Visual evoked potential in rabbits’ eyes with subretinal implantation by vitrectomy of Okayama university-type retinal prosthesis (OURePTM)

Toshihiko Matsuo, Tetsuya Uchida, Koichiro Yamashita, Shigiko Takei, Daisuke Ido, Mamoru Tanaka, Masao Oguchi, Toshinori Furukawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Okayama University-type retinal prosthesis (OURePTM) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to test surgical feasibility for subretinal film implantation and to examine functional durability of films in subretinal space. Dye-coupled films were implanted subretinally by vitrectomy in the right eye of normal white rabbits: 8 rabbits for 1 month and 8 rabbits for 6 months. The implanted films were removed by vitrectomy in 4 of these 8 rabbits in 1-month or 6-month implantation group. The films were also implanted in 4 rhodopsin-transgenic retinal dystrophic rabbits. Visual evoked potential was measured before film implantation as well as 1 or 6 months after film implantation, or 1 month after film removal. The films were successfully implanted in subretinal space of retinal detachment induced by subretinal fluid injection with a 38G polyimide tip. The retina was reattached by fluid-air exchange in vitreous cavity, retinal laser coagulation, and silicone oil injection. The ratios of P2 amplitudes of visual evoked potential in the implanted right eye over control left eye did not show significant changes between pre-implantation and post-implantation or post-removal (paired t-test). In Kelvin probe measurements, 4 pieces each of removed films which were implanted for 1 or 6 months showed proportional increase of surface electric potential in response to increasing light intensity. The film implantation was safe and implanted films were capable of responding to light.

Original languageEnglish
Pages (from-to)247-259
Number of pages13
JournalJournal of Veterinary Medical Science
Volume80
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

Fingerprint

Visual Prosthesis
prostheses
evoked potentials
Visual Evoked Potentials
Vitrectomy
films (materials)
eyes
rabbits
Rabbits
Light
Coloring Agents
Laser Coagulation
Subretinal Fluid
Silicone Oils
Injections
Rhodopsin
Polyethylene
Retinal Detachment
Retina
Air

Keywords

  • Dye-coupled thin film retinal prosthesis
  • Rabbit
  • Retinal dystrophy (retinitis pigmentosa)
  • Visual evoked potential
  • Vitrectomy

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Visual evoked potential in rabbits’ eyes with subretinal implantation by vitrectomy of Okayama university-type retinal prosthesis (OURePTM). / Matsuo, Toshihiko; Uchida, Tetsuya; Yamashita, Koichiro; Takei, Shigiko; Ido, Daisuke; Tanaka, Mamoru; Oguchi, Masao; Furukawa, Toshinori.

In: Journal of Veterinary Medical Science, Vol. 80, No. 2, 01.02.2018, p. 247-259.

Research output: Contribution to journalArticle

Matsuo, Toshihiko ; Uchida, Tetsuya ; Yamashita, Koichiro ; Takei, Shigiko ; Ido, Daisuke ; Tanaka, Mamoru ; Oguchi, Masao ; Furukawa, Toshinori. / Visual evoked potential in rabbits’ eyes with subretinal implantation by vitrectomy of Okayama university-type retinal prosthesis (OURePTM). In: Journal of Veterinary Medical Science. 2018 ; Vol. 80, No. 2. pp. 247-259.
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