VCIP135 acts as a deubiquitinating enzyme during p97-p47-mediated reassembly of mitotic Golgi fragments

Yangzhuang Wang, Ayano Satoh, Graham Warren, Hemmo H. Meyer

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The AAA-ATPase p97/Cdc48 functions in different cellular pathways using distinct sets of adapters and other cofactors. Together with its adaptor Ufd1-Npl4, it extracts ubiquitylated substrates from the membrane for subsequent delivery to the proteasome during ER-associated degradation. Together with its adaptor p47, on the other hand, it regulates several membrane fusion events, including reassembly of Golgi cisternae after mitosis. The finding of a ubiquitin-binding domain in p47 raises the question as to whether the ubiquitin-proteasome system is also involved in membrane fusion events. Here, we show that p97-p47-mediated reassembly of Golgi cisternae requires ubiquitin, but is not dependent on proteasome-mediated proteolysis. Instead, it requires the deubiquitinating activity of one of its cofactors, VCIP135, which reverses a ubiquitylation event that occurs during mitotic disassembly. Together, these data reveal a cycle of ubiquitylation and deubiquitination that regulates Golgi membrane dynamics during mitosis. Furthermore, they represent the first evidence for a proteasome-independent function of p97/Cdc48.

Original languageEnglish
Pages (from-to)973-978
Number of pages6
JournalJournal of Cell Biology
Volume164
Issue number7
DOIs
Publication statusPublished - Mar 29 2004
Externally publishedYes

Fingerprint

Proteasome Endopeptidase Complex
Ubiquitin
Membrane Fusion
Ubiquitination
Mitosis
Membranes
Proteolysis
Deubiquitinating Enzymes

Keywords

  • Cdc48
  • Deubiquitinating enzyme
  • Membrane fusion
  • Mitosis
  • Ubiquitin

ASJC Scopus subject areas

  • Cell Biology

Cite this

VCIP135 acts as a deubiquitinating enzyme during p97-p47-mediated reassembly of mitotic Golgi fragments. / Wang, Yangzhuang; Satoh, Ayano; Warren, Graham; Meyer, Hemmo H.

In: Journal of Cell Biology, Vol. 164, No. 7, 29.03.2004, p. 973-978.

Research output: Contribution to journalArticle

Wang, Yangzhuang ; Satoh, Ayano ; Warren, Graham ; Meyer, Hemmo H. / VCIP135 acts as a deubiquitinating enzyme during p97-p47-mediated reassembly of mitotic Golgi fragments. In: Journal of Cell Biology. 2004 ; Vol. 164, No. 7. pp. 973-978.
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