Versican is induced in infiltrating monocytes in myocardial infarction

Kenichi Toeda, Keigo Nakamura, Satoshi Hirohata, Omer F. Hatipoglu, Kadir Demircan, Hitoshi Yamawaki, Hiroko Ogawa, Shozo Kusachi, Yasushi Shiratori, Yoshifumi Ninomiya

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Versican, a large chondroitin sulfate proteoglycan, plays a role in conditions such as wound healing and tissue remodelling. To test the hypothesis that versican expression is transiently upregulated and plays a role in the infarcted heart, we examined its expression in a rat model of myocardial infarction. Northern blot analysis demonstrated increased expression of versican mRNA. Quantitative real-time RT-PCR analysis revealed that versican mRNA began to increase as early as 6 h and reached its maximal level 2 days after coronary artery ligation. Versican mRNA then gradually decreased, while the mRNA of decorin, another small proteoglycan, increased thereafter. Versican mRNA was localized in monocytes, as indicated by CD68-positive staining, around the infarct tissue. The induction of versican mRNA was accelerated by ischemia/reperfusion (I/R), which was characterized by massive cell infiltration and enhanced inflammatory response. To examine the alteration of versican expression in monocytes/macrophages, we isolated human peripheral blood mononuclear cells and stimulated them with granulocyte/macrophage colony-stimulating factor (GM-CSF). Stimulation of mononuclear cells with GM-CSF increased the expression of versican mRNA as well as cytokine induction. The production of versican by monocytes in the infarct area represents a novel finding of the expression of an extracellular matrix gene by monocytes in the infarcted heart. We suggest that upregulation of versican in the infarcted myocardium may have a role in the inflammatory reaction, which mediates subsequent chemotaxis in the infarcted heart.

Original languageEnglish
Pages (from-to)47-56
Number of pages10
JournalMolecular and Cellular Biochemistry
Volume280
Issue number1-2
DOIs
Publication statusPublished - Dec 2005

Fingerprint

Versicans
Monocytes
Myocardial Infarction
Messenger RNA
Granulocyte-Macrophage Colony-Stimulating Factor
Tissue
Decorin
Chondroitin Sulfate Proteoglycans
Macrophages
Chemotaxis
Proteoglycans
Infiltration
Northern Blotting
Wound Healing
Reperfusion
Extracellular Matrix
Ligation
Rats
Real-Time Polymerase Chain Reaction
Blood Cells

Keywords

  • Coronary artery disease
  • Cytokine
  • Extracellular matrix
  • GM-CSF
  • Monocyte

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Toeda, K., Nakamura, K., Hirohata, S., Hatipoglu, O. F., Demircan, K., Yamawaki, H., ... Ninomiya, Y. (2005). Versican is induced in infiltrating monocytes in myocardial infarction. Molecular and Cellular Biochemistry, 280(1-2), 47-56. https://doi.org/10.1007/s11010-005-8051-4

Versican is induced in infiltrating monocytes in myocardial infarction. / Toeda, Kenichi; Nakamura, Keigo; Hirohata, Satoshi; Hatipoglu, Omer F.; Demircan, Kadir; Yamawaki, Hitoshi; Ogawa, Hiroko; Kusachi, Shozo; Shiratori, Yasushi; Ninomiya, Yoshifumi.

In: Molecular and Cellular Biochemistry, Vol. 280, No. 1-2, 12.2005, p. 47-56.

Research output: Contribution to journalArticle

Toeda, K, Nakamura, K, Hirohata, S, Hatipoglu, OF, Demircan, K, Yamawaki, H, Ogawa, H, Kusachi, S, Shiratori, Y & Ninomiya, Y 2005, 'Versican is induced in infiltrating monocytes in myocardial infarction', Molecular and Cellular Biochemistry, vol. 280, no. 1-2, pp. 47-56. https://doi.org/10.1007/s11010-005-8051-4
Toeda, Kenichi ; Nakamura, Keigo ; Hirohata, Satoshi ; Hatipoglu, Omer F. ; Demircan, Kadir ; Yamawaki, Hitoshi ; Ogawa, Hiroko ; Kusachi, Shozo ; Shiratori, Yasushi ; Ninomiya, Yoshifumi. / Versican is induced in infiltrating monocytes in myocardial infarction. In: Molecular and Cellular Biochemistry. 2005 ; Vol. 280, No. 1-2. pp. 47-56.
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