Vascular endothelial growth factor and platelet-derived endothelial cell growth factor expression are implicated in the angiogenesis of endometrial cancer

N. Seki, J. Kodama, A. Hongo, Y. Miyagi, M. Yoshinouchi, T. Kudo

Research output: Contribution to journalArticle

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Abstract

Although many angiogenic factors have been described, it is not well defined which factors are expressed in endometrial cancer. The object of this study was to examine mRNA levels of the two angiogenic factors, vascular endothelial growth factor (VEGF) and platelet-derived endothelial cell growth factor (PD-ECGF) in endometrial cancer tissues and their association with clinicopathological features including microvessel density. The level of VEGF and PD-ECGF mRNAs was assessed by semi-quantitative reverse transcription-polymerase chain reaction using β-actin as an internal standard in 38 patients with endometrial cancer. Microvessel counts were also assessed by immunostaining for factor VIII-related antigen in the most vascularised area of the specimen. VEGF/β-actin ratios of non-endometrioid tumours were significantly higher than those of endometrioid tumours (P=0.013). VEGF/β-actin ratios of cases with lymph-vascular space involvement were significantly higher than those of cases without lymph-vascular space involvement (P=0.021). Although it was not statistically significant, PD-ECGF/β-actin ratios in grade 3 tumours were higher than those in grade 1 and 2 tumours (P=0.066). The microvessel density was significantly correlated with the level of VEGF and PD-ECGF mRNA expression (P=0.041 and P

Original languageEnglish
Pages (from-to)68-73
Number of pages6
JournalEuropean Journal of Cancer
Volume36
Issue number1
DOIs
Publication statusPublished - Jan 2000

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Thymidine Phosphorylase
Endometrial Neoplasms
Vascular Endothelial Growth Factor A
Actins
Microvessels
Angiogenesis Inducing Agents
Lymph
Messenger RNA
Blood Vessels
Neoplasms
von Willebrand Factor
Reverse Transcription
Polymerase Chain Reaction

Keywords

  • Angiogenesis
  • Endometrial cancer
  • Platelet-derived endothelial cell growth factor
  • RT-PCR
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Vascular endothelial growth factor and platelet-derived endothelial cell growth factor expression are implicated in the angiogenesis of endometrial cancer. / Seki, N.; Kodama, J.; Hongo, A.; Miyagi, Y.; Yoshinouchi, M.; Kudo, T.

In: European Journal of Cancer, Vol. 36, No. 1, 01.2000, p. 68-73.

Research output: Contribution to journalArticle

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