Variations in Cellular Polyamine Compositions and Contents of Vibrio Species during Growth in Media with Various NaCl Concentrations

Shigeo Yamamoto, Motoko Yoshida, Hiroshi Nakao, Miyoko Koyama, Yoshie Hashimoto, Sumio Shinoda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In order to obtain insight into the possible physiological significance of norspermidine [NH2(CH2)3NH(CH2)3NH2], which is characteristically present in the genus Vibrio, we have determined the cellular polyamine contents of Vibrio alginolyticus and V. parahaemolyticus during growth in media with various NaCl concentrations. Regardless of the medium NaCl concentration, the norspermidine content, on a per mg of protein basis, was highest in the early exponential phase and then declined with prolonged cultivation, whereas there was only a slight change in spermidine, the content of which was much lower than that of norspermidine throughout growth. Moreover, at higher NaCl concentrations, norspermidine was the major polyamine throughout growth. When the cells were suddenly exposed to media with various NaCl concentrations, the content of norspermidine increased immediately or after an initial precipitous drop with the onset of growth, but that of spermidine did not change markedly. The putrescine content of these vibrios was substantially elevated only when the cells were grown in or transferred to media with lower NaCl concentration. This pattern of polyamines suggests that, instead of spermidine, norspermidine might play some important role(s) in supporting growth, whereas putrescine may be mainly involved in maintaining cellular ionic or osmotic balance against a lower NaCl environment.

Original languageEnglish
Pages (from-to)3038-3042
Number of pages5
JournalChemical and Pharmaceutical Bulletin
Volume34
Issue number7
DOIs
Publication statusPublished - 1986

Fingerprint

Vibrio
Polyamines
Spermidine
Growth
Chemical analysis
Putrescine
Vibrio alginolyticus
norspermidine
Proteins

Keywords

  • medium sodium chloride concentration
  • norspermidine
  • polyamine content variation
  • putrescine
  • Vibrio
  • Vibrio alginolyticus
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

Cite this

Variations in Cellular Polyamine Compositions and Contents of Vibrio Species during Growth in Media with Various NaCl Concentrations. / Yamamoto, Shigeo; Yoshida, Motoko; Nakao, Hiroshi; Koyama, Miyoko; Hashimoto, Yoshie; Shinoda, Sumio.

In: Chemical and Pharmaceutical Bulletin, Vol. 34, No. 7, 1986, p. 3038-3042.

Research output: Contribution to journalArticle

Yamamoto, Shigeo ; Yoshida, Motoko ; Nakao, Hiroshi ; Koyama, Miyoko ; Hashimoto, Yoshie ; Shinoda, Sumio. / Variations in Cellular Polyamine Compositions and Contents of Vibrio Species during Growth in Media with Various NaCl Concentrations. In: Chemical and Pharmaceutical Bulletin. 1986 ; Vol. 34, No. 7. pp. 3038-3042.
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