Vesicular inhibitory amino acid transporter is a Cl-/ γ-aminobutyrate co-transporter

Narinobu Juge, Akiko Muroyama, Miki Hiasa, Hiroshi Omote, Yoshinori Moriyama

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The vesicular inhibitory amino acid transporter (VIAAT) is a synaptic vesicle protein responsible for the vesicular storage of γ-aminobutyrate (GABA) and glycine which plays an essential role in GABAergic and glycinergic neurotransmission. The transport mechanism of VIAAT remains largely unknown. Here, we show that proteoliposomes containing purified VIAAT actively took up GABA upon formation of membrane potential (Δψ) (positive inside) but not ΔpH. VIAAT-mediated GABA uptake had an absolute requirement for Cl- and actually accompanied Cl- movement. Kinetic analysis indicated that one GABA molecule and two Cl- equivalents were transported during one transport cycle. VIAAT in which Glu213 was specifically mutated to alanine completely lost the ability to take up both GABA and Cl-. Essentially the same results were obtained with glycine, another substrate of VIAAT. These results demonstrated that VIAAT is a vesicular Cl- transporter that co-transports Cl- with GABA or glycine in a Δψ dependent manner. It is concluded that Cl- plays an essential role in vesicular storage of GABA and glycine.

Original languageEnglish
Pages (from-to)35073-35078
Number of pages6
JournalJournal of Biological Chemistry
Volume284
Issue number50
DOIs
Publication statusPublished - Dec 11 2009

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Aminobutyrates
Symporters
Amino Acid Transport Systems
gamma-Aminobutyric Acid
Glycine
Synaptic Vesicles
Synaptic Transmission
Alanine
Membrane Potentials
Membranes
Molecules
Kinetics
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Vesicular inhibitory amino acid transporter is a Cl-/ γ-aminobutyrate co-transporter. / Juge, Narinobu; Muroyama, Akiko; Hiasa, Miki; Omote, Hiroshi; Moriyama, Yoshinori.

In: Journal of Biological Chemistry, Vol. 284, No. 50, 11.12.2009, p. 35073-35078.

Research output: Contribution to journalArticle

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