Abstract
Three experiments were conducted to determine the effect of Vascular Endothelial Growth Factor (VEGF) on bovine embryonic development in vitro. Human recombinant VEGF 165 was employed at 5 ng/m/ in modified synthetic oviduct fluid. In Exp. 1, bovine cumulus oocyte complexes were matured with or without VEGF for 22 hr, inseminated without VEGF for 6 hr, then cultured with or without VEGF for 42 hr. The cleavage rate and the development rate to 4- to 8-cell were higher (P0.05) in both groups. In Exp. 3, cumulus cells were removed from presumptive embryos produced by IVM and IVF without VEGF at 10 hr Pi. Denuded embryos were cultured with or without VEGF for 38 hr or 182 hr. The cleavage rate and the development rates to 4- to 8-cell at 48 hr Pi and to blastocyst on D6, D7 and D8 were similar (P>0.05) in all groups. These results suggest that VEGF has a beneficial effect on the initial development of bovine embryo through surrounding cumulus cells.
| Original language | English |
|---|---|
| Pages (from-to) | 967-971 |
| Number of pages | 5 |
| Journal | Journal of Veterinary Medical Science |
| Volume | 64 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 2002 |
| Externally published | Yes |
Fingerprint
Keywords
- Bovine embryo
- Cumulus cell
- In vitro development
- VEGF
ASJC Scopus subject areas
- Marketing
- veterinary(all)
Cite this
Vascular endothelial growth factor (VEGF) promotes the early development of bovine embryo in the presence of cumulus cells. / Luo, Hailing; Kimura, Koji; Aoki, Mari; Hirako, Makoto.
In: Journal of Veterinary Medical Science, Vol. 64, No. 11, 11.2002, p. 967-971.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vascular endothelial growth factor (VEGF) promotes the early development of bovine embryo in the presence of cumulus cells
AU - Luo, Hailing
AU - Kimura, Koji
AU - Aoki, Mari
AU - Hirako, Makoto
PY - 2002/11
Y1 - 2002/11
N2 - Three experiments were conducted to determine the effect of Vascular Endothelial Growth Factor (VEGF) on bovine embryonic development in vitro. Human recombinant VEGF 165 was employed at 5 ng/m/ in modified synthetic oviduct fluid. In Exp. 1, bovine cumulus oocyte complexes were matured with or without VEGF for 22 hr, inseminated without VEGF for 6 hr, then cultured with or without VEGF for 42 hr. The cleavage rate and the development rate to 4- to 8-cell were higher (P0.05) in both groups. In Exp. 3, cumulus cells were removed from presumptive embryos produced by IVM and IVF without VEGF at 10 hr Pi. Denuded embryos were cultured with or without VEGF for 38 hr or 182 hr. The cleavage rate and the development rates to 4- to 8-cell at 48 hr Pi and to blastocyst on D6, D7 and D8 were similar (P>0.05) in all groups. These results suggest that VEGF has a beneficial effect on the initial development of bovine embryo through surrounding cumulus cells.
AB - Three experiments were conducted to determine the effect of Vascular Endothelial Growth Factor (VEGF) on bovine embryonic development in vitro. Human recombinant VEGF 165 was employed at 5 ng/m/ in modified synthetic oviduct fluid. In Exp. 1, bovine cumulus oocyte complexes were matured with or without VEGF for 22 hr, inseminated without VEGF for 6 hr, then cultured with or without VEGF for 42 hr. The cleavage rate and the development rate to 4- to 8-cell were higher (P0.05) in both groups. In Exp. 3, cumulus cells were removed from presumptive embryos produced by IVM and IVF without VEGF at 10 hr Pi. Denuded embryos were cultured with or without VEGF for 38 hr or 182 hr. The cleavage rate and the development rates to 4- to 8-cell at 48 hr Pi and to blastocyst on D6, D7 and D8 were similar (P>0.05) in all groups. These results suggest that VEGF has a beneficial effect on the initial development of bovine embryo through surrounding cumulus cells.
KW - Bovine embryo
KW - Cumulus cell
KW - In vitro development
KW - VEGF
UR - http://www.scopus.com/inward/record.url?scp=0041342426&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041342426&partnerID=8YFLogxK
U2 - 10.1292/jvms.64.967
DO - 10.1292/jvms.64.967
M3 - Article
VL - 64
SP - 967
EP - 971
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
SN - 0916-7250
IS - 11
ER -