Visualization of plastids in pollen grains

Involvement of FtsZ1 in pollen plastid division

Lay Yin Tang, Noriko Nagata, Ryo Matsushima, Yuling Chen, Yasushi Yoshioka, Wataru Sakamoto

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Visualizing organelles in living cells is a powerful method to analyze their intrinsic mechanisms. Easy observation of chlorophyll facilitates the study of the underlying mechanisms in chloroplasts, but not in other plastid types. Here, we constructed a transgenic plant enabling visualization of plastids in pollen grains. Combination of a plastid-targeted fluorescent protein with a pollen-specific promoter allowed us to observe the precise number, size and morphology of plastids in pollen grains of the wild type and the ftsZ1 mutant, whose responsible gene plays a central role in chloroplast division. The transgenic material presented in this work is useful for studying the division mechanism of pollen plastids.

Original languageEnglish
Pages (from-to)904-908
Number of pages5
JournalPlant and Cell Physiology
Volume50
Issue number4
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Plastids
Pollen
plastids
pollen
Chloroplasts
chloroplasts
Genetically Modified Plants
Chlorophyll
Organelles
organelles
transgenic plants
promoter regions
Observation
genetically modified organisms
chlorophyll
mutants
Genes
Proteins
genes
cells

Keywords

  • Fluorescent protein
  • FtsZ1
  • Male gametophyte
  • Plastid division
  • Pollen

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Visualization of plastids in pollen grains : Involvement of FtsZ1 in pollen plastid division. / Tang, Lay Yin; Nagata, Noriko; Matsushima, Ryo; Chen, Yuling; Yoshioka, Yasushi; Sakamoto, Wataru.

In: Plant and Cell Physiology, Vol. 50, No. 4, 04.2009, p. 904-908.

Research output: Contribution to journalArticle

Tang, Lay Yin ; Nagata, Noriko ; Matsushima, Ryo ; Chen, Yuling ; Yoshioka, Yasushi ; Sakamoto, Wataru. / Visualization of plastids in pollen grains : Involvement of FtsZ1 in pollen plastid division. In: Plant and Cell Physiology. 2009 ; Vol. 50, No. 4. pp. 904-908.
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