Abstract
Supernumerary chromosomes, termed 'conditionally dispensable' (CD) chromosomes, are known in Nectria haematococca. Because these CD chromosomes had been revealed solely by pulsed-field gel electrophoresis, their morphological properties were unknown. In this study, we visualized a 1.6-Mb CD chromosome of this fungus by three different types of fluorescence in situ hybridization. The CD chromosome at mitotic metaphase was similar in its appearance to the other chromosomes in the genome. Heterochromatic condensation was not distinct in the CD chromosome, suggesting that it is primarily euchromatic. It was also evident that the CD chromosome is unique and not a duplicate of other chromosomes in the genome. At interphase and prophase, the CD chromosome was not dispersed throughout the nucleus, but occupied a limited domain. Occasionally, occurrence of two distinct unattached copies of the CD chromosome were observed during interphase and metaphase.
| Original language | English |
|---|---|
| Pages (from-to) | 169-177 |
| Number of pages | 9 |
| Journal | Fungal Genetics and Biology |
| Volume | 26 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Apr 1999 |
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Keywords
- B chromosome
- Conditionally dispensable chromosome
- Supernumerary chromosome
ASJC Scopus subject areas
- Genetics
- Microbiology
Cite this
Visualization of a conditionally dispensable chromosome in the filamentous ascomycete Nectria haematococca by fluorescence in situ hybridization. / Taga, Masatoki; Murata, Minoru; Vanetten, Hans D.
In: Fungal Genetics and Biology, Vol. 26, No. 3, 04.1999, p. 169-177.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Visualization of a conditionally dispensable chromosome in the filamentous ascomycete Nectria haematococca by fluorescence in situ hybridization
AU - Taga, Masatoki
AU - Murata, Minoru
AU - Vanetten, Hans D.
PY - 1999/4
Y1 - 1999/4
N2 - Supernumerary chromosomes, termed 'conditionally dispensable' (CD) chromosomes, are known in Nectria haematococca. Because these CD chromosomes had been revealed solely by pulsed-field gel electrophoresis, their morphological properties were unknown. In this study, we visualized a 1.6-Mb CD chromosome of this fungus by three different types of fluorescence in situ hybridization. The CD chromosome at mitotic metaphase was similar in its appearance to the other chromosomes in the genome. Heterochromatic condensation was not distinct in the CD chromosome, suggesting that it is primarily euchromatic. It was also evident that the CD chromosome is unique and not a duplicate of other chromosomes in the genome. At interphase and prophase, the CD chromosome was not dispersed throughout the nucleus, but occupied a limited domain. Occasionally, occurrence of two distinct unattached copies of the CD chromosome were observed during interphase and metaphase.
AB - Supernumerary chromosomes, termed 'conditionally dispensable' (CD) chromosomes, are known in Nectria haematococca. Because these CD chromosomes had been revealed solely by pulsed-field gel electrophoresis, their morphological properties were unknown. In this study, we visualized a 1.6-Mb CD chromosome of this fungus by three different types of fluorescence in situ hybridization. The CD chromosome at mitotic metaphase was similar in its appearance to the other chromosomes in the genome. Heterochromatic condensation was not distinct in the CD chromosome, suggesting that it is primarily euchromatic. It was also evident that the CD chromosome is unique and not a duplicate of other chromosomes in the genome. At interphase and prophase, the CD chromosome was not dispersed throughout the nucleus, but occupied a limited domain. Occasionally, occurrence of two distinct unattached copies of the CD chromosome were observed during interphase and metaphase.
KW - B chromosome
KW - Conditionally dispensable chromosome
KW - Supernumerary chromosome
UR - http://www.scopus.com/inward/record.url?scp=0032854437&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032854437&partnerID=8YFLogxK
U2 - 10.1006/fgbi.1999.1123
DO - 10.1006/fgbi.1999.1123
M3 - Article
C2 - 10361031
AN - SCOPUS:0032854437
VL - 26
SP - 169
EP - 177
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
SN - 1087-1845
IS - 3
ER -