1192-1195.
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