TY - JOUR
T1 - Visual evoked potential in rabbits’ eyes with subretinal implantation by vitrectomy of Okayama university-type retinal prosthesis (OURePTM)
AU - Matsuo, Toshihiko
AU - Uchida, Tetsuya
AU - Yamashita, Koichiro
AU - Takei, Shigiko
AU - Ido, Daisuke
AU - Tanaka, Mamoru
AU - Oguchi, Masao
AU - Furukawa, Toshinori
N1 - Funding Information:
ACKNOWLEDGMENTS. This study was supported by a grant for the Translational Research Network Program from the Japan Agency for Medical Research and Development (AMED). The authors declare that they have no conflict of interest. We thank Chie Matsuo, DDS, PhD, for statistical analysis and preparation of figures.
Publisher Copyright:
© 2018 The Japanese Society of Veterinary Science.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Okayama University-type retinal prosthesis (OURePTM) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to test surgical feasibility for subretinal film implantation and to examine functional durability of films in subretinal space. Dye-coupled films were implanted subretinally by vitrectomy in the right eye of normal white rabbits: 8 rabbits for 1 month and 8 rabbits for 6 months. The implanted films were removed by vitrectomy in 4 of these 8 rabbits in 1-month or 6-month implantation group. The films were also implanted in 4 rhodopsin-transgenic retinal dystrophic rabbits. Visual evoked potential was measured before film implantation as well as 1 or 6 months after film implantation, or 1 month after film removal. The films were successfully implanted in subretinal space of retinal detachment induced by subretinal fluid injection with a 38G polyimide tip. The retina was reattached by fluid-air exchange in vitreous cavity, retinal laser coagulation, and silicone oil injection. The ratios of P2 amplitudes of visual evoked potential in the implanted right eye over control left eye did not show significant changes between pre-implantation and post-implantation or post-removal (paired t-test). In Kelvin probe measurements, 4 pieces each of removed films which were implanted for 1 or 6 months showed proportional increase of surface electric potential in response to increasing light intensity. The film implantation was safe and implanted films were capable of responding to light.
AB - Okayama University-type retinal prosthesis (OURePTM) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to test surgical feasibility for subretinal film implantation and to examine functional durability of films in subretinal space. Dye-coupled films were implanted subretinally by vitrectomy in the right eye of normal white rabbits: 8 rabbits for 1 month and 8 rabbits for 6 months. The implanted films were removed by vitrectomy in 4 of these 8 rabbits in 1-month or 6-month implantation group. The films were also implanted in 4 rhodopsin-transgenic retinal dystrophic rabbits. Visual evoked potential was measured before film implantation as well as 1 or 6 months after film implantation, or 1 month after film removal. The films were successfully implanted in subretinal space of retinal detachment induced by subretinal fluid injection with a 38G polyimide tip. The retina was reattached by fluid-air exchange in vitreous cavity, retinal laser coagulation, and silicone oil injection. The ratios of P2 amplitudes of visual evoked potential in the implanted right eye over control left eye did not show significant changes between pre-implantation and post-implantation or post-removal (paired t-test). In Kelvin probe measurements, 4 pieces each of removed films which were implanted for 1 or 6 months showed proportional increase of surface electric potential in response to increasing light intensity. The film implantation was safe and implanted films were capable of responding to light.
KW - Dye-coupled thin film retinal prosthesis
KW - Rabbit
KW - Retinal dystrophy (retinitis pigmentosa)
KW - Visual evoked potential
KW - Vitrectomy
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U2 - 10.1292/jvms.17-0422
DO - 10.1292/jvms.17-0422
M3 - Article
C2 - 29311491
AN - SCOPUS:85041736853
SN - 0916-7250
VL - 80
SP - 247
EP - 259
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
IS - 2
ER -