Virome capture sequencing does not identify active viral infection in unicentric and idiopathic multicentric Castleman disease

Christopher S. Nabel, Stephen Sameroff, Dustin Shilling, Daisy Alapat, Jason R. Ruth, Mitsuhiro Kawano, Yasuharu Sato, Katie Stone, Signe Spetalen, Federico Valdivieso, Michael D. Feldman, Amy Chadburn, Alexander Fosså, Frits van Rhee, W. Ian Lipkin, David C. Fajgenbaum

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Castleman disease (CD) describes a spectrum of heterogeneous disorders defined by characteristic lymph node histopathology. Enlarged lymph nodes demonstrating CD histopathology can occur in isolation (unicentric CD; UCD) sometimes accompanied by mild symptoms, or at multiple sites (multicentric CD, MCD) with systemic inflammation and cytokine-driven multi-organ dysfunction. The discovery that Kaposi sarcoma herpesvirus/ human herpesvirus (HHV)-8 drives MCD in a subset of patients has led to the hypotheses that UCD and MCD patients with negative HHV-8 testing by conventional methods may represent false negatives, or that these cases are driven by another virus, known or unknown. To investigate these hypotheses, the virome capture sequencing for vertebrate viruses (Vir-CapSeq-VERT) platform was employed to detect RNA transcripts from known and novel viruses in fresh frozen lymph node tissue from CD patients (12 UCD, 11 HHV-8-negative MCD [idiopathic MCD; iMCD], and two HHV-8-positive MCD) and related diseases (three T cell lymphoma and three Hodgkin lymphoma). This assay detected HHV-8 in both HHV-8positive cases; however, HHV-8 was not found in clinically HHV-8-negative iMCD or UCD cases. Additionally, no novel viruses were discovered, and no single known virus was detected with apparent association to HHV-8-negative CD cases. Herpesviridae family members, notably including Epstein-Barr virus (EBV), were detected in 7 out of 12 UCD and 5 of 11 iMCD cases with apparent correlations with markers of disease severity in iMCD. Analysis of a separate cohort of archival formalin-fixed, paraffin-embedded lymph node tissue by In situ hybridization revealed significantly fewer EBV-positive cells in UCD and iMCD compared to tissue from HHV-8-positive MCD and EBV-associated lymphoproliferative disorder. In an additional cohort, quantitative testing for EBV by PCR in peripheral blood during disease flare did not detect systemic EBV viremia, suggesting detection lymph node tissue is due to occult, local reactivation in UCD and iMCD. This study confirms that HHV-8 is not present in UCD and iMCD patients. Further, it fails to establish a clear association between any single virus, novel or known, and CD in HHV-8-negative cases. Given that distinct forms of CD exist with viral and non-viral etiological drivers, CD should be considered a group of distinct and separate diseases with heterogeneous causes worthy of further study.

Original languageEnglish
Article numbere0218660
JournalPloS one
Volume14
Issue number6
DOIs
Publication statusPublished - Jan 1 2019

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Human herpesvirus 8
Human Herpesvirus 8
Virus Diseases
Giant Lymph Node Hyperplasia
Viruses
Human herpesvirus 4
infection
Human Herpesvirus 4
lymph nodes
Lymph Nodes
Herpesviridae
viruses
Tissue
histopathology
Multi-centric Castleman's Disease
Hodgkin disease
hematologic diseases
vertebrate viruses
lymphatic diseases
Association reactions

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Nabel, C. S., Sameroff, S., Shilling, D., Alapat, D., Ruth, J. R., Kawano, M., ... Fajgenbaum, D. C. (2019). Virome capture sequencing does not identify active viral infection in unicentric and idiopathic multicentric Castleman disease. PloS one, 14(6), [e0218660]. https://doi.org/10.1371/journal.pone.0218660

Virome capture sequencing does not identify active viral infection in unicentric and idiopathic multicentric Castleman disease. / Nabel, Christopher S.; Sameroff, Stephen; Shilling, Dustin; Alapat, Daisy; Ruth, Jason R.; Kawano, Mitsuhiro; Sato, Yasuharu; Stone, Katie; Spetalen, Signe; Valdivieso, Federico; Feldman, Michael D.; Chadburn, Amy; Fosså, Alexander; van Rhee, Frits; Ian Lipkin, W.; Fajgenbaum, David C.

In: PloS one, Vol. 14, No. 6, e0218660, 01.01.2019.

Research output: Contribution to journalArticle

Nabel, CS, Sameroff, S, Shilling, D, Alapat, D, Ruth, JR, Kawano, M, Sato, Y, Stone, K, Spetalen, S, Valdivieso, F, Feldman, MD, Chadburn, A, Fosså, A, van Rhee, F, Ian Lipkin, W & Fajgenbaum, DC 2019, 'Virome capture sequencing does not identify active viral infection in unicentric and idiopathic multicentric Castleman disease', PloS one, vol. 14, no. 6, e0218660. https://doi.org/10.1371/journal.pone.0218660
Nabel, Christopher S. ; Sameroff, Stephen ; Shilling, Dustin ; Alapat, Daisy ; Ruth, Jason R. ; Kawano, Mitsuhiro ; Sato, Yasuharu ; Stone, Katie ; Spetalen, Signe ; Valdivieso, Federico ; Feldman, Michael D. ; Chadburn, Amy ; Fosså, Alexander ; van Rhee, Frits ; Ian Lipkin, W. ; Fajgenbaum, David C. / Virome capture sequencing does not identify active viral infection in unicentric and idiopathic multicentric Castleman disease. In: PloS one. 2019 ; Vol. 14, No. 6.
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abstract = "Castleman disease (CD) describes a spectrum of heterogeneous disorders defined by characteristic lymph node histopathology. Enlarged lymph nodes demonstrating CD histopathology can occur in isolation (unicentric CD; UCD) sometimes accompanied by mild symptoms, or at multiple sites (multicentric CD, MCD) with systemic inflammation and cytokine-driven multi-organ dysfunction. The discovery that Kaposi sarcoma herpesvirus/ human herpesvirus (HHV)-8 drives MCD in a subset of patients has led to the hypotheses that UCD and MCD patients with negative HHV-8 testing by conventional methods may represent false negatives, or that these cases are driven by another virus, known or unknown. To investigate these hypotheses, the virome capture sequencing for vertebrate viruses (Vir-CapSeq-VERT) platform was employed to detect RNA transcripts from known and novel viruses in fresh frozen lymph node tissue from CD patients (12 UCD, 11 HHV-8-negative MCD [idiopathic MCD; iMCD], and two HHV-8-positive MCD) and related diseases (three T cell lymphoma and three Hodgkin lymphoma). This assay detected HHV-8 in both HHV-8positive cases; however, HHV-8 was not found in clinically HHV-8-negative iMCD or UCD cases. Additionally, no novel viruses were discovered, and no single known virus was detected with apparent association to HHV-8-negative CD cases. Herpesviridae family members, notably including Epstein-Barr virus (EBV), were detected in 7 out of 12 UCD and 5 of 11 iMCD cases with apparent correlations with markers of disease severity in iMCD. Analysis of a separate cohort of archival formalin-fixed, paraffin-embedded lymph node tissue by In situ hybridization revealed significantly fewer EBV-positive cells in UCD and iMCD compared to tissue from HHV-8-positive MCD and EBV-associated lymphoproliferative disorder. In an additional cohort, quantitative testing for EBV by PCR in peripheral blood during disease flare did not detect systemic EBV viremia, suggesting detection lymph node tissue is due to occult, local reactivation in UCD and iMCD. This study confirms that HHV-8 is not present in UCD and iMCD patients. Further, it fails to establish a clear association between any single virus, novel or known, and CD in HHV-8-negative cases. Given that distinct forms of CD exist with viral and non-viral etiological drivers, CD should be considered a group of distinct and separate diseases with heterogeneous causes worthy of further study.",
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AU - Kawano, Mitsuhiro

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AU - Valdivieso, Federico

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N2 - Castleman disease (CD) describes a spectrum of heterogeneous disorders defined by characteristic lymph node histopathology. Enlarged lymph nodes demonstrating CD histopathology can occur in isolation (unicentric CD; UCD) sometimes accompanied by mild symptoms, or at multiple sites (multicentric CD, MCD) with systemic inflammation and cytokine-driven multi-organ dysfunction. The discovery that Kaposi sarcoma herpesvirus/ human herpesvirus (HHV)-8 drives MCD in a subset of patients has led to the hypotheses that UCD and MCD patients with negative HHV-8 testing by conventional methods may represent false negatives, or that these cases are driven by another virus, known or unknown. To investigate these hypotheses, the virome capture sequencing for vertebrate viruses (Vir-CapSeq-VERT) platform was employed to detect RNA transcripts from known and novel viruses in fresh frozen lymph node tissue from CD patients (12 UCD, 11 HHV-8-negative MCD [idiopathic MCD; iMCD], and two HHV-8-positive MCD) and related diseases (three T cell lymphoma and three Hodgkin lymphoma). This assay detected HHV-8 in both HHV-8positive cases; however, HHV-8 was not found in clinically HHV-8-negative iMCD or UCD cases. Additionally, no novel viruses were discovered, and no single known virus was detected with apparent association to HHV-8-negative CD cases. Herpesviridae family members, notably including Epstein-Barr virus (EBV), were detected in 7 out of 12 UCD and 5 of 11 iMCD cases with apparent correlations with markers of disease severity in iMCD. Analysis of a separate cohort of archival formalin-fixed, paraffin-embedded lymph node tissue by In situ hybridization revealed significantly fewer EBV-positive cells in UCD and iMCD compared to tissue from HHV-8-positive MCD and EBV-associated lymphoproliferative disorder. In an additional cohort, quantitative testing for EBV by PCR in peripheral blood during disease flare did not detect systemic EBV viremia, suggesting detection lymph node tissue is due to occult, local reactivation in UCD and iMCD. This study confirms that HHV-8 is not present in UCD and iMCD patients. Further, it fails to establish a clear association between any single virus, novel or known, and CD in HHV-8-negative cases. Given that distinct forms of CD exist with viral and non-viral etiological drivers, CD should be considered a group of distinct and separate diseases with heterogeneous causes worthy of further study.

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