Volatilization of metal mercury from organomercurials by highly mercury-resistant Acidithiobacillus ferrooxidans MON-1

Tsuyoshi Sugio, Tomoe Komoda, Yuko Okazaki, Yuki Takeda, Sousaku Nakamura, Fumiaki Takeuchi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The iron-oxidizing bacterium Acidithiobacillus ferrooxidans MON-1 is highly resistant not only to mercuric chloride (HgCl2) but also to organomercurials such as methylmercury chloride (MMC). We have found that cytochrome c oxidase, purified from strain MON-1, reduces Hg2+ to volatilizable metal mercury (Hg0) with reduced mammalian cytochrome c or Fe2+ as an electron donor. In this study we found that cytochrome c oxidase can volatilize Hg0 from MMC as well as from Hg 2+ with reduced mammalian cytochrome c or c-type cytochrome purified from strain MON-1 as an electron donor. We also found that MMC-Hg0 volatilization activity is present in the MON-1 plasma membrane but not in the cytosol. These activities were strongly inhibited by sodium cyanide (NaCN) and the antibody produced against purified MON-1 cytochrome c oxidase. This is the first report to indicate that cytochrome c oxidase is involved in the degradation of organomercurials in microorganisms.

Original languageEnglish
Pages (from-to)1007-1012
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number5
DOIs
Publication statusPublished - 2010

Keywords

  • Acidithiobacillus ferrooxidans
  • Cytochrome c oxidase
  • Mercury reduction
  • Mercury resistance
  • Organomercurial

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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