Virus purification by CsCl density gradient using general centrifugation

Tadahiro Nasukawa, Jumpei Uchiyama, Satoshi Taharaguchi, Sumire Ota, Takako Ujihara, Shigenobu Matsuzaki, Hironobu Murakami, Keijirou Mizukami, Masahiro Sakaguchi

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Virus purification by cesium chloride (CsCl) density gradient, which generally requires an expensive ultracentrifuge, is an essential technique in virology. Here, we optimized virus purification by CsCl density gradient using general centrifugation (40,000 × g, 2 h, 4 °C), which showed almost the same purification ability as conventional CsCl density gradient ultracentrifugation (100,000 × g, 1 h, 4 °C) using phages S13′ and φEF24C. Moreover, adenovirus strain JM1/1 was also successfully purified by this method. We suggest that general centrifugation can become a less costly alternative to ultracentrifugation for virus purification by CsCl densiy gradient and will thus encourage research in virology.

Original languageEnglish
Pages (from-to)3523-3528
Number of pages6
JournalArchives of Virology
Volume162
Issue number11
DOIs
Publication statusPublished - Nov 1 2017
Externally publishedYes

Keywords

  • CsCl density gradient
  • General centrifugation
  • Virus purification

ASJC Scopus subject areas

  • Virology

Fingerprint

Dive into the research topics of 'Virus purification by CsCl density gradient using general centrifugation'. Together they form a unique fingerprint.

Cite this