Verification of responses of Japanese medaka (Oryzias latipes) to anti-androgens, vinclozolin and flutamide, in short-term assays

Ataru Nakamura, Hitomi Takanobu, Ikumi Tamura, Masumi Yamamuro, Taisen Iguchi, Norihisa Tatarazako

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Various testing methods for the detection of the endocrine disruptive activities of chemicals have been developed in freshwater fish species. However, a few relatively easier specific methods for detecting anti-androgenic activities are available for fish. The aim of this study was to verify the papillary process in Japanese medaka (Oryzias latipes) as an indicator of the anti-androgenic activity of chemicals. Japanese medaka were exposed to two types of anti-androgenic compounds, vinclozolin and flutamide, using two short-term assays; one was conformed to the existing short-term reproduction assay using adult fish (adult test) and the other was a test based on the same methods but using juvenile fish at the beginning of exposure (juvenile test). Significant decreases in male papillary processes were observed in the juvenile test treated with the highest concentration of both antiandrogens (640μgl-1 vinclozolin and 1000μgl-1 flutamide); however, no significant effects were observed in the adult test. Consequently, our results indicate that papillary processes in Japanese medaka can be used as the end-point for screening the anti-androgenic activity of chemicals using juvenile fish for a specific period based on the existing short-term reproduction assay.

Original languageEnglish
Pages (from-to)545-553
Number of pages9
JournalJournal of Applied Toxicology
Volume34
Issue number5
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

vinclozolin
Oryzias
Flutamide
Fish
Androgens
Assays
Fishes
Reproduction
Androgen Antagonists
Fresh Water
Screening

Keywords

  • Anti-androgen
  • Endocrine disruption
  • Flutamide
  • Japanese medaka
  • Papillary processes
  • Vinclozolin

ASJC Scopus subject areas

  • Toxicology

Cite this

Verification of responses of Japanese medaka (Oryzias latipes) to anti-androgens, vinclozolin and flutamide, in short-term assays. / Nakamura, Ataru; Takanobu, Hitomi; Tamura, Ikumi; Yamamuro, Masumi; Iguchi, Taisen; Tatarazako, Norihisa.

In: Journal of Applied Toxicology, Vol. 34, No. 5, 2014, p. 545-553.

Research output: Contribution to journalArticle

Nakamura, Ataru ; Takanobu, Hitomi ; Tamura, Ikumi ; Yamamuro, Masumi ; Iguchi, Taisen ; Tatarazako, Norihisa. / Verification of responses of Japanese medaka (Oryzias latipes) to anti-androgens, vinclozolin and flutamide, in short-term assays. In: Journal of Applied Toxicology. 2014 ; Vol. 34, No. 5. pp. 545-553.
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