Vitamin B-12-deficiency affects immunoglobulin production and cytokine levels in mice

U. Funada, M. Wada, T. Kawata, K. Mori, H. Tamai, T. Isshiki, J. Onoda, N. Tanaka, T. Tadokoro, A. Maekawa

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Abstract

To clarify the role of B-12 in the immunological function, serum C3, IgM, IgG, IgE Contents, splenocytes expression of CD4, CD8, and CD4 positive intracellular IFN-γ and IL-4 were examined in B-12-deficient mice, and the effect of the administration of CH3-B-12 was also studied. Serum C3, IgM and IgG contents were lower in B-12-deficient mice than in the control mice. On the other hand, serum IgE content was sinificantly higher in B-12-deficient mice, and the value in CH3-B-12 administered mice, administered CH3-B-12 to B-12-deficient mice for 48 h before the end of feeding period, showed a tendency to recovery. CD4+CDS- cells and CD4+CDS-/CD4-CD8+ ratio in splenocytes were significantly higher in B-12-deficient mice than in control mice. CD4+IFN-γ+ cells was significantly lower in B-12-deficient mice than in control mice, and CD4+IL-4+ was significantly higher in B-12-deficient mice than in control mice. These results suggest that B-12-deficiency causes CD4+CDS-T cells shift from the T helper type 1 to the T helper type 2, which participate in the IgE production and elevates CD4+CDS-/CD4-CD8+ratio. Thus, B-12 plays a role in maintaining the immune function in mice.

Original languageEnglish
Pages (from-to)60-65
Number of pages6
JournalInternational Journal for Vitamin and Nutrition Research
Volume71
Issue number1
DOIs
Publication statusPublished - Jan 1 2001

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Keywords

  • B-12-deficient mice
  • CD4
  • CD8
  • IgE
  • IgG
  • Interferon-γ
  • Interleukin-4

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Funada, U., Wada, M., Kawata, T., Mori, K., Tamai, H., Isshiki, T., Onoda, J., Tanaka, N., Tadokoro, T., & Maekawa, A. (2001). Vitamin B-12-deficiency affects immunoglobulin production and cytokine levels in mice. International Journal for Vitamin and Nutrition Research, 71(1), 60-65. https://doi.org/10.1024/0300-9831.71.1.60