Wall shear modulation of cytokines in early vein grafts

Zhihua Jiang, Scott A. Berceli, Chun L. Pfahnl, Lizhen Wu, Darin Goldman, Ming Tao, Motoko Kagayama, Akihiro Matsukawa, C. Keith Ozaki

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Objective Pro-inflammatory cytokine-driven mechanisms have been implicated in vein graft failure, though little is known about the effect of hemodynamic factors and anti-inflammatory counter-regulatory mechanisms. We hypothesized that early temporal expression of the pro-inflammatory cytokine interleukin (IL)-1β and the anti-inflammatory cytokine IL-10 proceeds by way of wall shear stress-dependent pathways in the arterializing vein graft. Methods Rabbits (n = 27) underwent bilateral jugular vein carotid interposition grafts, and simultaneous unilateral distal carotid branch ligation, to produce both low-flow and high-flow grafts in the same animal. Vein grafts were harvested at 1, 3, 7, 14, and 28 days and were assessed for architecture, wall shear stress, and cytokine messenger RNA levels (quantitative real-time two-step reverse transcription polymerase chain reaction). Results The model resulted in an immediate 90% flow reduction (P <.001, paired t test) in the vein graft on the ligated side, and a 36% increase (P = .01) in contralateral graft flow. This persisted as ∼15-fold flow differential throughout the 28-day period. The construction yielded a 15-fold differential in wall shear stress between low-flow and high-flow vein grafts (P <.001, two-way repeated measures analysis of variance). Intimal hyperplasia began by day 3, and was 6-fold more in low wall shear grafts by 28 days (230.6 ± 35.4 μm intimal thickness vs 36.1 ± 17.6 μm for low shear versus high shear grafts; P = .001). For both cytokines time independently affected mRNA expression (P <.001, global analysis of variance). Exposure of vein grafts to the arterial circulation markedly up-regulated IL-1β at 1 day, with significantly more induction in the low shear setting (P = .002). IL-1β protein localized to the developing neointima at days 1 and 3. Conversely, IL-10 slowly increased until day 14, with significantly more expression in the high shear grafts (P <.001). Conclusions Vein graft adaptation induces early pro-inflammatory cytokine IL-1β expression and delayed protective IL-10 expression (most notable under high shear conditions), both of which are modulated by wall shear. These differential temporal windows offer strategies for appropriately timed pro-inflammatory or anti-inflammatory therapies to interrupt pathologic vein graft adaptations.

Original languageEnglish
Pages (from-to)345-350
Number of pages6
JournalJournal of Vascular Surgery
Volume40
Issue number2
DOIs
Publication statusPublished - Aug 2004
Externally publishedYes

Fingerprint

Veins
Cytokines
Transplants
Interleukin-1
Interleukin-10
Tunica Intima
Anti-Inflammatory Agents
Analysis of Variance
Neointima
Messenger RNA
Jugular Veins
Reverse Transcription
Hyperplasia
Ligation
Hemodynamics
Rabbits
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Jiang, Z., Berceli, S. A., Pfahnl, C. L., Wu, L., Goldman, D., Tao, M., ... Ozaki, C. K. (2004). Wall shear modulation of cytokines in early vein grafts. Journal of Vascular Surgery, 40(2), 345-350. https://doi.org/10.1016/j.jvs.2004.03.048

Wall shear modulation of cytokines in early vein grafts. / Jiang, Zhihua; Berceli, Scott A.; Pfahnl, Chun L.; Wu, Lizhen; Goldman, Darin; Tao, Ming; Kagayama, Motoko; Matsukawa, Akihiro; Ozaki, C. Keith.

In: Journal of Vascular Surgery, Vol. 40, No. 2, 08.2004, p. 345-350.

Research output: Contribution to journalArticle

Jiang, Z, Berceli, SA, Pfahnl, CL, Wu, L, Goldman, D, Tao, M, Kagayama, M, Matsukawa, A & Ozaki, CK 2004, 'Wall shear modulation of cytokines in early vein grafts', Journal of Vascular Surgery, vol. 40, no. 2, pp. 345-350. https://doi.org/10.1016/j.jvs.2004.03.048
Jiang Z, Berceli SA, Pfahnl CL, Wu L, Goldman D, Tao M et al. Wall shear modulation of cytokines in early vein grafts. Journal of Vascular Surgery. 2004 Aug;40(2):345-350. https://doi.org/10.1016/j.jvs.2004.03.048
Jiang, Zhihua ; Berceli, Scott A. ; Pfahnl, Chun L. ; Wu, Lizhen ; Goldman, Darin ; Tao, Ming ; Kagayama, Motoko ; Matsukawa, Akihiro ; Ozaki, C. Keith. / Wall shear modulation of cytokines in early vein grafts. In: Journal of Vascular Surgery. 2004 ; Vol. 40, No. 2. pp. 345-350.
@article{6a65a5f90cc54e32b5dc76d745468c8d,
title = "Wall shear modulation of cytokines in early vein grafts",
abstract = "Objective Pro-inflammatory cytokine-driven mechanisms have been implicated in vein graft failure, though little is known about the effect of hemodynamic factors and anti-inflammatory counter-regulatory mechanisms. We hypothesized that early temporal expression of the pro-inflammatory cytokine interleukin (IL)-1β and the anti-inflammatory cytokine IL-10 proceeds by way of wall shear stress-dependent pathways in the arterializing vein graft. Methods Rabbits (n = 27) underwent bilateral jugular vein carotid interposition grafts, and simultaneous unilateral distal carotid branch ligation, to produce both low-flow and high-flow grafts in the same animal. Vein grafts were harvested at 1, 3, 7, 14, and 28 days and were assessed for architecture, wall shear stress, and cytokine messenger RNA levels (quantitative real-time two-step reverse transcription polymerase chain reaction). Results The model resulted in an immediate 90{\%} flow reduction (P <.001, paired t test) in the vein graft on the ligated side, and a 36{\%} increase (P = .01) in contralateral graft flow. This persisted as ∼15-fold flow differential throughout the 28-day period. The construction yielded a 15-fold differential in wall shear stress between low-flow and high-flow vein grafts (P <.001, two-way repeated measures analysis of variance). Intimal hyperplasia began by day 3, and was 6-fold more in low wall shear grafts by 28 days (230.6 ± 35.4 μm intimal thickness vs 36.1 ± 17.6 μm for low shear versus high shear grafts; P = .001). For both cytokines time independently affected mRNA expression (P <.001, global analysis of variance). Exposure of vein grafts to the arterial circulation markedly up-regulated IL-1β at 1 day, with significantly more induction in the low shear setting (P = .002). IL-1β protein localized to the developing neointima at days 1 and 3. Conversely, IL-10 slowly increased until day 14, with significantly more expression in the high shear grafts (P <.001). Conclusions Vein graft adaptation induces early pro-inflammatory cytokine IL-1β expression and delayed protective IL-10 expression (most notable under high shear conditions), both of which are modulated by wall shear. These differential temporal windows offer strategies for appropriately timed pro-inflammatory or anti-inflammatory therapies to interrupt pathologic vein graft adaptations.",
author = "Zhihua Jiang and Berceli, {Scott A.} and Pfahnl, {Chun L.} and Lizhen Wu and Darin Goldman and Ming Tao and Motoko Kagayama and Akihiro Matsukawa and Ozaki, {C. Keith}",
year = "2004",
month = "8",
doi = "10.1016/j.jvs.2004.03.048",
language = "English",
volume = "40",
pages = "345--350",
journal = "Journal of Vascular Surgery",
issn = "0741-5214",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - Wall shear modulation of cytokines in early vein grafts

AU - Jiang, Zhihua

AU - Berceli, Scott A.

AU - Pfahnl, Chun L.

AU - Wu, Lizhen

AU - Goldman, Darin

AU - Tao, Ming

AU - Kagayama, Motoko

AU - Matsukawa, Akihiro

AU - Ozaki, C. Keith

PY - 2004/8

Y1 - 2004/8

N2 - Objective Pro-inflammatory cytokine-driven mechanisms have been implicated in vein graft failure, though little is known about the effect of hemodynamic factors and anti-inflammatory counter-regulatory mechanisms. We hypothesized that early temporal expression of the pro-inflammatory cytokine interleukin (IL)-1β and the anti-inflammatory cytokine IL-10 proceeds by way of wall shear stress-dependent pathways in the arterializing vein graft. Methods Rabbits (n = 27) underwent bilateral jugular vein carotid interposition grafts, and simultaneous unilateral distal carotid branch ligation, to produce both low-flow and high-flow grafts in the same animal. Vein grafts were harvested at 1, 3, 7, 14, and 28 days and were assessed for architecture, wall shear stress, and cytokine messenger RNA levels (quantitative real-time two-step reverse transcription polymerase chain reaction). Results The model resulted in an immediate 90% flow reduction (P <.001, paired t test) in the vein graft on the ligated side, and a 36% increase (P = .01) in contralateral graft flow. This persisted as ∼15-fold flow differential throughout the 28-day period. The construction yielded a 15-fold differential in wall shear stress between low-flow and high-flow vein grafts (P <.001, two-way repeated measures analysis of variance). Intimal hyperplasia began by day 3, and was 6-fold more in low wall shear grafts by 28 days (230.6 ± 35.4 μm intimal thickness vs 36.1 ± 17.6 μm for low shear versus high shear grafts; P = .001). For both cytokines time independently affected mRNA expression (P <.001, global analysis of variance). Exposure of vein grafts to the arterial circulation markedly up-regulated IL-1β at 1 day, with significantly more induction in the low shear setting (P = .002). IL-1β protein localized to the developing neointima at days 1 and 3. Conversely, IL-10 slowly increased until day 14, with significantly more expression in the high shear grafts (P <.001). Conclusions Vein graft adaptation induces early pro-inflammatory cytokine IL-1β expression and delayed protective IL-10 expression (most notable under high shear conditions), both of which are modulated by wall shear. These differential temporal windows offer strategies for appropriately timed pro-inflammatory or anti-inflammatory therapies to interrupt pathologic vein graft adaptations.

AB - Objective Pro-inflammatory cytokine-driven mechanisms have been implicated in vein graft failure, though little is known about the effect of hemodynamic factors and anti-inflammatory counter-regulatory mechanisms. We hypothesized that early temporal expression of the pro-inflammatory cytokine interleukin (IL)-1β and the anti-inflammatory cytokine IL-10 proceeds by way of wall shear stress-dependent pathways in the arterializing vein graft. Methods Rabbits (n = 27) underwent bilateral jugular vein carotid interposition grafts, and simultaneous unilateral distal carotid branch ligation, to produce both low-flow and high-flow grafts in the same animal. Vein grafts were harvested at 1, 3, 7, 14, and 28 days and were assessed for architecture, wall shear stress, and cytokine messenger RNA levels (quantitative real-time two-step reverse transcription polymerase chain reaction). Results The model resulted in an immediate 90% flow reduction (P <.001, paired t test) in the vein graft on the ligated side, and a 36% increase (P = .01) in contralateral graft flow. This persisted as ∼15-fold flow differential throughout the 28-day period. The construction yielded a 15-fold differential in wall shear stress between low-flow and high-flow vein grafts (P <.001, two-way repeated measures analysis of variance). Intimal hyperplasia began by day 3, and was 6-fold more in low wall shear grafts by 28 days (230.6 ± 35.4 μm intimal thickness vs 36.1 ± 17.6 μm for low shear versus high shear grafts; P = .001). For both cytokines time independently affected mRNA expression (P <.001, global analysis of variance). Exposure of vein grafts to the arterial circulation markedly up-regulated IL-1β at 1 day, with significantly more induction in the low shear setting (P = .002). IL-1β protein localized to the developing neointima at days 1 and 3. Conversely, IL-10 slowly increased until day 14, with significantly more expression in the high shear grafts (P <.001). Conclusions Vein graft adaptation induces early pro-inflammatory cytokine IL-1β expression and delayed protective IL-10 expression (most notable under high shear conditions), both of which are modulated by wall shear. These differential temporal windows offer strategies for appropriately timed pro-inflammatory or anti-inflammatory therapies to interrupt pathologic vein graft adaptations.

UR - http://www.scopus.com/inward/record.url?scp=3442881585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3442881585&partnerID=8YFLogxK

U2 - 10.1016/j.jvs.2004.03.048

DO - 10.1016/j.jvs.2004.03.048

M3 - Article

C2 - 15297832

AN - SCOPUS:3442881585

VL - 40

SP - 345

EP - 350

JO - Journal of Vascular Surgery

JF - Journal of Vascular Surgery

SN - 0741-5214

IS - 2

ER -