Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype

Takahiro Kato, Shinji Kawai, Kazuhiko Nakano, Hiroaki Inaba, Masae Kuboniwa, Ichiro Nakagawa, Kayoko Tsuda, Hiroko Omori, Takashi Ooshima, Tamotsu Yoshimori, Atsuo Amano

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Porphyromonas gingivalis is a periodontal pathogen whose fimbriae are classified into six genotypes based on the diversity of the fimA genes encoding each fimbria subunit. It was suggested that P. gingivalis strains with type II fimbriae were more virulent than type I strains. For the present study, we generated the mutants in which fimA was substituted with different genotypes to study virulence of type II fimbriae. Using plasmid vectors, fimA of ATCC33277 (type I strain) was substituted with type II fimA, and that of OMZ314 (type II strain) with type I fimA. The substitution of type I fimA with type II enhanced bacterial adhesion/invasion to epithelial cells, whereas substitution with type I fimA resulted in diminished efficiency. Following bacterial invasion, type II clones swiftly degraded cellular paxillin and focal adhesion kinase, and inhibited cellular migration, whereas type I clones and ΔfimA mutants did not. BIAcore analysis demonstrated that type II fimbriae possess greater adhesive abilities for their receptor α5β1-integrin than those of type I. In a mouse abscess model, the type II clones significantly induced serum IL-1β and IL-6, as well as other infectious symptoms. These results suggest that type II fimbriae are a critical determinant of P. gingivalis virulence.

Original languageEnglish
Pages (from-to)753-765
Number of pages13
JournalCellular Microbiology
Volume9
Issue number3
DOIs
Publication statusPublished - Mar 2007
Externally publishedYes

Fingerprint

Porphyromonas gingivalis
Virulence
Substitution reactions
Clone Cells
Genes
Genotype
Paxillin
Bacterial Adhesion
Focal Adhesion Protein-Tyrosine Kinases
Interleukin-1
Integrins
Adhesives
Abscess
Gene encoding
Interleukin-6
Plasmids
Pathogens
Epithelial Cells
Adhesion
Serum

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Microbiology

Cite this

Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype. / Kato, Takahiro; Kawai, Shinji; Nakano, Kazuhiko; Inaba, Hiroaki; Kuboniwa, Masae; Nakagawa, Ichiro; Tsuda, Kayoko; Omori, Hiroko; Ooshima, Takashi; Yoshimori, Tamotsu; Amano, Atsuo.

In: Cellular Microbiology, Vol. 9, No. 3, 03.2007, p. 753-765.

Research output: Contribution to journalArticle

Kato, T, Kawai, S, Nakano, K, Inaba, H, Kuboniwa, M, Nakagawa, I, Tsuda, K, Omori, H, Ooshima, T, Yoshimori, T & Amano, A 2007, 'Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype', Cellular Microbiology, vol. 9, no. 3, pp. 753-765. https://doi.org/10.1111/j.1462-5822.2006.00825.x
Kato, Takahiro ; Kawai, Shinji ; Nakano, Kazuhiko ; Inaba, Hiroaki ; Kuboniwa, Masae ; Nakagawa, Ichiro ; Tsuda, Kayoko ; Omori, Hiroko ; Ooshima, Takashi ; Yoshimori, Tamotsu ; Amano, Atsuo. / Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype. In: Cellular Microbiology. 2007 ; Vol. 9, No. 3. pp. 753-765.
@article{5bdfbe701b894f02816bda2b47b3c981,
title = "Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype",
abstract = "Porphyromonas gingivalis is a periodontal pathogen whose fimbriae are classified into six genotypes based on the diversity of the fimA genes encoding each fimbria subunit. It was suggested that P. gingivalis strains with type II fimbriae were more virulent than type I strains. For the present study, we generated the mutants in which fimA was substituted with different genotypes to study virulence of type II fimbriae. Using plasmid vectors, fimA of ATCC33277 (type I strain) was substituted with type II fimA, and that of OMZ314 (type II strain) with type I fimA. The substitution of type I fimA with type II enhanced bacterial adhesion/invasion to epithelial cells, whereas substitution with type I fimA resulted in diminished efficiency. Following bacterial invasion, type II clones swiftly degraded cellular paxillin and focal adhesion kinase, and inhibited cellular migration, whereas type I clones and ΔfimA mutants did not. BIAcore analysis demonstrated that type II fimbriae possess greater adhesive abilities for their receptor α5β1-integrin than those of type I. In a mouse abscess model, the type II clones significantly induced serum IL-1β and IL-6, as well as other infectious symptoms. These results suggest that type II fimbriae are a critical determinant of P. gingivalis virulence.",
author = "Takahiro Kato and Shinji Kawai and Kazuhiko Nakano and Hiroaki Inaba and Masae Kuboniwa and Ichiro Nakagawa and Kayoko Tsuda and Hiroko Omori and Takashi Ooshima and Tamotsu Yoshimori and Atsuo Amano",
year = "2007",
month = "3",
doi = "10.1111/j.1462-5822.2006.00825.x",
language = "English",
volume = "9",
pages = "753--765",
journal = "Cellular Microbiology",
issn = "1462-5814",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype

AU - Kato, Takahiro

AU - Kawai, Shinji

AU - Nakano, Kazuhiko

AU - Inaba, Hiroaki

AU - Kuboniwa, Masae

AU - Nakagawa, Ichiro

AU - Tsuda, Kayoko

AU - Omori, Hiroko

AU - Ooshima, Takashi

AU - Yoshimori, Tamotsu

AU - Amano, Atsuo

PY - 2007/3

Y1 - 2007/3

N2 - Porphyromonas gingivalis is a periodontal pathogen whose fimbriae are classified into six genotypes based on the diversity of the fimA genes encoding each fimbria subunit. It was suggested that P. gingivalis strains with type II fimbriae were more virulent than type I strains. For the present study, we generated the mutants in which fimA was substituted with different genotypes to study virulence of type II fimbriae. Using plasmid vectors, fimA of ATCC33277 (type I strain) was substituted with type II fimA, and that of OMZ314 (type II strain) with type I fimA. The substitution of type I fimA with type II enhanced bacterial adhesion/invasion to epithelial cells, whereas substitution with type I fimA resulted in diminished efficiency. Following bacterial invasion, type II clones swiftly degraded cellular paxillin and focal adhesion kinase, and inhibited cellular migration, whereas type I clones and ΔfimA mutants did not. BIAcore analysis demonstrated that type II fimbriae possess greater adhesive abilities for their receptor α5β1-integrin than those of type I. In a mouse abscess model, the type II clones significantly induced serum IL-1β and IL-6, as well as other infectious symptoms. These results suggest that type II fimbriae are a critical determinant of P. gingivalis virulence.

AB - Porphyromonas gingivalis is a periodontal pathogen whose fimbriae are classified into six genotypes based on the diversity of the fimA genes encoding each fimbria subunit. It was suggested that P. gingivalis strains with type II fimbriae were more virulent than type I strains. For the present study, we generated the mutants in which fimA was substituted with different genotypes to study virulence of type II fimbriae. Using plasmid vectors, fimA of ATCC33277 (type I strain) was substituted with type II fimA, and that of OMZ314 (type II strain) with type I fimA. The substitution of type I fimA with type II enhanced bacterial adhesion/invasion to epithelial cells, whereas substitution with type I fimA resulted in diminished efficiency. Following bacterial invasion, type II clones swiftly degraded cellular paxillin and focal adhesion kinase, and inhibited cellular migration, whereas type I clones and ΔfimA mutants did not. BIAcore analysis demonstrated that type II fimbriae possess greater adhesive abilities for their receptor α5β1-integrin than those of type I. In a mouse abscess model, the type II clones significantly induced serum IL-1β and IL-6, as well as other infectious symptoms. These results suggest that type II fimbriae are a critical determinant of P. gingivalis virulence.

UR - http://www.scopus.com/inward/record.url?scp=33846907396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846907396&partnerID=8YFLogxK

U2 - 10.1111/j.1462-5822.2006.00825.x

DO - 10.1111/j.1462-5822.2006.00825.x

M3 - Article

VL - 9

SP - 753

EP - 765

JO - Cellular Microbiology

JF - Cellular Microbiology

SN - 1462-5814

IS - 3

ER -