Viroplasm matrix protein Pns9 from rice gall dwarf virus forms an octameric cylindrical structure

Fusamichi Akita, Naoyuki Miyazaki, Hiroyuki Hibino, Takumi Shimizu, Akifumi Higashiura, Tamaki Uehara-Ichiki, Takahide Sasaya, Tomitake Tsukihara, Atsushi Nakagawa, Kenji Iwasaki, Toshihiro Omura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The non-structural Pns9 protein of rice gall dwarf virus (RGDV) accumulates in viroplasm inclusions, which are structures that appear to play an important role in viral morphogenesis and are commonly found in host cells infected by viruses in the family Reoviridae. Immunofluorescence and immunoelectron microscopy of RGDV-infected vector cells in monolayers, using antibodies against Pns9 of RGDV and expression of Pns9 in Spodoptera frugiperda cells, demonstrated that Pns9 is the minimal viral factor necessary for formation of viroplasm inclusion during infection by RGDV. When Pns9 in solution was observed under a conventional electron microscope, it appeared as ring-like aggregates of approximately 100 Å in diameter. Cryo-electron microscopic analysis of these aggregates revealed cylinders of octameric Pns9, whose dimensions were similar to those observed under the conventional electron microscope. Octamerization of Pns9 in solution was confirmed by the results of size-exclusion chromatography. Among proteins of viruses that belong to the family Reoviridae whose three-dimensional structures are available, a matrix protein of the viroplasm of rotavirus, NSP2, forms similar octamers, an observation that suggests similar roles for Pns9 and NSP2 in morphogenesis in animal-infecting and in plantinfecting reoviruses.

Original languageEnglish
Pages (from-to)2214-2221
Number of pages8
JournalJournal of General Virology
Volume92
Issue number9
DOIs
Publication statusPublished - Sep 2011
Externally publishedYes

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Viruses
Reoviridae
Proteins
Electrons
Morphogenesis
Spodoptera
Immunoelectron Microscopy
Fluorescence Microscopy
Gel Chromatography
Oryza
Observation
Antibodies
Infection

ASJC Scopus subject areas

  • Virology

Cite this

Akita, F., Miyazaki, N., Hibino, H., Shimizu, T., Higashiura, A., Uehara-Ichiki, T., ... Omura, T. (2011). Viroplasm matrix protein Pns9 from rice gall dwarf virus forms an octameric cylindrical structure. Journal of General Virology, 92(9), 2214-2221. https://doi.org/10.1099/vir.0.032524-0

Viroplasm matrix protein Pns9 from rice gall dwarf virus forms an octameric cylindrical structure. / Akita, Fusamichi; Miyazaki, Naoyuki; Hibino, Hiroyuki; Shimizu, Takumi; Higashiura, Akifumi; Uehara-Ichiki, Tamaki; Sasaya, Takahide; Tsukihara, Tomitake; Nakagawa, Atsushi; Iwasaki, Kenji; Omura, Toshihiro.

In: Journal of General Virology, Vol. 92, No. 9, 09.2011, p. 2214-2221.

Research output: Contribution to journalArticle

Akita, F, Miyazaki, N, Hibino, H, Shimizu, T, Higashiura, A, Uehara-Ichiki, T, Sasaya, T, Tsukihara, T, Nakagawa, A, Iwasaki, K & Omura, T 2011, 'Viroplasm matrix protein Pns9 from rice gall dwarf virus forms an octameric cylindrical structure', Journal of General Virology, vol. 92, no. 9, pp. 2214-2221. https://doi.org/10.1099/vir.0.032524-0
Akita, Fusamichi ; Miyazaki, Naoyuki ; Hibino, Hiroyuki ; Shimizu, Takumi ; Higashiura, Akifumi ; Uehara-Ichiki, Tamaki ; Sasaya, Takahide ; Tsukihara, Tomitake ; Nakagawa, Atsushi ; Iwasaki, Kenji ; Omura, Toshihiro. / Viroplasm matrix protein Pns9 from rice gall dwarf virus forms an octameric cylindrical structure. In: Journal of General Virology. 2011 ; Vol. 92, No. 9. pp. 2214-2221.
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