Very strong UV-A light temporally separates the photoinhibition of photosystem II into light-induced inactivation and repair

Otto Zsiros, Suleyman Allakhverdiev, Shoichi Higashi, Masakatsu Watanabe, Yoshitaka Nishiyama, Norio Murata

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

When organisms that perform oxygenic photosynthesis are exposed to strong visible or UV light, inactivation of photosystem II (PSII) occurs. However, such organisms are able rapidly to repair the photoinactivated PSII. The phenomenon of photoinactivation and repair is known as photoinhibition. Under normal laboratory conditions, the rate of repair is similar to or faster than the rate of photoinactivation, preventing the detailed analysis of photoinactivation and repair as separate processes. We report here that, using strong UV-A light from a laser, we were able to analyze separately the photoinactivation and repair of photosystem II in the cyanobacterium Synechocystis sp. PCC 6803. Very strong UV-A light at 364 nm and a photon flux density of 2600 μmol photons m -2 s-1 inactivated the oxygen-evolving machinery and the photochemical reaction center of PSII within 1 or 2 min before the first step in the repair process, namely, the degradation of the D1 protein, occurred. During subsequent incubation of cells in weak visible light, the activity of PSII recovered fully within 30 min and this process depended on protein synthesis. During subsequent incubation of cells in darkness for 60 min, the D1 protein of the photoinactivated PSII was degraded. Further incubation in weak visible light resulted in the rapid restoration of the activity of PSII. These observations suggest that very strong UV-A light is a useful tool for the analysis of the repair of PSII after photoinactivation.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1757
Issue number2
DOIs
Publication statusPublished - Feb 1 2006
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
Ultraviolet Rays
Repair
Light
Photons
Cells
Synechocystis
Proteins
Photosynthesis
Photochemical reactions
Darkness
Cyanobacteria
Ultraviolet radiation
Proteolysis
Restoration
Machinery
Lasers
Oxygen
Fluxes
Degradation

Keywords

  • D1 protein
  • Photoinhibition
  • Photosystem II
  • Synechocystis
  • UV-A light

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Very strong UV-A light temporally separates the photoinhibition of photosystem II into light-induced inactivation and repair. / Zsiros, Otto; Allakhverdiev, Suleyman; Higashi, Shoichi; Watanabe, Masakatsu; Nishiyama, Yoshitaka; Murata, Norio.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1757, No. 2, 01.02.2006, p. 123-129.

Research output: Contribution to journalArticle

Zsiros, Otto ; Allakhverdiev, Suleyman ; Higashi, Shoichi ; Watanabe, Masakatsu ; Nishiyama, Yoshitaka ; Murata, Norio. / Very strong UV-A light temporally separates the photoinhibition of photosystem II into light-induced inactivation and repair. In: Biochimica et Biophysica Acta - Bioenergetics. 2006 ; Vol. 1757, No. 2. pp. 123-129.
@article{f20d0a52cb994f3bb76178c9f1737d42,
title = "Very strong UV-A light temporally separates the photoinhibition of photosystem II into light-induced inactivation and repair",
abstract = "When organisms that perform oxygenic photosynthesis are exposed to strong visible or UV light, inactivation of photosystem II (PSII) occurs. However, such organisms are able rapidly to repair the photoinactivated PSII. The phenomenon of photoinactivation and repair is known as photoinhibition. Under normal laboratory conditions, the rate of repair is similar to or faster than the rate of photoinactivation, preventing the detailed analysis of photoinactivation and repair as separate processes. We report here that, using strong UV-A light from a laser, we were able to analyze separately the photoinactivation and repair of photosystem II in the cyanobacterium Synechocystis sp. PCC 6803. Very strong UV-A light at 364 nm and a photon flux density of 2600 μmol photons m -2 s-1 inactivated the oxygen-evolving machinery and the photochemical reaction center of PSII within 1 or 2 min before the first step in the repair process, namely, the degradation of the D1 protein, occurred. During subsequent incubation of cells in weak visible light, the activity of PSII recovered fully within 30 min and this process depended on protein synthesis. During subsequent incubation of cells in darkness for 60 min, the D1 protein of the photoinactivated PSII was degraded. Further incubation in weak visible light resulted in the rapid restoration of the activity of PSII. These observations suggest that very strong UV-A light is a useful tool for the analysis of the repair of PSII after photoinactivation.",
keywords = "D1 protein, Photoinhibition, Photosystem II, Synechocystis, UV-A light",
author = "Otto Zsiros and Suleyman Allakhverdiev and Shoichi Higashi and Masakatsu Watanabe and Yoshitaka Nishiyama and Norio Murata",
year = "2006",
month = "2",
day = "1",
doi = "10.1016/j.bbabio.2006.01.004",
language = "English",
volume = "1757",
pages = "123--129",
journal = "Biochimica et Biophysica Acta - Bioenergetics",
issn = "0005-2728",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Very strong UV-A light temporally separates the photoinhibition of photosystem II into light-induced inactivation and repair

AU - Zsiros, Otto

AU - Allakhverdiev, Suleyman

AU - Higashi, Shoichi

AU - Watanabe, Masakatsu

AU - Nishiyama, Yoshitaka

AU - Murata, Norio

PY - 2006/2/1

Y1 - 2006/2/1

N2 - When organisms that perform oxygenic photosynthesis are exposed to strong visible or UV light, inactivation of photosystem II (PSII) occurs. However, such organisms are able rapidly to repair the photoinactivated PSII. The phenomenon of photoinactivation and repair is known as photoinhibition. Under normal laboratory conditions, the rate of repair is similar to or faster than the rate of photoinactivation, preventing the detailed analysis of photoinactivation and repair as separate processes. We report here that, using strong UV-A light from a laser, we were able to analyze separately the photoinactivation and repair of photosystem II in the cyanobacterium Synechocystis sp. PCC 6803. Very strong UV-A light at 364 nm and a photon flux density of 2600 μmol photons m -2 s-1 inactivated the oxygen-evolving machinery and the photochemical reaction center of PSII within 1 or 2 min before the first step in the repair process, namely, the degradation of the D1 protein, occurred. During subsequent incubation of cells in weak visible light, the activity of PSII recovered fully within 30 min and this process depended on protein synthesis. During subsequent incubation of cells in darkness for 60 min, the D1 protein of the photoinactivated PSII was degraded. Further incubation in weak visible light resulted in the rapid restoration of the activity of PSII. These observations suggest that very strong UV-A light is a useful tool for the analysis of the repair of PSII after photoinactivation.

AB - When organisms that perform oxygenic photosynthesis are exposed to strong visible or UV light, inactivation of photosystem II (PSII) occurs. However, such organisms are able rapidly to repair the photoinactivated PSII. The phenomenon of photoinactivation and repair is known as photoinhibition. Under normal laboratory conditions, the rate of repair is similar to or faster than the rate of photoinactivation, preventing the detailed analysis of photoinactivation and repair as separate processes. We report here that, using strong UV-A light from a laser, we were able to analyze separately the photoinactivation and repair of photosystem II in the cyanobacterium Synechocystis sp. PCC 6803. Very strong UV-A light at 364 nm and a photon flux density of 2600 μmol photons m -2 s-1 inactivated the oxygen-evolving machinery and the photochemical reaction center of PSII within 1 or 2 min before the first step in the repair process, namely, the degradation of the D1 protein, occurred. During subsequent incubation of cells in weak visible light, the activity of PSII recovered fully within 30 min and this process depended on protein synthesis. During subsequent incubation of cells in darkness for 60 min, the D1 protein of the photoinactivated PSII was degraded. Further incubation in weak visible light resulted in the rapid restoration of the activity of PSII. These observations suggest that very strong UV-A light is a useful tool for the analysis of the repair of PSII after photoinactivation.

KW - D1 protein

KW - Photoinhibition

KW - Photosystem II

KW - Synechocystis

KW - UV-A light

UR - http://www.scopus.com/inward/record.url?scp=33645013992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645013992&partnerID=8YFLogxK

U2 - 10.1016/j.bbabio.2006.01.004

DO - 10.1016/j.bbabio.2006.01.004

M3 - Article

C2 - 16500615

AN - SCOPUS:33645013992

VL - 1757

SP - 123

EP - 129

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

SN - 0005-2728

IS - 2

ER -