Abstract
In order to obtain insight into the possible physiological significance of norspermidine [NH2(CH2)3NH(CH2)3NH2], which is characteristically present in the genus Vibrio, we have determined the cellular polyamine contents of Vibrio alginolyticus and V. parahaemolyticus during growth in media with various NaCl concentrations. Regardless of the medium NaCl concentration, the norspermidine content, on a per mg of protein basis, was highest in the early exponential phase and then declined with prolonged cultivation, whereas there was only a slight change in spermidine, the content of which was much lower than that of norspermidine throughout growth. Moreover, at higher NaCl concentrations, norspermidine was the major polyamine throughout growth. When the cells were suddenly exposed to media with various NaCl concentrations, the content of norspermidine increased immediately or after an initial precipitous drop with the onset of growth, but that of spermidine did not change markedly. The putrescine content of these vibrios was substantially elevated only when the cells were grown in or transferred to media with lower NaCl concentration. This pattern of polyamines suggests that, instead of spermidine, norspermidine might play some important role(s) in supporting growth, whereas putrescine may be mainly involved in maintaining cellular ionic or osmotic balance against a lower NaCl environment.
| Original language | English |
|---|---|
| Pages (from-to) | 3038-3042 |
| Number of pages | 5 |
| Journal | Chemical and Pharmaceutical Bulletin |
| Volume | 34 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1986 |
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Keywords
- medium sodium chloride concentration
- norspermidine
- polyamine content variation
- putrescine
- Vibrio
- Vibrio alginolyticus
- Vibrio parahaemolyticus
ASJC Scopus subject areas
- Chemistry(all)
- Drug Discovery
Cite this
Variations in Cellular Polyamine Compositions and Contents of Vibrio Species during Growth in Media with Various NaCl Concentrations. / Yamamoto, Shigeo; Yoshida, Motoko; Nakao, Hiroshi; Koyama, Miyoko; Hashimoto, Yoshie; Shinoda, Sumio.
In: Chemical and Pharmaceutical Bulletin, Vol. 34, No. 7, 1986, p. 3038-3042.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Variations in Cellular Polyamine Compositions and Contents of Vibrio Species during Growth in Media with Various NaCl Concentrations
AU - Yamamoto, Shigeo
AU - Yoshida, Motoko
AU - Nakao, Hiroshi
AU - Koyama, Miyoko
AU - Hashimoto, Yoshie
AU - Shinoda, Sumio
PY - 1986
Y1 - 1986
N2 - In order to obtain insight into the possible physiological significance of norspermidine [NH2(CH2)3NH(CH2)3NH2], which is characteristically present in the genus Vibrio, we have determined the cellular polyamine contents of Vibrio alginolyticus and V. parahaemolyticus during growth in media with various NaCl concentrations. Regardless of the medium NaCl concentration, the norspermidine content, on a per mg of protein basis, was highest in the early exponential phase and then declined with prolonged cultivation, whereas there was only a slight change in spermidine, the content of which was much lower than that of norspermidine throughout growth. Moreover, at higher NaCl concentrations, norspermidine was the major polyamine throughout growth. When the cells were suddenly exposed to media with various NaCl concentrations, the content of norspermidine increased immediately or after an initial precipitous drop with the onset of growth, but that of spermidine did not change markedly. The putrescine content of these vibrios was substantially elevated only when the cells were grown in or transferred to media with lower NaCl concentration. This pattern of polyamines suggests that, instead of spermidine, norspermidine might play some important role(s) in supporting growth, whereas putrescine may be mainly involved in maintaining cellular ionic or osmotic balance against a lower NaCl environment.
AB - In order to obtain insight into the possible physiological significance of norspermidine [NH2(CH2)3NH(CH2)3NH2], which is characteristically present in the genus Vibrio, we have determined the cellular polyamine contents of Vibrio alginolyticus and V. parahaemolyticus during growth in media with various NaCl concentrations. Regardless of the medium NaCl concentration, the norspermidine content, on a per mg of protein basis, was highest in the early exponential phase and then declined with prolonged cultivation, whereas there was only a slight change in spermidine, the content of which was much lower than that of norspermidine throughout growth. Moreover, at higher NaCl concentrations, norspermidine was the major polyamine throughout growth. When the cells were suddenly exposed to media with various NaCl concentrations, the content of norspermidine increased immediately or after an initial precipitous drop with the onset of growth, but that of spermidine did not change markedly. The putrescine content of these vibrios was substantially elevated only when the cells were grown in or transferred to media with lower NaCl concentration. This pattern of polyamines suggests that, instead of spermidine, norspermidine might play some important role(s) in supporting growth, whereas putrescine may be mainly involved in maintaining cellular ionic or osmotic balance against a lower NaCl environment.
KW - medium sodium chloride concentration
KW - norspermidine
KW - polyamine content variation
KW - putrescine
KW - Vibrio
KW - Vibrio alginolyticus
KW - Vibrio parahaemolyticus
UR - http://www.scopus.com/inward/record.url?scp=0022747842&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022747842&partnerID=8YFLogxK
U2 - 10.1248/cpb.34.3038
DO - 10.1248/cpb.34.3038
M3 - Article
VL - 34
SP - 3038
EP - 3042
JO - Chemical and Pharmaceutical Bulletin
JF - Chemical and Pharmaceutical Bulletin
SN - 0009-2363
IS - 7
ER -