Vibrio mimicus are the reservoirs of the heat-stable enterotoxin gene (nag-st) among species of the genus Vibrio

P. Yuan, A. Ogawa, T. Ramamurthy, G. B. Nair, T. Shimada, S. Shinoda, T. Takeda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Using a 0.27 kb DNA probe specific for the heat-stable enterotoxin gene (nag-st) of Vibrio cholerae non-O1, 1109 strains representing 17 species of the genus Vibrio, isolated from clinical and environmental sources were examined. The nag-st gene was preponderantly associated with strains classified as V. mimicus; 16.8% of these strains hybridized. It was more frequent in the clinical isolates (22.6%) than in the environmental isolates (13.7%). The incidence of nag-st gene-positive strains of V. mimicus isolated from different countries was uniformly high and ranged between 8.7% (Bangladesh) and 57.1% (environmental strains from USA). The incidence of the nag-st gene was much lower among strains of V. cholerae non-O1 (3.6%). Probe-positive and-negative strains of V. mimicus and V. cholerae non-O1 were used to evaluate the performance of the conventional suckling mouse assay for detection of the NAG-ST enterotoxin. Of the 31 probe-positive strains, only five (16.1%) yielded a positive fluid accumulation ratio (FA ratio) when neat heated culture supernatant was used to perform the suckling mouse assay. All the 31 probe-positive strains gave a positive FA ratio when 20-fold concentrated and heated culture supernatants of the strains were used to perform the suckling mouse assay. The need to concentrate (by at least 20-fold) the culture supernatant of strains of V. mimicus and V. Cholerae non-O1 was identified as an important step to obtain consistent results when using the suckling mouse assay for detection of NAG-ST.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalWorld Journal of Microbiology and Biotechnology
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1994
Externally publishedYes

Fingerprint

Vibrio mimicus enterotoxin
Vibrio mimicus
Vibrio
Vibrio cholerae non-O1
enterotoxins
Genes
heat
Assays
genes
suckling
Bangladesh
Cholera
Enterotoxins
Incidence
DNA Probes
mice
Fluids
assays
Hot Temperature
angle of incidence

Keywords

  • DNA probe
  • heat-stable enterotoxin
  • NAG-ST
  • Vibrio
  • Vibrio mimicus

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Biochemistry
  • Plant Science
  • Food Science

Cite this

Vibrio mimicus are the reservoirs of the heat-stable enterotoxin gene (nag-st) among species of the genus Vibrio. / Yuan, P.; Ogawa, A.; Ramamurthy, T.; Nair, G. B.; Shimada, T.; Shinoda, S.; Takeda, T.

In: World Journal of Microbiology and Biotechnology, Vol. 10, No. 1, 01.1994, p. 59-63.

Research output: Contribution to journalArticle

Yuan, P. ; Ogawa, A. ; Ramamurthy, T. ; Nair, G. B. ; Shimada, T. ; Shinoda, S. ; Takeda, T. / Vibrio mimicus are the reservoirs of the heat-stable enterotoxin gene (nag-st) among species of the genus Vibrio. In: World Journal of Microbiology and Biotechnology. 1994 ; Vol. 10, No. 1. pp. 59-63.
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AU - Nair, G. B.

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AU - Shinoda, S.

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AB - Using a 0.27 kb DNA probe specific for the heat-stable enterotoxin gene (nag-st) of Vibrio cholerae non-O1, 1109 strains representing 17 species of the genus Vibrio, isolated from clinical and environmental sources were examined. The nag-st gene was preponderantly associated with strains classified as V. mimicus; 16.8% of these strains hybridized. It was more frequent in the clinical isolates (22.6%) than in the environmental isolates (13.7%). The incidence of nag-st gene-positive strains of V. mimicus isolated from different countries was uniformly high and ranged between 8.7% (Bangladesh) and 57.1% (environmental strains from USA). The incidence of the nag-st gene was much lower among strains of V. cholerae non-O1 (3.6%). Probe-positive and-negative strains of V. mimicus and V. cholerae non-O1 were used to evaluate the performance of the conventional suckling mouse assay for detection of the NAG-ST enterotoxin. Of the 31 probe-positive strains, only five (16.1%) yielded a positive fluid accumulation ratio (FA ratio) when neat heated culture supernatant was used to perform the suckling mouse assay. All the 31 probe-positive strains gave a positive FA ratio when 20-fold concentrated and heated culture supernatants of the strains were used to perform the suckling mouse assay. The need to concentrate (by at least 20-fold) the culture supernatant of strains of V. mimicus and V. Cholerae non-O1 was identified as an important step to obtain consistent results when using the suckling mouse assay for detection of NAG-ST.

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