Vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress

Daisuke Ekuni, Takaaki Tomofuji, Toshihiro Sanbe, Koichiro Irie, Tetsuji Azuma, Takayuki Maruyama, Naofumi Tamaki, Jun Murakami, Susumu Kokeguchi, Tatsuo Yamamoto

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Objective: Periodontitis has been causally linked to cardiovascular disease, which is mediated through the oxidative stress induced by periodontitis. Since vitamin C has been suggested to limit oxidative damage, we hypothesized that vitamin C intake may reduce endothelial oxidative stress induced by periodontitis in the aorta. The aim of this study was to investigate the effects of vitamin C intake on the initiation of atherosclerosis in a ligature-induced rat periodontitis model. Design: Eighteen 8-week-old-male Wistar rats were divided into three groups of six rats and all rats received daily fresh water and powdered food through out the 6-week study. In the vitamin C and periodontitis groups, periodontitis was ligature-induced for the first 4 weeks. In the vitamin C group, rats were given distilled water containing 1 g/L vitamin C for the 2 weeks after removing the ligature. Results: In the periodontitis group, there was lipid deposition in the descending aorta and significant increases of serum level of hexanoyl-lysine (HEL), and aortic levels of nitrotyrosine expression, HEL expression and 8-hydroxydeoxyguanosine (8-OHdG) compared to the control group. Vitamin C intake significantly increased plasma vitamin C level and GSH:GSSG ratio (178% and 123%, respectively), and decreased level of serum HEL and aortic levels of nitrotyrosine, HEL and 8-OHdG (23%, 87%, 84%, and 38%, respectively). Conclusions: These results suggest that vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress.

Original languageEnglish
Pages (from-to)495-502
Number of pages8
JournalArchives of Oral Biology
Volume54
Issue number5
DOIs
Publication statusPublished - May 2009

Fingerprint

Periodontitis
Ascorbic Acid
Atherosclerosis
Oxidative Stress
Lysine
Ligation
Glutathione Disulfide
Fresh Water
Serum
Thoracic Aorta
Aorta
Wistar Rats
Cardiovascular Diseases
Lipids
Food
Control Groups
Water

Keywords

  • Atherosclerosis
  • Oxidative stress
  • Periodontal disease
  • Vitamin C

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

Cite this

Vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress. / Ekuni, Daisuke; Tomofuji, Takaaki; Sanbe, Toshihiro; Irie, Koichiro; Azuma, Tetsuji; Maruyama, Takayuki; Tamaki, Naofumi; Murakami, Jun; Kokeguchi, Susumu; Yamamoto, Tatsuo.

In: Archives of Oral Biology, Vol. 54, No. 5, 05.2009, p. 495-502.

Research output: Contribution to journalArticle

Ekuni, Daisuke ; Tomofuji, Takaaki ; Sanbe, Toshihiro ; Irie, Koichiro ; Azuma, Tetsuji ; Maruyama, Takayuki ; Tamaki, Naofumi ; Murakami, Jun ; Kokeguchi, Susumu ; Yamamoto, Tatsuo. / Vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress. In: Archives of Oral Biology. 2009 ; Vol. 54, No. 5. pp. 495-502.
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AB - Objective: Periodontitis has been causally linked to cardiovascular disease, which is mediated through the oxidative stress induced by periodontitis. Since vitamin C has been suggested to limit oxidative damage, we hypothesized that vitamin C intake may reduce endothelial oxidative stress induced by periodontitis in the aorta. The aim of this study was to investigate the effects of vitamin C intake on the initiation of atherosclerosis in a ligature-induced rat periodontitis model. Design: Eighteen 8-week-old-male Wistar rats were divided into three groups of six rats and all rats received daily fresh water and powdered food through out the 6-week study. In the vitamin C and periodontitis groups, periodontitis was ligature-induced for the first 4 weeks. In the vitamin C group, rats were given distilled water containing 1 g/L vitamin C for the 2 weeks after removing the ligature. Results: In the periodontitis group, there was lipid deposition in the descending aorta and significant increases of serum level of hexanoyl-lysine (HEL), and aortic levels of nitrotyrosine expression, HEL expression and 8-hydroxydeoxyguanosine (8-OHdG) compared to the control group. Vitamin C intake significantly increased plasma vitamin C level and GSH:GSSG ratio (178% and 123%, respectively), and decreased level of serum HEL and aortic levels of nitrotyrosine, HEL and 8-OHdG (23%, 87%, 84%, and 38%, respectively). Conclusions: These results suggest that vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress.

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