Vibrio mimicus attaches to the intestinal mucosa by outer membrane hemagglutinins specific to polypeptide moieties of glycoproteins

Munirul Alam, Shin-ichi Miyoshi, Ken Ichi Tomochika, Sumio Shinoda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Vibrio mimicus is the closest organism to Fibrin cholerae. V. mimicus E-33, which is a highly adhesive and enteropathogenic strain, is known to produce three types of hemagglutinins (HAs), i.e., a 31-kDa exocellular metalloprotease (Vm-HA/protease), lipopolysaccharide (Vm-LPSHA), and a 39-kDa major outer membrane protein (Vm-OMPHA). Hemagglutination induced by Vm-LPSHA and Vm- OMPHA was inhibited by glycoproteins, including mucin, fetuin, and asialofetuin, but not by monosaccharides, disaccharides, or N- acetylated saccharides. The inhibitory potential of each glycoprotein for Vm-OMPHA was greatly augmented by treatment with a glycolytic enzyme such as β-D-galactosidase or β-D-glucosidase, while pronase treatment achieved complete abolition of the inhibitory potential. The inhibitory ability of the glycoproteins for Vm-LPSHA was also abolished by pronase treatment; however, glycolytic enzyme treatment showed no effect. Hence, the polypeptide portion of glycoproteins may directly associate with Vm-OMPHA and Vm-LPSHA, but the sugar moiety may act as a barrier to interaction with Vm-OMPHA. The glycoproteins as well as Fab antibodies against Vm-OMPHA and Vm-LPSHA eliminated the ability of E-33 cells to agglutinate rabbit erythrocytes and to attach to rabbit intestinal mucosa. Additionally, expression of the hemagglutinating ability by the bacterial cells was accompanied by efficient bacterial adherence to the intestinal mucosa. Finally, the hemagglutinating activity of Vm-OMPHA was markedly increased by incubation with Vm-HA/protease. These results indicate that all three HAs may have significant roles in the glycoprotein-mediated intestinal adherence of V. mimicus E-33.

Original languageEnglish
Pages (from-to)3662-3665
Number of pages4
JournalInfection and Immunity
Volume65
Issue number9
Publication statusPublished - Sep 1997

Fingerprint

Vibrio mimicus
Hemagglutinins
Intestinal Mucosa
Glycoproteins
Lipopolysaccharides
Peptides
Membranes
Pronase
Galactosidases
Fetuins
Glucosidases
Rabbits
Monosaccharides
Disaccharides
Cholera
Hemagglutination
Metalloproteases
Mucins
Enzymes
Fibrin

ASJC Scopus subject areas

  • Immunology

Cite this

Vibrio mimicus attaches to the intestinal mucosa by outer membrane hemagglutinins specific to polypeptide moieties of glycoproteins. / Alam, Munirul; Miyoshi, Shin-ichi; Tomochika, Ken Ichi; Shinoda, Sumio.

In: Infection and Immunity, Vol. 65, No. 9, 09.1997, p. 3662-3665.

Research output: Contribution to journalArticle

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