TY - JOUR
T1 - VR1-, VRL-1- and P2X3 receptor-immunoreactive innervation of the rat temporomandibular joint
AU - Ichikawa, H.
AU - Fukunaga, T.
AU - Jin, H. W.
AU - Fujita, M.
AU - Takano-Yamamoto, T.
AU - Sugimoto, T.
N1 - Funding Information:
We greatly appreciate Dr. David Julius at the Department of Cellular and Molecular Pharmacology, University of California for his kind gift of the anti-VRL-1-serum. This work was supported by a grant from the Japanese Ministry of Education, Culture, Sports, Science and Technology to H.I. (No. 14571733).
PY - 2004/5/15
Y1 - 2004/5/15
N2 - Immunohistochemistry for vanilloid receptor subtype 1 (VR1), vanilloid receptor 1-like receptor (VRL-1) and P2X3 receptor was performed in the rat temporomandibular joint (TMJ). Blood vessels in the articular disk and capsule, the synovial membrane and the fibrous tissue around the condylar process were innervated by VR1- or P2X3 receptor-immunoreactive (ir) nerve fibers. However, VRL-1-immunoreactivity (ir) could not be detected in the TMJ. Retrograde tracing and immunohistochemical methods revealed that 25%, 41% and 52% of TMJ neurons in the trigeminal ganglion (TG) exhibited VR1-, VRL-1- and P2X3 receptor-ir, respectively. VR1-ir TMJ neurons were mostly small to medium-sized, whereas VRL-1- and P2X3 receptor-ir TMJ neurons were predominantly medium-sized to large. In addition, 73%, 28% and 44% of VR1-, VRL-1- and P2X3 receptor-ir TMJ neurons, respectively, coexpressed calcitonin gene-related peptide (CGRP)-ir. The present study suggests that the TMJ has abundant nociceptors which respond to vanilloid compounds, protons, heat and extracellular ATP.
AB - Immunohistochemistry for vanilloid receptor subtype 1 (VR1), vanilloid receptor 1-like receptor (VRL-1) and P2X3 receptor was performed in the rat temporomandibular joint (TMJ). Blood vessels in the articular disk and capsule, the synovial membrane and the fibrous tissue around the condylar process were innervated by VR1- or P2X3 receptor-immunoreactive (ir) nerve fibers. However, VRL-1-immunoreactivity (ir) could not be detected in the TMJ. Retrograde tracing and immunohistochemical methods revealed that 25%, 41% and 52% of TMJ neurons in the trigeminal ganglion (TG) exhibited VR1-, VRL-1- and P2X3 receptor-ir, respectively. VR1-ir TMJ neurons were mostly small to medium-sized, whereas VRL-1- and P2X3 receptor-ir TMJ neurons were predominantly medium-sized to large. In addition, 73%, 28% and 44% of VR1-, VRL-1- and P2X3 receptor-ir TMJ neurons, respectively, coexpressed calcitonin gene-related peptide (CGRP)-ir. The present study suggests that the TMJ has abundant nociceptors which respond to vanilloid compounds, protons, heat and extracellular ATP.
KW - Immunohistochemistry
KW - P2X3
KW - Sensory systems
KW - Somatic and visceral afferents
KW - Temporomandibular joint
KW - Trigeminal ganglion
KW - VR1
KW - VRL-1
UR - http://www.scopus.com/inward/record.url?scp=1842815234&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1842815234&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2004.02.029
DO - 10.1016/j.brainres.2004.02.029
M3 - Article
C2 - 15081392
AN - SCOPUS:1842815234
VL - 1008
SP - 131
EP - 136
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1
ER -