Vitamin B-12-deficiency affects immunoglobulin production and cytokine levels in mice

U. Funada, M. Wada, Tetsunori Kawata, K. Mori, H. Tamai, T. Isshiki, J. Onoda, N. Tanaka, T. Tadokoro, A. Maekawa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To clarify the role of B-12 in the immunological function, serum C3, IgM, IgG, IgE Contents, splenocytes expression of CD4, CD8, and CD4 positive intracellular IFN-γ and IL-4 were examined in B-12-deficient mice, and the effect of the administration of CH3-B-12 was also studied. Serum C3, IgM and IgG contents were lower in B-12-deficient mice than in the control mice. On the other hand, serum IgE content was sinificantly higher in B-12-deficient mice, and the value in CH3-B-12 administered mice, administered CH3-B-12 to B-12-deficient mice for 48 h before the end of feeding period, showed a tendency to recovery. CD4+CDS- cells and CD4+CDS-/CD4-CD8+ ratio in splenocytes were significantly higher in B-12-deficient mice than in control mice. CD4+IFN-γ+ cells was significantly lower in B-12-deficient mice than in control mice, and CD4+IL-4+ was significantly higher in B-12-deficient mice than in control mice. These results suggest that B-12-deficiency causes CD4+CDS-T cells shift from the T helper type 1 to the T helper type 2, which participate in the IgE production and elevates CD4+CDS-/CD4-CD8+ratio. Thus, B-12 plays a role in maintaining the immune function in mice.

Original languageEnglish
Pages (from-to)60-65
Number of pages6
JournalInternational Journal for Vitamin and Nutrition Research
Volume71
Issue number1
Publication statusPublished - 2001

Fingerprint

Vitamin B 12 Deficiency
vitamin B12
immunoglobulins
Immunoglobulins
cytokines
Cytokines
mice
Immunoglobulin E
CD4-CD8 Ratio
interleukin-4
splenocytes
Interleukin-4
Immunoglobulin M
Immunoglobulin G
Serum

Keywords

  • B-12-deficient mice
  • CD4
  • CD8
  • IgE
  • IgG
  • Interferon-γ
  • Interleukin-4

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Vitamin B-12-deficiency affects immunoglobulin production and cytokine levels in mice. / Funada, U.; Wada, M.; Kawata, Tetsunori; Mori, K.; Tamai, H.; Isshiki, T.; Onoda, J.; Tanaka, N.; Tadokoro, T.; Maekawa, A.

In: International Journal for Vitamin and Nutrition Research, Vol. 71, No. 1, 2001, p. 60-65.

Research output: Contribution to journalArticle

Funada, U, Wada, M, Kawata, T, Mori, K, Tamai, H, Isshiki, T, Onoda, J, Tanaka, N, Tadokoro, T & Maekawa, A 2001, 'Vitamin B-12-deficiency affects immunoglobulin production and cytokine levels in mice', International Journal for Vitamin and Nutrition Research, vol. 71, no. 1, pp. 60-65.
Funada, U. ; Wada, M. ; Kawata, Tetsunori ; Mori, K. ; Tamai, H. ; Isshiki, T. ; Onoda, J. ; Tanaka, N. ; Tadokoro, T. ; Maekawa, A. / Vitamin B-12-deficiency affects immunoglobulin production and cytokine levels in mice. In: International Journal for Vitamin and Nutrition Research. 2001 ; Vol. 71, No. 1. pp. 60-65.
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AU - Onoda, J.

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