Volatilization of Mercury under Acidic Conditions from Mercury-polluted Soil by a Mercury-resistant Acidithiobacillus ferrooxidans SUG 2-2

Fumiaki Takeuchi, Kenji Iwahori, Kazuo Kamimura, Atsunori Negishi, Terunobu Maeda, Tsuyoshi Sugio

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Volatilization of mercury under acidic conditions from soil polluted with mercuric chloride (1.5 mg Hg/kg soil) was studied with resting cells of a mercury-resistant strain, Acidithiobacillus ferrooxidans SUG 2-2. When resting cells of SUG 2-2 (0.01 mg of protein) were incubated for 10 d at 30°C in 20 ml of 1.6 mM sulfuric acid (pH 2.5) with ferrous sulfate (3%) and mercury-polluted soil (1 g), which contained 7.5 nmol of Hg, approximately 4.1 nmol of mercury was volatilized, indicating that 54% of the total mercury in the soil was volatilized. The amount of mercury volatilized from the soil was dependent on the concentration of Fe2+ added to the medium. When elemental sulfur, sodium tetrathionate, and pyrite were used as an electron donor for the mercury reduction, 16, 2.4 and 0.84%, respectively, of the total mercury added to the solution were volatilized. The optimum pH and temperature for mercury volatilization were 2.5 and 30°C. Approximately 92% of the total mercury in a salt solution (pH 2.5) with resting cells of SUG 2-2 (0.01 mg of protein), ferrous sulfate (3%) and mercury-polluted soil (1 g) was volatilized by further addition of both resting cells and Fe2+ and by incubating for 30 d at 30°C.

Original languageEnglish
Pages (from-to)1981-1986
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number9
DOIs
Publication statusPublished - Sep 2001

Fingerprint

Acidithiobacillus
Acidithiobacillus ferrooxidans
Volatilization
volatilization
Mercury
Vaporization
polluted soils
mercury
Soil
Soils
ferrous sulfate
Proteins
Pyrites
Tetrathionic Acid
Sulfuric acid
Sulfur
cells
iron
Sodium
mercuric chloride

Keywords

  • Acidithiobacillus ferrooxidans
  • Iron-oxidizing bacterium
  • Mercury
  • Resistance
  • Volatilization of mercury

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Volatilization of Mercury under Acidic Conditions from Mercury-polluted Soil by a Mercury-resistant Acidithiobacillus ferrooxidans SUG 2-2. / Takeuchi, Fumiaki; Iwahori, Kenji; Kamimura, Kazuo; Negishi, Atsunori; Maeda, Terunobu; Sugio, Tsuyoshi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 65, No. 9, 09.2001, p. 1981-1986.

Research output: Contribution to journalArticle

Takeuchi, Fumiaki ; Iwahori, Kenji ; Kamimura, Kazuo ; Negishi, Atsunori ; Maeda, Terunobu ; Sugio, Tsuyoshi. / Volatilization of Mercury under Acidic Conditions from Mercury-polluted Soil by a Mercury-resistant Acidithiobacillus ferrooxidans SUG 2-2. In: Bioscience, Biotechnology and Biochemistry. 2001 ; Vol. 65, No. 9. pp. 1981-1986.
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AU - Maeda, Terunobu

AU - Sugio, Tsuyoshi

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AB - Volatilization of mercury under acidic conditions from soil polluted with mercuric chloride (1.5 mg Hg/kg soil) was studied with resting cells of a mercury-resistant strain, Acidithiobacillus ferrooxidans SUG 2-2. When resting cells of SUG 2-2 (0.01 mg of protein) were incubated for 10 d at 30°C in 20 ml of 1.6 mM sulfuric acid (pH 2.5) with ferrous sulfate (3%) and mercury-polluted soil (1 g), which contained 7.5 nmol of Hg, approximately 4.1 nmol of mercury was volatilized, indicating that 54% of the total mercury in the soil was volatilized. The amount of mercury volatilized from the soil was dependent on the concentration of Fe2+ added to the medium. When elemental sulfur, sodium tetrathionate, and pyrite were used as an electron donor for the mercury reduction, 16, 2.4 and 0.84%, respectively, of the total mercury added to the solution were volatilized. The optimum pH and temperature for mercury volatilization were 2.5 and 30°C. Approximately 92% of the total mercury in a salt solution (pH 2.5) with resting cells of SUG 2-2 (0.01 mg of protein), ferrous sulfate (3%) and mercury-polluted soil (1 g) was volatilized by further addition of both resting cells and Fe2+ and by incubating for 30 d at 30°C.

KW - Acidithiobacillus ferrooxidans

KW - Iron-oxidizing bacterium

KW - Mercury

KW - Resistance

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