Visualization of diffuse centromeres with centromere-specific histone H3 in the holocentric plant Luzula nivea

Kiyotaka Nagaki, Kazunari Kashihara, Minoru Murata

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Although holocentric species are scattered throughout the plant and animal kingdoms, only holocentric chromosomes of the nematode worm Caenorhabditis elegans have been analyzed with centromeric protein markers. In an effort to determine the holocentric structure in plants, we investigated the snowy woodrush Luzula nivea. From the young roots, a cDNA encoding a putative centromere-specific histone H3 (LnCENH3) was successfully isolated based on sequence similarity among plant CENH3s. The deduced amino acid sequence was then used to raise an anti-LnCENH3 antibody. Immunostaining clearly revealed the diffuse centromere-like structure that appears in the linear shape at prophase to telophase. Furthermore, it was shown that the amount of LnCENH3 decreased significantly at interphase. The polar side positioning on each chromatid at metaphase to anaphase also confirmed that LnCENH3 represents one of the centromere-specific proteins in L. nivea. These data from L. nivea are compared with those from C. elegans, and common features of holocentric chromosomes are discussed.

Original languageEnglish
Pages (from-to)1886-1893
Number of pages8
JournalPlant Cell
Volume17
Issue number7
DOIs
Publication statusPublished - 2005

Fingerprint

Luzula
Centromere
centromeres
Chromosomes
histones
Histones
Visualization
Caenorhabditis elegans
Telophase
chromosomes
Plant Structures
Prophase
Anti-Idiotypic Antibodies
telophase
Anaphase
Animals
Proteins
Chromatids
chromatids
Complementary DNA

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Visualization of diffuse centromeres with centromere-specific histone H3 in the holocentric plant Luzula nivea. / Nagaki, Kiyotaka; Kashihara, Kazunari; Murata, Minoru.

In: Plant Cell, Vol. 17, No. 7, 2005, p. 1886-1893.

Research output: Contribution to journalArticle

@article{1d74e69de3964226af5fe1e068850f0a,
title = "Visualization of diffuse centromeres with centromere-specific histone H3 in the holocentric plant Luzula nivea",
abstract = "Although holocentric species are scattered throughout the plant and animal kingdoms, only holocentric chromosomes of the nematode worm Caenorhabditis elegans have been analyzed with centromeric protein markers. In an effort to determine the holocentric structure in plants, we investigated the snowy woodrush Luzula nivea. From the young roots, a cDNA encoding a putative centromere-specific histone H3 (LnCENH3) was successfully isolated based on sequence similarity among plant CENH3s. The deduced amino acid sequence was then used to raise an anti-LnCENH3 antibody. Immunostaining clearly revealed the diffuse centromere-like structure that appears in the linear shape at prophase to telophase. Furthermore, it was shown that the amount of LnCENH3 decreased significantly at interphase. The polar side positioning on each chromatid at metaphase to anaphase also confirmed that LnCENH3 represents one of the centromere-specific proteins in L. nivea. These data from L. nivea are compared with those from C. elegans, and common features of holocentric chromosomes are discussed.",
author = "Kiyotaka Nagaki and Kazunari Kashihara and Minoru Murata",
year = "2005",
doi = "10.1105/tpc.105.032961",
language = "English",
volume = "17",
pages = "1886--1893",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "7",

}

TY - JOUR

T1 - Visualization of diffuse centromeres with centromere-specific histone H3 in the holocentric plant Luzula nivea

AU - Nagaki, Kiyotaka

AU - Kashihara, Kazunari

AU - Murata, Minoru

PY - 2005

Y1 - 2005

N2 - Although holocentric species are scattered throughout the plant and animal kingdoms, only holocentric chromosomes of the nematode worm Caenorhabditis elegans have been analyzed with centromeric protein markers. In an effort to determine the holocentric structure in plants, we investigated the snowy woodrush Luzula nivea. From the young roots, a cDNA encoding a putative centromere-specific histone H3 (LnCENH3) was successfully isolated based on sequence similarity among plant CENH3s. The deduced amino acid sequence was then used to raise an anti-LnCENH3 antibody. Immunostaining clearly revealed the diffuse centromere-like structure that appears in the linear shape at prophase to telophase. Furthermore, it was shown that the amount of LnCENH3 decreased significantly at interphase. The polar side positioning on each chromatid at metaphase to anaphase also confirmed that LnCENH3 represents one of the centromere-specific proteins in L. nivea. These data from L. nivea are compared with those from C. elegans, and common features of holocentric chromosomes are discussed.

AB - Although holocentric species are scattered throughout the plant and animal kingdoms, only holocentric chromosomes of the nematode worm Caenorhabditis elegans have been analyzed with centromeric protein markers. In an effort to determine the holocentric structure in plants, we investigated the snowy woodrush Luzula nivea. From the young roots, a cDNA encoding a putative centromere-specific histone H3 (LnCENH3) was successfully isolated based on sequence similarity among plant CENH3s. The deduced amino acid sequence was then used to raise an anti-LnCENH3 antibody. Immunostaining clearly revealed the diffuse centromere-like structure that appears in the linear shape at prophase to telophase. Furthermore, it was shown that the amount of LnCENH3 decreased significantly at interphase. The polar side positioning on each chromatid at metaphase to anaphase also confirmed that LnCENH3 represents one of the centromere-specific proteins in L. nivea. These data from L. nivea are compared with those from C. elegans, and common features of holocentric chromosomes are discussed.

UR - http://www.scopus.com/inward/record.url?scp=28644441122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28644441122&partnerID=8YFLogxK

U2 - 10.1105/tpc.105.032961

DO - 10.1105/tpc.105.032961

M3 - Article

C2 - 15937225

AN - SCOPUS:28644441122

VL - 17

SP - 1886

EP - 1893

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 7

ER -