Abstract
Porphyromonas gingivalis is a periodontal pathogen whose fimbriae are classified into six genotypes based on the diversity of the fimA genes encoding each fimbria subunit. It was suggested that P. gingivalis strains with type II fimbriae were more virulent than type I strains. For the present study, we generated the mutants in which fimA was substituted with different genotypes to study virulence of type II fimbriae. Using plasmid vectors, fimA of ATCC33277 (type I strain) was substituted with type II fimA, and that of OMZ314 (type II strain) with type I fimA. The substitution of type I fimA with type II enhanced bacterial adhesion/invasion to epithelial cells, whereas substitution with type I fimA resulted in diminished efficiency. Following bacterial invasion, type II clones swiftly degraded cellular paxillin and focal adhesion kinase, and inhibited cellular migration, whereas type I clones and ΔfimA mutants did not. BIAcore analysis demonstrated that type II fimbriae possess greater adhesive abilities for their receptor α5β1-integrin than those of type I. In a mouse abscess model, the type II clones significantly induced serum IL-1β and IL-6, as well as other infectious symptoms. These results suggest that type II fimbriae are a critical determinant of P. gingivalis virulence.
| Original language | English |
|---|---|
| Pages (from-to) | 753-765 |
| Number of pages | 13 |
| Journal | Cellular Microbiology |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2007 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Microbiology
Cite this
Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype. / Kato, Takahiro; Kawai, Shinji; Nakano, Kazuhiko; Inaba, Hiroaki; Kuboniwa, Masae; Nakagawa, Ichiro; Tsuda, Kayoko; Omori, Hiroko; Ooshima, Takashi; Yoshimori, Tamotsu; Amano, Atsuo.
In: Cellular Microbiology, Vol. 9, No. 3, 03.2007, p. 753-765.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype
AU - Kato, Takahiro
AU - Kawai, Shinji
AU - Nakano, Kazuhiko
AU - Inaba, Hiroaki
AU - Kuboniwa, Masae
AU - Nakagawa, Ichiro
AU - Tsuda, Kayoko
AU - Omori, Hiroko
AU - Ooshima, Takashi
AU - Yoshimori, Tamotsu
AU - Amano, Atsuo
PY - 2007/3
Y1 - 2007/3
N2 - Porphyromonas gingivalis is a periodontal pathogen whose fimbriae are classified into six genotypes based on the diversity of the fimA genes encoding each fimbria subunit. It was suggested that P. gingivalis strains with type II fimbriae were more virulent than type I strains. For the present study, we generated the mutants in which fimA was substituted with different genotypes to study virulence of type II fimbriae. Using plasmid vectors, fimA of ATCC33277 (type I strain) was substituted with type II fimA, and that of OMZ314 (type II strain) with type I fimA. The substitution of type I fimA with type II enhanced bacterial adhesion/invasion to epithelial cells, whereas substitution with type I fimA resulted in diminished efficiency. Following bacterial invasion, type II clones swiftly degraded cellular paxillin and focal adhesion kinase, and inhibited cellular migration, whereas type I clones and ΔfimA mutants did not. BIAcore analysis demonstrated that type II fimbriae possess greater adhesive abilities for their receptor α5β1-integrin than those of type I. In a mouse abscess model, the type II clones significantly induced serum IL-1β and IL-6, as well as other infectious symptoms. These results suggest that type II fimbriae are a critical determinant of P. gingivalis virulence.
AB - Porphyromonas gingivalis is a periodontal pathogen whose fimbriae are classified into six genotypes based on the diversity of the fimA genes encoding each fimbria subunit. It was suggested that P. gingivalis strains with type II fimbriae were more virulent than type I strains. For the present study, we generated the mutants in which fimA was substituted with different genotypes to study virulence of type II fimbriae. Using plasmid vectors, fimA of ATCC33277 (type I strain) was substituted with type II fimA, and that of OMZ314 (type II strain) with type I fimA. The substitution of type I fimA with type II enhanced bacterial adhesion/invasion to epithelial cells, whereas substitution with type I fimA resulted in diminished efficiency. Following bacterial invasion, type II clones swiftly degraded cellular paxillin and focal adhesion kinase, and inhibited cellular migration, whereas type I clones and ΔfimA mutants did not. BIAcore analysis demonstrated that type II fimbriae possess greater adhesive abilities for their receptor α5β1-integrin than those of type I. In a mouse abscess model, the type II clones significantly induced serum IL-1β and IL-6, as well as other infectious symptoms. These results suggest that type II fimbriae are a critical determinant of P. gingivalis virulence.
UR - http://www.scopus.com/inward/record.url?scp=33846907396&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846907396&partnerID=8YFLogxK
U2 - 10.1111/j.1462-5822.2006.00825.x
DO - 10.1111/j.1462-5822.2006.00825.x
M3 - Article
C2 - 17081195
AN - SCOPUS:33846907396
VL - 9
SP - 753
EP - 765
JO - Cellular Microbiology
JF - Cellular Microbiology
SN - 1462-5814
IS - 3
ER -