Vascular endothelial growth factor (VEGF) promotes the early development of bovine embryo in the presence of cumulus cells

Hailing Luo, Koji Kimura, Mari Aoki, Makoto Hirako

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Three experiments were conducted to determine the effect of Vascular Endothelial Growth Factor (VEGF) on bovine embryonic development in vitro. Human recombinant VEGF 165 was employed at 5 ng/m/ in modified synthetic oviduct fluid. In Exp. 1, bovine cumulus oocyte complexes were matured with or without VEGF for 22 hr, inseminated without VEGF for 6 hr, then cultured with or without VEGF for 42 hr. The cleavage rate and the development rate to 4- to 8-cell were higher (P0.05) in both groups. In Exp. 3, cumulus cells were removed from presumptive embryos produced by IVM and IVF without VEGF at 10 hr Pi. Denuded embryos were cultured with or without VEGF for 38 hr or 182 hr. The cleavage rate and the development rates to 4- to 8-cell at 48 hr Pi and to blastocyst on D6, D7 and D8 were similar (P>0.05) in all groups. These results suggest that VEGF has a beneficial effect on the initial development of bovine embryo through surrounding cumulus cells.

Original languageEnglish
Pages (from-to)967-971
Number of pages5
JournalJournal of Veterinary Medical Science
Volume64
Issue number11
DOIs
Publication statusPublished - Nov 2002
Externally publishedYes

Fingerprint

Cumulus Cells
vascular endothelial growth factors
Vascular Endothelial Growth Factor A
Embryonic Development
early development
embryo (animal)
cattle
cells
Embryonic Structures
Oviducts
Blastocyst
Bovine embryo
Growth factors
Oocytes
oviducts
blastocyst
oocytes
embryogenesis

Keywords

  • Bovine embryo
  • Cumulus cell
  • In vitro development
  • VEGF

ASJC Scopus subject areas

  • Marketing
  • veterinary(all)

Cite this

Vascular endothelial growth factor (VEGF) promotes the early development of bovine embryo in the presence of cumulus cells. / Luo, Hailing; Kimura, Koji; Aoki, Mari; Hirako, Makoto.

In: Journal of Veterinary Medical Science, Vol. 64, No. 11, 11.2002, p. 967-971.

Research output: Contribution to journalArticle

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