Walnut polyphenols prevent liver damage induced by carbon tetrachloride and D-galactosamine: Hepatoprotective hydrolyzable tannins in the kernel pellicles of walnut

Hiroshi Shimoda, Junji Tanaka, Mitsunori Kikuchi, Toshiyuji Fukuda, Hideyuki Ito, Tsutomu Hatano, Takashi Yoshida

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58 Citations (Scopus)


The polyphenol-rich fraction (WP, 45% polyphenol) prepared from the kernel pellicles of walnuts was assessed for its hepatoprotective effect in mice. A single oral administration of WP (200 mg/kg) significantly suppressed serum glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) elevation in liver injury induced by carbon tetrachloride (CCl4), while it did not suppress D-galactosamine (GalN)-induced liver injury. In order to identify the active principles in WP, we examined individual constituents for the protective effect on cell damage induced by CCl4 and D-GalN in primary cultured rat hepatocytes. WP was effective against both CCl4- and D-GalN-induced hepatocyte damages. Among the constituents, only ellagitannins with a galloylated glucopyranose core, such as tellimagrandins I, II, and rugosin C, suppressed CCl4-induced hepatocyte damage significantly. Most of the ellagitannins including tellimagrandin I and 2,3-O-hexahydroxydiphenoyl-glucose exhibited remarkable inhibitory effect against D-GalN-induced damage. Telliamgrandin I especially completely suppressed both CCl4- and D-GalN-induced cell damage, and thus is likely the principal constituent for the hepatoprotective effect of WP.

Original languageEnglish
Pages (from-to)4444-4449
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Issue number12
Publication statusPublished - Jun 25 2008



  • Carbon tetrachloride
  • D-galactosamine
  • Hydrolyzable tannin
  • Liver injury
  • Polyphenol
  • Tellimagrandin I
  • Walnut

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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