Vesicular GABA transporter (VGAT) transports β-alanine

Narinobu Juge, Hiroshi Omote, Yoshinori Moriyama

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9 Citations (Scopus)

Abstract

Vesicular GABA transporter (VGAT) is expressed in GABAergic and glycinergic neurons, and is responsible for vesicular storage and subsequent exocytosis of these inhibitory amino acids. In this study, we show that VGAT recognizes β-alanine as a substrate. Proteoliposomes containing purified VGAT transport β-alanine using Δψ but not ΔpH as a driving force. The Δψ-driven β-alanine uptake requires Cl-. VGAT also facilitates Cl- uptake in the presence of β-alanine. A previously described VGAT mutant (Glu213Ala) that disrupts GABA and glycine transport similarly abrogates β-alanine uptake. These findings indicated that VGAT transports β-alanine through a mechanism similar to those for GABA and glycine, and functions as a vesicular β-alanine transporter. Vesicular GABA transporter (VGAT) is expressed in GABAergic and glycinergic neurons, and is responsible for vesicular storage and subsequent exocytosis of these inhibitory amino acids. In the present study, we showed that proteoliposomes containing purified VGAT transport β-alanine using Δψ as a driving force. VGAT also facilitates Cl- uptake. Our findings indicated that VGAT functions as a vesicular β-alanine transporter. Vesicular GABA transporter (VGAT) is expressed in GABAergic and glycinergic neurons, and is responsible for vesicular storage and subsequent exocytosis of these inhibitory amino acids. In the present study, we showed that proteoliposomes containing purified VGAT transport β-alanine using Δψ as a driving force. VGAT also facilitates Cl- uptake. Our findings indicated that VGAT functions as a vesicular β-alanine transporter.

Original languageEnglish
Pages (from-to)482-486
Number of pages5
JournalJournal of Neurochemistry
Volume127
Issue number4
DOIs
Publication statusPublished - Nov 1 2013

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Keywords

  • taurine
  • vesicular GABA transporter
  • β-alanine

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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