Viability of barley seeds after long-term exposure to outer side of international space station

Manabu Sugimoto, Makoto Ishii, Izumi Mori, Shagimardanova Elena, Oleg A. Gusev, Makoto Kihara, Takehiro Hoki, Vladimir N. Sychev, Margarita A. Levinskikh, Natalia D. Novikova, Anatoly I. Grigoriev

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Barley seeds were exposed to outer space for 13 months in a vented metal container without a climate control system to assess the risk of physiological and genetic mutation during long-term storage in space. The space-stored seeds (S0 generation), with an 82% germination rate in 50 seeds, lost about 20% of their weight after the exposure. The germinated seeds showed normal growth, heading, and ripening. The harvested seeds (S1 generation) also germinated and reproduced (S2 generation) as did the ground-stored seeds. The culm length, ear length, number of seed, grain weight, and fertility of the plants from the space-stored seeds were not significantly different from those of the ground-stored seeds in each of the S0 and S1 generation. Furthermore, the S1 and S2 space-stored seeds respectively showed similar β-glucan content to those of the ground-stored seeds. Amplified fragment length polymorphism analysis with 16 primer combinations showed no specific fragment that appears or disappears significantly in the DNA isolated from the barley grown from the space-stored seeds. Though these data are derived from nine S0 space-stored seeds in a single exposure experiment, the results demonstrate the preservation of barley seeds in outer space for 13 months without phenotypic or genotypic changes and with healthy and vigorous growth in space.

Original languageEnglish
Pages (from-to)1155-1160
Number of pages6
JournalAdvances in Space Research
Volume48
Issue number6
DOIs
Publication statusPublished - Sep 15 2011

Fingerprint

barley
International Space Station
Space stations
viability
Seed
seeds
seed
station
exposure
fragments
Climate control
fertility
germination
primers
ripening
polymorphism
ear
mutations

Keywords

  • Barley
  • International space station
  • Outer space
  • Seed storage
  • Space radiation

ASJC Scopus subject areas

  • Aerospace Engineering
  • Space and Planetary Science

Cite this

Viability of barley seeds after long-term exposure to outer side of international space station. / Sugimoto, Manabu; Ishii, Makoto; Mori, Izumi; Elena, Shagimardanova; Gusev, Oleg A.; Kihara, Makoto; Hoki, Takehiro; Sychev, Vladimir N.; Levinskikh, Margarita A.; Novikova, Natalia D.; Grigoriev, Anatoly I.

In: Advances in Space Research, Vol. 48, No. 6, 15.09.2011, p. 1155-1160.

Research output: Contribution to journalArticle

Sugimoto, M, Ishii, M, Mori, I, Elena, S, Gusev, OA, Kihara, M, Hoki, T, Sychev, VN, Levinskikh, MA, Novikova, ND & Grigoriev, AI 2011, 'Viability of barley seeds after long-term exposure to outer side of international space station', Advances in Space Research, vol. 48, no. 6, pp. 1155-1160. https://doi.org/10.1016/j.asr.2011.05.017
Sugimoto, Manabu ; Ishii, Makoto ; Mori, Izumi ; Elena, Shagimardanova ; Gusev, Oleg A. ; Kihara, Makoto ; Hoki, Takehiro ; Sychev, Vladimir N. ; Levinskikh, Margarita A. ; Novikova, Natalia D. ; Grigoriev, Anatoly I. / Viability of barley seeds after long-term exposure to outer side of international space station. In: Advances in Space Research. 2011 ; Vol. 48, No. 6. pp. 1155-1160.
@article{6293b79fa3f2494fb890758a6b3a2ce6,
title = "Viability of barley seeds after long-term exposure to outer side of international space station",
abstract = "Barley seeds were exposed to outer space for 13 months in a vented metal container without a climate control system to assess the risk of physiological and genetic mutation during long-term storage in space. The space-stored seeds (S0 generation), with an 82{\%} germination rate in 50 seeds, lost about 20{\%} of their weight after the exposure. The germinated seeds showed normal growth, heading, and ripening. The harvested seeds (S1 generation) also germinated and reproduced (S2 generation) as did the ground-stored seeds. The culm length, ear length, number of seed, grain weight, and fertility of the plants from the space-stored seeds were not significantly different from those of the ground-stored seeds in each of the S0 and S1 generation. Furthermore, the S1 and S2 space-stored seeds respectively showed similar β-glucan content to those of the ground-stored seeds. Amplified fragment length polymorphism analysis with 16 primer combinations showed no specific fragment that appears or disappears significantly in the DNA isolated from the barley grown from the space-stored seeds. Though these data are derived from nine S0 space-stored seeds in a single exposure experiment, the results demonstrate the preservation of barley seeds in outer space for 13 months without phenotypic or genotypic changes and with healthy and vigorous growth in space.",
keywords = "Barley, International space station, Outer space, Seed storage, Space radiation",
author = "Manabu Sugimoto and Makoto Ishii and Izumi Mori and Shagimardanova Elena and Gusev, {Oleg A.} and Makoto Kihara and Takehiro Hoki and Sychev, {Vladimir N.} and Levinskikh, {Margarita A.} and Novikova, {Natalia D.} and Grigoriev, {Anatoly I.}",
year = "2011",
month = "9",
day = "15",
doi = "10.1016/j.asr.2011.05.017",
language = "English",
volume = "48",
pages = "1155--1160",
journal = "Advances in Space Research",
issn = "0273-1177",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Viability of barley seeds after long-term exposure to outer side of international space station

AU - Sugimoto, Manabu

AU - Ishii, Makoto

AU - Mori, Izumi

AU - Elena, Shagimardanova

AU - Gusev, Oleg A.

AU - Kihara, Makoto

AU - Hoki, Takehiro

AU - Sychev, Vladimir N.

AU - Levinskikh, Margarita A.

AU - Novikova, Natalia D.

AU - Grigoriev, Anatoly I.

PY - 2011/9/15

Y1 - 2011/9/15

N2 - Barley seeds were exposed to outer space for 13 months in a vented metal container without a climate control system to assess the risk of physiological and genetic mutation during long-term storage in space. The space-stored seeds (S0 generation), with an 82% germination rate in 50 seeds, lost about 20% of their weight after the exposure. The germinated seeds showed normal growth, heading, and ripening. The harvested seeds (S1 generation) also germinated and reproduced (S2 generation) as did the ground-stored seeds. The culm length, ear length, number of seed, grain weight, and fertility of the plants from the space-stored seeds were not significantly different from those of the ground-stored seeds in each of the S0 and S1 generation. Furthermore, the S1 and S2 space-stored seeds respectively showed similar β-glucan content to those of the ground-stored seeds. Amplified fragment length polymorphism analysis with 16 primer combinations showed no specific fragment that appears or disappears significantly in the DNA isolated from the barley grown from the space-stored seeds. Though these data are derived from nine S0 space-stored seeds in a single exposure experiment, the results demonstrate the preservation of barley seeds in outer space for 13 months without phenotypic or genotypic changes and with healthy and vigorous growth in space.

AB - Barley seeds were exposed to outer space for 13 months in a vented metal container without a climate control system to assess the risk of physiological and genetic mutation during long-term storage in space. The space-stored seeds (S0 generation), with an 82% germination rate in 50 seeds, lost about 20% of their weight after the exposure. The germinated seeds showed normal growth, heading, and ripening. The harvested seeds (S1 generation) also germinated and reproduced (S2 generation) as did the ground-stored seeds. The culm length, ear length, number of seed, grain weight, and fertility of the plants from the space-stored seeds were not significantly different from those of the ground-stored seeds in each of the S0 and S1 generation. Furthermore, the S1 and S2 space-stored seeds respectively showed similar β-glucan content to those of the ground-stored seeds. Amplified fragment length polymorphism analysis with 16 primer combinations showed no specific fragment that appears or disappears significantly in the DNA isolated from the barley grown from the space-stored seeds. Though these data are derived from nine S0 space-stored seeds in a single exposure experiment, the results demonstrate the preservation of barley seeds in outer space for 13 months without phenotypic or genotypic changes and with healthy and vigorous growth in space.

KW - Barley

KW - International space station

KW - Outer space

KW - Seed storage

KW - Space radiation

UR - http://www.scopus.com/inward/record.url?scp=79960994562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960994562&partnerID=8YFLogxK

U2 - 10.1016/j.asr.2011.05.017

DO - 10.1016/j.asr.2011.05.017

M3 - Article

AN - SCOPUS:79960994562

VL - 48

SP - 1155

EP - 1160

JO - Advances in Space Research

JF - Advances in Space Research

SN - 0273-1177

IS - 6

ER -