Visualization of plastids in pollen grains: Involvement of FtsZ1 in pollen plastid division

Lay Yin Tang, Noriko Nagata, Ryo Matsushima, Yuling Chen, Yasushi Yoshioka, Wataru Sakamoto

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Visualizing organelles in living cells is a powerful method to analyze their intrinsic mechanisms. Easy observation of chlorophyll facilitates the study of the underlying mechanisms in chloroplasts, but not in other plastid types. Here, we constructed a transgenic plant enabling visualization of plastids in pollen grains. Combination of a plastid-targeted fluorescent protein with a pollen-specific promoter allowed us to observe the precise number, size and morphology of plastids in pollen grains of the wild type and the ftsZ1 mutant, whose responsible gene plays a central role in chloroplast division. The transgenic material presented in this work is useful for studying the division mechanism of pollen plastids.

Original languageEnglish
Pages (from-to)904-908
Number of pages5
JournalPlant and Cell Physiology
Volume50
Issue number4
DOIs
Publication statusPublished - Apr 2009

Keywords

  • Fluorescent protein
  • FtsZ1
  • Male gametophyte
  • Plastid division
  • Pollen

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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