Abstract
Mutants lacking a thylakoid membrane-bound metalloprotease, FtsH, are known to cause leaf variegation in Arabidopsis. However, the effect of reduced FtsH levels on leaf variegation has scarcely been examined in other plants. In this study, we performed RNA interference (RNAi) by which FtsH expression was suppressed in tobacco. The resulting FtsH knock-down tobacco plants showed variegation in their leaves, and a negative correlation between the degree of variegation and the level of FtsH, which supported earlier observations in Arabidopsis. A decrease of NtFtsH2 as well as NtFtsH1 suggested that these are the two major isoforms comprising the FtsH complex in tobacco chloroplasts. The RNAi tobacco lines also showed photoinhibition-vulnerable phenotypes, as evidenced by high-light-sensitive PSII activity and retarded degradation of D1 protein. Interestingly, the formation of variegated sectors during leaf development appeared to differ between Arabidopsis and tobacco. In contrast to the formation of variegation in Arabidopsis, the yellow sectors in FtsH RNAi tobacco emerged from green leaves at a late stage of leaf development. A series of cytological observations implied that thylakoid membranes were dismantled after development had already occurred. Late formation of variegation in FtsH RNAi tobacco suggested that the heteromeric FtsH complex is important for maintaining thylakoid membranes.
| Original language | English |
|---|---|
| Pages (from-to) | 391-404 |
| Number of pages | 14 |
| Journal | Plant and Cell Physiology |
| Volume | 53 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2012 |
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Keywords
- Chloroplast development
- FtsH protease
- Leaf variegation
- Nicotiana tabacum (tobacco)
- Thylakoid membrane
- var2
ASJC Scopus subject areas
- Plant Science
- Physiology
- Cell Biology
Cite this
Variegated tobacco leaves generated by chloroplast FtsH suppression : Implication of ftsh function in the maintenance of thylakoid membranes. / Kato, Yusuke; Kouso, Takayoshi; Sakamoto, Wataru.
In: Plant and Cell Physiology, Vol. 53, No. 2, 02.2012, p. 391-404.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Variegated tobacco leaves generated by chloroplast FtsH suppression
T2 - Implication of ftsh function in the maintenance of thylakoid membranes
AU - Kato, Yusuke
AU - Kouso, Takayoshi
AU - Sakamoto, Wataru
PY - 2012/2
Y1 - 2012/2
N2 - Mutants lacking a thylakoid membrane-bound metalloprotease, FtsH, are known to cause leaf variegation in Arabidopsis. However, the effect of reduced FtsH levels on leaf variegation has scarcely been examined in other plants. In this study, we performed RNA interference (RNAi) by which FtsH expression was suppressed in tobacco. The resulting FtsH knock-down tobacco plants showed variegation in their leaves, and a negative correlation between the degree of variegation and the level of FtsH, which supported earlier observations in Arabidopsis. A decrease of NtFtsH2 as well as NtFtsH1 suggested that these are the two major isoforms comprising the FtsH complex in tobacco chloroplasts. The RNAi tobacco lines also showed photoinhibition-vulnerable phenotypes, as evidenced by high-light-sensitive PSII activity and retarded degradation of D1 protein. Interestingly, the formation of variegated sectors during leaf development appeared to differ between Arabidopsis and tobacco. In contrast to the formation of variegation in Arabidopsis, the yellow sectors in FtsH RNAi tobacco emerged from green leaves at a late stage of leaf development. A series of cytological observations implied that thylakoid membranes were dismantled after development had already occurred. Late formation of variegation in FtsH RNAi tobacco suggested that the heteromeric FtsH complex is important for maintaining thylakoid membranes.
AB - Mutants lacking a thylakoid membrane-bound metalloprotease, FtsH, are known to cause leaf variegation in Arabidopsis. However, the effect of reduced FtsH levels on leaf variegation has scarcely been examined in other plants. In this study, we performed RNA interference (RNAi) by which FtsH expression was suppressed in tobacco. The resulting FtsH knock-down tobacco plants showed variegation in their leaves, and a negative correlation between the degree of variegation and the level of FtsH, which supported earlier observations in Arabidopsis. A decrease of NtFtsH2 as well as NtFtsH1 suggested that these are the two major isoforms comprising the FtsH complex in tobacco chloroplasts. The RNAi tobacco lines also showed photoinhibition-vulnerable phenotypes, as evidenced by high-light-sensitive PSII activity and retarded degradation of D1 protein. Interestingly, the formation of variegated sectors during leaf development appeared to differ between Arabidopsis and tobacco. In contrast to the formation of variegation in Arabidopsis, the yellow sectors in FtsH RNAi tobacco emerged from green leaves at a late stage of leaf development. A series of cytological observations implied that thylakoid membranes were dismantled after development had already occurred. Late formation of variegation in FtsH RNAi tobacco suggested that the heteromeric FtsH complex is important for maintaining thylakoid membranes.
KW - Chloroplast development
KW - FtsH protease
KW - Leaf variegation
KW - Nicotiana tabacum (tobacco)
KW - Thylakoid membrane
KW - var2
UR - http://www.scopus.com/inward/record.url?scp=84863412992&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863412992&partnerID=8YFLogxK
U2 - 10.1093/pcp/pcr189
DO - 10.1093/pcp/pcr189
M3 - Article
C2 - 22197884
AN - SCOPUS:84863412992
VL - 53
SP - 391
EP - 404
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 2
ER -