Virus-mediated transduction of apolipoprotein E (ApoE)-Sendai develops lipoprotein glomerulopathy in ApoE-deficient mice

Yasushi Ishigaki, Shinichi Oikawa, Takashi Suzuki, Shinichi Usui, Kenta Magoori, Dong Ho Kim, Hiroyuki Suzuki, Jun Sasaki, Hironobu Sasano, Mitsuyo Okazaki, Takayoshi Toyota, Takao Saito, Tokuo T. Yamamoto

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Lipoprotein glomerulopathy (LPG) is a unique renal disease characterized by thrombus-like substances in markedly dilated glomerular capillaries, dysbetalipoproteinemia, and elevated plasma concentrations of apoE. Recent studies identified several apoE mutations in patients with LPG, including apoE2(R145P) Sendal (apoE-Sendai). Virus-mediated transduction of apoE-Sendai in apoE-deficient hypereholesterolemic mice resulted in insufficient correction of hypercholesterolemia and a marked and temporal induction of plasma triglyceride levels. In vitro binding studies showed that apoE-Sendal has a reduced affinity for the low density lipoprotein receptor, suggesting that dysbetalipoproteinemia in LPG is caused by the apoE mutation. Furthermore, histological examination revealed marked intraglomerular depositions of apoE-containing lipoproteins in mice injected with apoE-Sendai virus. These LPG-like depositions were detected 6 days after virus injection and were sustained for at least 69 days. Our results demonstrated that apoE-Sendai is an etiological cause of LPG.

Original languageEnglish
Pages (from-to)31269-31273
Number of pages5
JournalJournal of Biological Chemistry
Volume275
Issue number40
Publication statusPublished - Oct 6 2000
Externally publishedYes

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Apolipoproteins E
Viruses
Lipoproteins
Hyperlipoproteinemia Type III
Apolipoprotein E2
Lipoprotein Glomerulopathy
Plasmas
Sendai virus
Mutation
LDL Receptors
Hypercholesterolemia
Triglycerides
Thrombosis
Kidney
Injections

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ishigaki, Y., Oikawa, S., Suzuki, T., Usui, S., Magoori, K., Kim, D. H., ... Yamamoto, T. T. (2000). Virus-mediated transduction of apolipoprotein E (ApoE)-Sendai develops lipoprotein glomerulopathy in ApoE-deficient mice. Journal of Biological Chemistry, 275(40), 31269-31273.

Virus-mediated transduction of apolipoprotein E (ApoE)-Sendai develops lipoprotein glomerulopathy in ApoE-deficient mice. / Ishigaki, Yasushi; Oikawa, Shinichi; Suzuki, Takashi; Usui, Shinichi; Magoori, Kenta; Kim, Dong Ho; Suzuki, Hiroyuki; Sasaki, Jun; Sasano, Hironobu; Okazaki, Mitsuyo; Toyota, Takayoshi; Saito, Takao; Yamamoto, Tokuo T.

In: Journal of Biological Chemistry, Vol. 275, No. 40, 06.10.2000, p. 31269-31273.

Research output: Contribution to journalArticle

Ishigaki, Y, Oikawa, S, Suzuki, T, Usui, S, Magoori, K, Kim, DH, Suzuki, H, Sasaki, J, Sasano, H, Okazaki, M, Toyota, T, Saito, T & Yamamoto, TT 2000, 'Virus-mediated transduction of apolipoprotein E (ApoE)-Sendai develops lipoprotein glomerulopathy in ApoE-deficient mice', Journal of Biological Chemistry, vol. 275, no. 40, pp. 31269-31273.
Ishigaki, Yasushi ; Oikawa, Shinichi ; Suzuki, Takashi ; Usui, Shinichi ; Magoori, Kenta ; Kim, Dong Ho ; Suzuki, Hiroyuki ; Sasaki, Jun ; Sasano, Hironobu ; Okazaki, Mitsuyo ; Toyota, Takayoshi ; Saito, Takao ; Yamamoto, Tokuo T. / Virus-mediated transduction of apolipoprotein E (ApoE)-Sendai develops lipoprotein glomerulopathy in ApoE-deficient mice. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 40. pp. 31269-31273.
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