VopV, an F-actin-binding type III secretion effector, is required for vibrio parahaemolyticus-induced enterotoxicity

Hirotaka Hiyoshi, Toshio Kodama, Kazunobu Saito, Kazuyoshi Goto, Shigeaki Matsuda, Yukihiro Akeda, Takeshi Honda, Tetsuya Iida

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Vibrio parahaemolyticus, a Gram-negative halophilic bacterium that causes acute gastroenteritis in humans, is characterized by two type III secretion systems (T3SS), namely T3SS1 and T3SS2. T3SS2 is indispensable for enterotoxicity but the effector(s) involved are unknown. Here, we identify VopV as a critical effector that is required to mediate V. parahaemolyticus T3SS2-dependent enterotoxicity. VopV was found to possess multiple F-actin-binding domains and the enterotoxicity caused by VopV correlated with its F-actin-binding activity. Furthermore, a T3SS2-related secretion system and a vopV homologous gene were also involved in the enterotoxicity of a non-O1/non-O139 V. cholerae strain. These results indicate that the F-actin-targeting effector VopV is involved in enterotoxic activity of T3SS2-possessing bacterial pathogens.

Original languageEnglish
Pages (from-to)401-409
Number of pages9
JournalCell Host and Microbe
Volume10
Issue number4
DOIs
Publication statusPublished - Oct 4 2011
Externally publishedYes

Fingerprint

Vibrio parahaemolyticus
Actins
Cholera
Gastroenteritis
Gram-Negative Bacteria
Genes

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Cancer Research
  • Molecular Biology

Cite this

VopV, an F-actin-binding type III secretion effector, is required for vibrio parahaemolyticus-induced enterotoxicity. / Hiyoshi, Hirotaka; Kodama, Toshio; Saito, Kazunobu; Goto, Kazuyoshi; Matsuda, Shigeaki; Akeda, Yukihiro; Honda, Takeshi; Iida, Tetsuya.

In: Cell Host and Microbe, Vol. 10, No. 4, 04.10.2011, p. 401-409.

Research output: Contribution to journalArticle

Hiyoshi, Hirotaka ; Kodama, Toshio ; Saito, Kazunobu ; Goto, Kazuyoshi ; Matsuda, Shigeaki ; Akeda, Yukihiro ; Honda, Takeshi ; Iida, Tetsuya. / VopV, an F-actin-binding type III secretion effector, is required for vibrio parahaemolyticus-induced enterotoxicity. In: Cell Host and Microbe. 2011 ; Vol. 10, No. 4. pp. 401-409.
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AU - Goto, Kazuyoshi

AU - Matsuda, Shigeaki

AU - Akeda, Yukihiro

AU - Honda, Takeshi

AU - Iida, Tetsuya

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