Varietal variation in microsatellite and caps for ethylene-related genes and its possible association with agronomic characters in melon (cucumis melo)

Y. Akashi, H. Ezura, Y. Kubo, M. Masuda, K. Kato

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Shelf-life of fruit is one of the most important traits in melon breeding. Shelf-life of climacteric fruits is closely related with ethylene whose biosynthesis is controlled by ACC synthase and ACC oxidase genes. As the first step to develop selection markers for shelf-life in melon (Cucumis melo), microsatellite and cleaved amplified polymorphic sequence (CAPS) polymorphisms were analyzed in two ACC synthase genes (CMe-ACS1, CMe-ACS2) and two ACC oxidase genes (CMe-ACO1, CMe-ACO3), resulting in the establishment of six microsatellite and two CAPS markers. Five markers were developed in two genes, CMe-ACS1 and CMe-ACO1, which were expressed during fruit ripening. A highly polymorphic marker was found in CMe-ACS2, where the number of simple sequence repeat of (TA) was highly variable among cultivars, ranging from seven to thirty-seven. Insertion/deletion of (T)n and (A)n was also detected in CMe-ACS2. CAPS and microsatellite markers specific to vars. makuwa and conomon, local varieties in East Asia, were detected in four markers, Acs1-c1, Acs2-ms1, Aco1-ms2 and Aco3-ms1, and could be useful markers for phylogenetic analysis in melon.

Original languageEnglish
Title of host publicationII International Symposium on Cucurbits
PublisherInternational Society for Horticultural Science
Pages313-316
Number of pages4
ISBN (Print)9789066057760
DOIs
Publication statusPublished - Jan 1 2002

Publication series

NameActa Horticulturae
Volume588
ISSN (Print)0567-7572

Keywords

  • Phylogeny

ASJC Scopus subject areas

  • Horticulture

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    Akashi, Y., Ezura, H., Kubo, Y., Masuda, M., & Kato, K. (2002). Varietal variation in microsatellite and caps for ethylene-related genes and its possible association with agronomic characters in melon (cucumis melo). In II International Symposium on Cucurbits (pp. 313-316). (Acta Horticulturae; Vol. 588). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2002.588.50