Selective cyclooxygenase-2 inhibitor prevents cisplatin-induced tumorigenesis in A/J mice

Toshiaki Okada, Nagio Takigawa, Daizo Kishino, Hideki Katayama, Shouichi Kuyama, Ken Sato, Junko Mimoto, Hiroshi Ueoka, Mitsune Tanimoto, Katsuyuki Kiura

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Abstract

Cisplatin is used to treat lung cancer; however, it is also a known carcinogen. Cyclooxygenase-2 (COX-2) inhibitors have been shown to prevent carcinogen-induced experimental tumors. We investi-gated the effect of a COX-2 inhibitor, celecoxib, on cisplatin-induced lung tumors. One hundred twenty 4-week-old A/J mice were divided into 6 groups: group 1, no treatment; group 2, low-dose celecoxib (150mg/kg); group 3, high-dose celecoxib (l,500mg/kg); group 4, cisplatin alone; group 5, cisplatin plus low-dose celecoxib; and group 6, cisplatin plus high-dose celecoxib. Mice in groups 4-6 were administered cisplatin (1.62mg/kg, i.p.) once a week for 10 weeks between 7 and 16 weeks of age. All mice were sacrificed at week 30. Tumor incidence was 15.8% in group 1, 25% in group 2, 26.3% in group 3, 60% in group 4, 50% in group 5, and 50% in group 6. Tumor multiplicity was 0.2, 0.3, 0.3, 1.3, 1.0, and 0.6 in groups 1-6, respectively. Tumor multiplicity in the cisplatin-treated mice was reduced by celecoxib treatment in a dose-dependent manner (p < 0.05, group 4 vs. group 6). Celecoxib significantly reduced COX-2 expression in cisplatin-induced tumors (p < 0.01, group 4 vs. group 6).

Original languageEnglish
Pages (from-to)203-211
Number of pages9
JournalActa medica Okayama
Volume66
Issue number3
Publication statusPublished - 2012

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Keywords

  • Celecoxib
  • Chemoprevention
  • Cisplatin
  • Cyclooxygenase-2
  • Non-small cell lung cancer

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Okada, T., Takigawa, N., Kishino, D., Katayama, H., Kuyama, S., Sato, K., Mimoto, J., Ueoka, H., Tanimoto, M., & Kiura, K. (2012). Selective cyclooxygenase-2 inhibitor prevents cisplatin-induced tumorigenesis in A/J mice. Acta medica Okayama, 66(3), 203-211.