Vrn-D4 is a vernalization gene located on the centromeric region of chromosome 5D in hexaploid wheat

Tetsuya Yoshida, Hidetaka Nishida, Jie Zhu, Rebecca Nitcher, Assaf Distelfeld, Yukari Akashi, Kenji Kato, Jorge Dubcovsky

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Abstract

Natural variation in wheat requirement of long exposures to cold temperatures to accelerate flowering (vernalization) is mainly controlled by the Vrn-1, Vrn-2, Vrn-3, and Vrn-4 loci. The first three loci have been well characterized, but limited information is available for Vrn-4. So far, natural variation for Vrn-4 has been detected only in the D genome (Vrn-D4), and genetic stocks for this gene are available in Triple Dirk (TDF, hereafter). We detected heterogeneity in the Vrn-1 alleles present in different TDF stocks, which may explain inconsistencies among previous studies. A correct TDF seed stock from Japan carrying recessive vrn-A1, vrn-B1, and vrn-D1 alleles was crossed with three different winter cultivars to generate F2 mapping populations. Most of the variation in flowering time in these three populations was controlled by a single locus, Vrn-D4, which was mapped within a 1.8cM interval flanked by markers Xcfd78 and Xbarc205 in the centromeric region of chromosome 5D. A factorial ANOVA for heading time using Vrn-D4 alleles and vernalization as factors showed a significant interaction (P

Original languageEnglish
Pages (from-to)543-552
Number of pages10
JournalTheoretical And Applied Genetics
Volume120
Issue number3
DOIs
Publication statusPublished - Jan 2010

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vernalization
hexaploidy
Triticum
Chromosomes
Alleles
alleles
chromosomes
loci
wheat
Genes
flowering
genes
heading
Population
Seeds
Analysis of Variance
Japan
analysis of variance
Genome
genome

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Biotechnology

Cite this

Vrn-D4 is a vernalization gene located on the centromeric region of chromosome 5D in hexaploid wheat. / Yoshida, Tetsuya; Nishida, Hidetaka; Zhu, Jie; Nitcher, Rebecca; Distelfeld, Assaf; Akashi, Yukari; Kato, Kenji; Dubcovsky, Jorge.

In: Theoretical And Applied Genetics, Vol. 120, No. 3, 01.2010, p. 543-552.

Research output: Contribution to journalArticle

Yoshida, Tetsuya ; Nishida, Hidetaka ; Zhu, Jie ; Nitcher, Rebecca ; Distelfeld, Assaf ; Akashi, Yukari ; Kato, Kenji ; Dubcovsky, Jorge. / Vrn-D4 is a vernalization gene located on the centromeric region of chromosome 5D in hexaploid wheat. In: Theoretical And Applied Genetics. 2010 ; Vol. 120, No. 3. pp. 543-552.
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