Variations in bacterial communities in laboratory-scale and big bale silos assessed by fermentation products, colony counts and denaturing gradient gel electrophoresis profiles

Naoki Nishino, T. Yuji

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aims: To assess the variation in bacterial communities in laboratory-scale and big bale silos. Methods and Results: Wilted Italian ryegrass (628 g dry matter kg-1) was ensiled in vacuum-packed plastic pouches and big bales. Silos were opened after 3 months, and the fermentation products, colony counts and denaturing gradient gel electrophoresis (DGGE) profiles were determined. Eight samples were collected separately from a big bale, while one representative sample was taken from a plastic pouch. Significant variation was found between big bales in dry matter, ethanol, lactic acid, acetic acid and ammonia-N contents. No differences were shown between plastic pouches and big bales, except that more ethanol was produced in the former air-tight silos. Plastic pouches could resemble a specific silo and outer sampling sites of big bales based on fermentation products and DGGE profiles respectively. Conclusions: Considerable variation in fermentation products may exist between big bale silos. Plastic pouches can serve as a model of big bale silos, although they do not provide information on the heterogeneity within and between bales. Significance and Impact of the Study: Assessment of bacterial communities associated with ensiling can differ according to the criteria of fermentation products, colony counts and DGGE profiles.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalLetters in Applied Microbiology
Volume46
Issue number3
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Denaturing Gradient Gel Electrophoresis
Electrophoresis
Fermentation
Plastics
fermentation
electrokinesis
Gels
gel
plastic
Ethanol
dry matter
ethanol
Lolium
Lactic acid
Vacuum
Ammonia
Acetic acid
Acetic Acid
Lactic Acid
acetic acid

Keywords

  • Big bale
  • Cluster analysis
  • Denaturing gradient gel electrophoresis
  • Silage

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Water Science and Technology
  • Fluid Flow and Transfer Processes
  • Microbiology
  • Ecology

Cite this

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abstract = "Aims: To assess the variation in bacterial communities in laboratory-scale and big bale silos. Methods and Results: Wilted Italian ryegrass (628 g dry matter kg-1) was ensiled in vacuum-packed plastic pouches and big bales. Silos were opened after 3 months, and the fermentation products, colony counts and denaturing gradient gel electrophoresis (DGGE) profiles were determined. Eight samples were collected separately from a big bale, while one representative sample was taken from a plastic pouch. Significant variation was found between big bales in dry matter, ethanol, lactic acid, acetic acid and ammonia-N contents. No differences were shown between plastic pouches and big bales, except that more ethanol was produced in the former air-tight silos. Plastic pouches could resemble a specific silo and outer sampling sites of big bales based on fermentation products and DGGE profiles respectively. Conclusions: Considerable variation in fermentation products may exist between big bale silos. Plastic pouches can serve as a model of big bale silos, although they do not provide information on the heterogeneity within and between bales. Significance and Impact of the Study: Assessment of bacterial communities associated with ensiling can differ according to the criteria of fermentation products, colony counts and DGGE profiles.",
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AB - Aims: To assess the variation in bacterial communities in laboratory-scale and big bale silos. Methods and Results: Wilted Italian ryegrass (628 g dry matter kg-1) was ensiled in vacuum-packed plastic pouches and big bales. Silos were opened after 3 months, and the fermentation products, colony counts and denaturing gradient gel electrophoresis (DGGE) profiles were determined. Eight samples were collected separately from a big bale, while one representative sample was taken from a plastic pouch. Significant variation was found between big bales in dry matter, ethanol, lactic acid, acetic acid and ammonia-N contents. No differences were shown between plastic pouches and big bales, except that more ethanol was produced in the former air-tight silos. Plastic pouches could resemble a specific silo and outer sampling sites of big bales based on fermentation products and DGGE profiles respectively. Conclusions: Considerable variation in fermentation products may exist between big bale silos. Plastic pouches can serve as a model of big bale silos, although they do not provide information on the heterogeneity within and between bales. Significance and Impact of the Study: Assessment of bacterial communities associated with ensiling can differ according to the criteria of fermentation products, colony counts and DGGE profiles.

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