Vulnerability to cavitation differs between current-year and older xylem: Non-destructive observation with a compact magnetic resonance imaging system of two deciduous diffuse-porous species

Kenji Fukuda, Daichi Kawaguchi, Tomo Aihara, Mayumi Y. Ogasa, Naoko H. Miki, Tomoyuki Haishi, Toshihiro Umebayashi

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20 Citations (Scopus)

Abstract

Development of xylem embolism during water stress in two diffuse-porous hardwoods, Katsura (Cercidiphyllum japonicum) and Japanese white birch (Betula platyphylla var. japonica), was observed non-destructively under a compact magnetic resonance imaging (MRI) system in addition to conventional quantitation of hydraulic vulnerability to cavitation from excised stem segments. Distribution of white and dark areas in MR images corresponded well to the distribution of water-filled/embolized vessels observed by cryo-scanning electron microscopy in both species. Water-filled vessels were observed in MR images as white areas in Katsura and as white dots in Japanese white birch, respectively, and embolisms could be detected as a change to dark areas. The increase in the relative embolized area (REA: %) in the cross-sectional area of total xylem during water stress, which was estimated from the binarized MR images, was consistent with the hydraulic vulnerability curves of these species. From the non-destructive MRI observations, cavitation induced by water stress was shown to develop earlier in 1- or 2-year-old xylem than in the current-year xylem in both species; that is, the vulnerability to cavitation differs between vessels in the current-year xylem and those in older annual rings. Development of xylem embolism during water stress in two diffuse-porous hardwoods, Katsura (Cercidiphyllum japonicum) and Japanese white birch (Betula platyphylla var. japonica), was observed nondestructively under a compact magnetic resonance imaging (MRI) system in addition to conventional quantitation of hydraulic vulnerability to cavitation from excised stem segments. The increase in the relative embolized area estimated from the binarized MR images was consistent with the hydraulic vulnerability curves of these species. From the MRI observations, cavitation induced by water stress was shown to develop earlier in 1- or 2-year-old xylem than in the current-year xylem in the both species; i.e., the vulnerability to cavitation differs between vessels in the current-year xylem and those in older annual rings.

Original languageEnglish
Pages (from-to)2508-2518
Number of pages11
JournalPlant Cell and Environment
Volume38
Issue number12
DOIs
Publication statusPublished - Dec 1 2015

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Keywords

  • Annual ring
  • Drought
  • Embolism
  • MRI
  • Water relations
  • Xylem transport

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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