Water-soluble complexes formed by natural polyphenols and bovine serum albumin: Evidence from gel electrophoresis

Miwako Kusuda, Tsutomu Hatano, Takashi Yoshida

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The formation of water-soluble complexes from combinations of various polyphenols and bovine serum albumin (BSA) was analyzed by gel electrophoresis. Sodium dodecylsulfate (SDS) and native polyacrylamide gel electrophoretic (PAGE) analyses clearly showed complexes formed from BSA with oenothein B, corilagin, (+)-catechin, procyanidin B3, and gallic acid derivatives; however, it was difficult to distinguish between the complexes containing a single BSA molecule and the BSA molecule itself by using size-exclusion chromatography (SEC). Combining SDS and native PAGE analyses showed instability in the macromolecular complexes formed from pentagalloylglucose (PGG) and BSA. Research also revealed that the oxidation of (-)-epigallocatechin gallate (EGCG) contributed to the formation of more stable macromolecular complexes from EGCG and BSA.

Original languageEnglish
Pages (from-to)152-160
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number1
DOIs
Publication statusPublished - 2006

Keywords

  • Bovine serum albumin
  • Catechin
  • Complex
  • Polyphenol
  • Sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis (PAGE)

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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