Abstract
Natural variation in wheat requirement of long exposures to cold temperatures to accelerate flowering (vernalization) is mainly controlled by the Vrn-1, Vrn-2, Vrn-3, and Vrn-4 loci. The first three loci have been well characterized, but limited information is available for Vrn-4. So far, natural variation for Vrn-4 has been detected only in the D genome (Vrn-D4), and genetic stocks for this gene are available in Triple Dirk (TDF, hereafter). We detected heterogeneity in the Vrn-1 alleles present in different TDF stocks, which may explain inconsistencies among previous studies. A correct TDF seed stock from Japan carrying recessive vrn-A1, vrn-B1, and vrn-D1 alleles was crossed with three different winter cultivars to generate F2 mapping populations. Most of the variation in flowering time in these three populations was controlled by a single locus, Vrn-D4, which was mapped within a 1.8cM interval flanked by markers Xcfd78 and Xbarc205 in the centromeric region of chromosome 5D. A factorial ANOVA for heading time using Vrn-D4 alleles and vernalization as factors showed a significant interaction (P
| Original language | English |
|---|---|
| Pages (from-to) | 543-552 |
| Number of pages | 10 |
| Journal | Theoretical And Applied Genetics |
| Volume | 120 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 2010 |
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ASJC Scopus subject areas
- Agronomy and Crop Science
- Genetics
- Biotechnology
Cite this
Vrn-D4 is a vernalization gene located on the centromeric region of chromosome 5D in hexaploid wheat. / Yoshida, Tetsuya; Nishida, Hidetaka; Zhu, Jie; Nitcher, Rebecca; Distelfeld, Assaf; Akashi, Yukari; Kato, Kenji; Dubcovsky, Jorge.
In: Theoretical And Applied Genetics, Vol. 120, No. 3, 01.2010, p. 543-552.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Vrn-D4 is a vernalization gene located on the centromeric region of chromosome 5D in hexaploid wheat
AU - Yoshida, Tetsuya
AU - Nishida, Hidetaka
AU - Zhu, Jie
AU - Nitcher, Rebecca
AU - Distelfeld, Assaf
AU - Akashi, Yukari
AU - Kato, Kenji
AU - Dubcovsky, Jorge
PY - 2010/1
Y1 - 2010/1
N2 - Natural variation in wheat requirement of long exposures to cold temperatures to accelerate flowering (vernalization) is mainly controlled by the Vrn-1, Vrn-2, Vrn-3, and Vrn-4 loci. The first three loci have been well characterized, but limited information is available for Vrn-4. So far, natural variation for Vrn-4 has been detected only in the D genome (Vrn-D4), and genetic stocks for this gene are available in Triple Dirk (TDF, hereafter). We detected heterogeneity in the Vrn-1 alleles present in different TDF stocks, which may explain inconsistencies among previous studies. A correct TDF seed stock from Japan carrying recessive vrn-A1, vrn-B1, and vrn-D1 alleles was crossed with three different winter cultivars to generate F2 mapping populations. Most of the variation in flowering time in these three populations was controlled by a single locus, Vrn-D4, which was mapped within a 1.8cM interval flanked by markers Xcfd78 and Xbarc205 in the centromeric region of chromosome 5D. A factorial ANOVA for heading time using Vrn-D4 alleles and vernalization as factors showed a significant interaction (P
AB - Natural variation in wheat requirement of long exposures to cold temperatures to accelerate flowering (vernalization) is mainly controlled by the Vrn-1, Vrn-2, Vrn-3, and Vrn-4 loci. The first three loci have been well characterized, but limited information is available for Vrn-4. So far, natural variation for Vrn-4 has been detected only in the D genome (Vrn-D4), and genetic stocks for this gene are available in Triple Dirk (TDF, hereafter). We detected heterogeneity in the Vrn-1 alleles present in different TDF stocks, which may explain inconsistencies among previous studies. A correct TDF seed stock from Japan carrying recessive vrn-A1, vrn-B1, and vrn-D1 alleles was crossed with three different winter cultivars to generate F2 mapping populations. Most of the variation in flowering time in these three populations was controlled by a single locus, Vrn-D4, which was mapped within a 1.8cM interval flanked by markers Xcfd78 and Xbarc205 in the centromeric region of chromosome 5D. A factorial ANOVA for heading time using Vrn-D4 alleles and vernalization as factors showed a significant interaction (P
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U2 - 10.1007/s00122-009-1174-3
DO - 10.1007/s00122-009-1174-3
M3 - Article
C2 - 19847391
AN - SCOPUS:75849155906
VL - 120
SP - 543
EP - 552
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
SN - 0040-5752
IS - 3
ER -