Varietal variation in microsatellite and caps for ethylene-related genes and its possible association with agronomic characters in melon (cucumis melo)

Y. Akashi, H. Ezura, Yasutaka Kubo, M. Masuda, Kenji Kato

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Shelf-life of fruit is one of the most important traits in melon breeding. Shelf-life of climacteric fruits is closely related with ethylene whose biosynthesis is controlled by ACC synthase and ACC oxidase genes. As the first step to develop selection markers for shelf-life in melon (Cucumis melo), microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms were analyzed in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), resulting in the establishment of six microsatellite and two CAPS markers. Five markers were developed in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeat of (TA) was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of (T)n and (A)n was also detected in CMe-ACS2. CAPS and microsatellite markers specific to vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.

Original languageEnglish
Title of host publicationActa Horticulturae
Pages313-316
Number of pages4
Volume588
Publication statusPublished - 2002

Publication series

NameActa Horticulturae
Volume588
ISSN (Print)05677572

Fingerprint

Cucumis melo
melons
agronomic traits
ethylene
microsatellite repeats
1-aminocyclopropane-1-carboxylate synthase
aminocyclopropanecarboxylate oxidase
shelf life
fruits
genes
East Asia
ethylene production
ripening
genetic polymorphism
phylogeny
breeding
cultivars

Keywords

  • Phylogeny

ASJC Scopus subject areas

  • Horticulture

Cite this

Akashi, Y., Ezura, H., Kubo, Y., Masuda, M., & Kato, K. (2002). Varietal variation in microsatellite and caps for ethylene-related genes and its possible association with agronomic characters in melon (cucumis melo). In Acta Horticulturae (Vol. 588, pp. 313-316). (Acta Horticulturae; Vol. 588).

Varietal variation in microsatellite and caps for ethylene-related genes and its possible association with agronomic characters in melon (cucumis melo). / Akashi, Y.; Ezura, H.; Kubo, Yasutaka; Masuda, M.; Kato, Kenji.

Acta Horticulturae. Vol. 588 2002. p. 313-316 (Acta Horticulturae; Vol. 588).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Akashi, Y, Ezura, H, Kubo, Y, Masuda, M & Kato, K 2002, Varietal variation in microsatellite and caps for ethylene-related genes and its possible association with agronomic characters in melon (cucumis melo). in Acta Horticulturae. vol. 588, Acta Horticulturae, vol. 588, pp. 313-316.
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