Vertebrate ancient-long opsin has molecular properties intermediate between those of vertebrate and invertebrate visual pigments

Keita Sato, Takahiro Yamashita, Hideyo Ohuchi, Yoshinori Shichida

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

VA/VAL opsin is one of the four kinds of nonvisual opsins that are closely related to vertebrate visual pigments in the phylogenetic tree of opsins. Previous studies indicated that among these opsins, parapinopsin and pinopsin exhibit molecular properties similar to those of invertebrate bistable visual pigments and vertebrate visual pigments, respectively. Here we show that VA/VAL opsin exhibits molecular properties intermediate between those of parapinopsin and pinopsin. VAL opsin from Xenopus tropicalis was expressed in cultured cells, and the pigment with an absorption maximum at 501 nm was reconstituted by incubation with 11-cis-retinal. Light irradiation of this pigment caused cis-to-trans isomerization of the chromophore to form a state having an absorption maximum in the visible region. This state has the ability to activate Gi and Gt types of G proteins. Therefore, the active state of VAL opsin is a visible light-absorbing intermediate, which probably has a protonated retinylidene Schiff base as its chromophore, like the active state of parapinopsin. However, this state was apparently photoinsensitive and did not show reverse reaction to the original pigment, unlike the active state of parapinopsin, and instead similar to that of pinopsin. Furthermore, the Gi activation efficiency of VAL opsin was between those of pinopsin and parapinopsin. Thus, the molecular properties of VA/VAL opsin give insights into the mechanism of conversion of the molecular properties from invertebrate to vertebrate visual pigments.

Original languageEnglish
Pages (from-to)10484-10490
Number of pages7
JournalBiochemistry
Volume50
Issue number48
DOIs
Publication statusPublished - Dec 6 2011
Externally publishedYes

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Opsins
Retinal Pigments
Invertebrates
Vertebrates
Pigments
Chromophores
Retinaldehyde
Light
Schiff Bases
Isomerization
Xenopus
GTP-Binding Proteins
Cultured Cells
Chemical activation
Cells
Irradiation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Vertebrate ancient-long opsin has molecular properties intermediate between those of vertebrate and invertebrate visual pigments. / Sato, Keita; Yamashita, Takahiro; Ohuchi, Hideyo; Shichida, Yoshinori.

In: Biochemistry, Vol. 50, No. 48, 06.12.2011, p. 10484-10490.

Research output: Contribution to journalArticle

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