Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane

Kazuhito V. Tabata, Ken Sato, Toru Ide, Takayuki Nishizaka, Akihiko Nakano, Hiroyuki Noji

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Selective protein export from the endoplasmic reticulum is mediated by COPII vesicles. Here, we investigated the dynamics of fluorescently labelled cargo and non-cargo proteins during COPII vesicle formation using single-molecule microscopy combined with an artificial planar lipid bilayer. Single-molecule analysis showed that the Sar1p-Sec23/24p-cargo complex, but not the Sar1p-Sec23/24p complex, undergoes partial dimerization before Sec13/31p recruitment. On addition of a complete COPII mixture, cargo molecules start to assemble into fluorescent spots and clusters followed by vesicle release from the planar membrane. We show that continuous GTPase cycles of Sar1p facilitate cargo concentration into COPII vesicle buds, and at the same time, non-cargo proteins are excluded from cargo clusters. We propose that the minimal set of COPII components is required not only to concentrate cargo molecules, but also to mediate exclusion of non-cargo proteins from the COPII vesicles.

Original languageEnglish
Pages (from-to)3279-3289
Number of pages11
JournalEMBO Journal
Volume28
Issue number21
DOIs
Publication statusPublished - Nov 2009
Externally publishedYes

Fingerprint

Membrane Lipids
Machinery
Visualization
Molecules
Proteins
Lipid bilayers
Dimerization
GTP Phosphohydrolases
Lipid Bilayers
Endoplasmic Reticulum
Microscopic examination
Membranes

Keywords

  • Cargo packaging
  • COPII
  • Transport vesicle
  • Vesicle transport

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane. / Tabata, Kazuhito V.; Sato, Ken; Ide, Toru; Nishizaka, Takayuki; Nakano, Akihiko; Noji, Hiroyuki.

In: EMBO Journal, Vol. 28, No. 21, 11.2009, p. 3279-3289.

Research output: Contribution to journalArticle

Tabata, KV, Sato, K, Ide, T, Nishizaka, T, Nakano, A & Noji, H 2009, 'Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane', EMBO Journal, vol. 28, no. 21, pp. 3279-3289. https://doi.org/10.1038/emboj.2009.269
Tabata, Kazuhito V. ; Sato, Ken ; Ide, Toru ; Nishizaka, Takayuki ; Nakano, Akihiko ; Noji, Hiroyuki. / Visualization of cargo concentration by COPII minimal machinery in a planar lipid membrane. In: EMBO Journal. 2009 ; Vol. 28, No. 21. pp. 3279-3289.
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