Vascular endothelial growth factor principally acts as the main angiogenic factor in the early stage of human osteoblastogenesis

Takayuki Furumatsu, Zheng Nan Shen, Akira Kawai, Keiichiro Nishida, Hironori Manabe, Toshitaka Oohashi, Hajime Inoue, Yoshifumi Ninomiya

Research output: Contribution to journalArticlepeer-review

80 Citations (Scopus)

Abstract

Vascular endothelial growth factor (VEGF)-mediated angiogenesis is essential for bone formation. However, the effect of VEGF on osteoblastic cells during osteoblastogenesis is still controversial. The aim of this study was to clarify the relationship between osteoblastic cells derived from human mesenchymal stem cells (MSCs) and VEGF in the early stage of osteoblastic differentiation. Continuous dexamethasone treatment with a low concentration stimulated osteoblastogenesis of MSCs and the expression of VEGF121 mRNA. The VEGF secretion from osteoblastic cells also increased along with osteoblastogenesis. Neuropilin-1, which mainly binds VEGF165, was detected at all stages during early osteoblastogenesis, but VEGF receptor-1 and -2 were not detected on RT-PCR analyses. In this study, VEGF had no direct effect on the proliferation of osteoblastic cells. However, the secreted VEGF in the conditioned medium of osteoblastic cells exhibited high angiogenic power as to endothelial cell proliferation. Our findings indicated that VEGF121 principally acts as the main angiogenic factor in the early stage of human osteoblastogenesis. The present study also demonstrated the differential expression of VEGF121 during osteoblastogenesis. The increase of VEGF in the early stage might be a useful marker of induction of bone formation due to human MSCs.

Original languageEnglish
Pages (from-to)633-639
Number of pages7
JournalJournal of biochemistry
Volume133
Issue number5
DOIs
Publication statusPublished - May 1 2003

Keywords

  • Angiogenesis
  • Mesenchymal stem cells
  • Osteoblastic cells
  • Osteoblastogenesis
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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