Variation of cell spreading on TiO2 film modified by 775 nm and 388 nm femtosecond laser irradiation

M. Tsukamoto, T. Shinonaga, Y. Sato, P. Chen, A. Nagai, T. Hanawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)


Titanium (Ti) is one of the most used biomaterials in metals. However, Ti is typically artificial materials. Thus, it is necessary for improving the biocompatibility of Ti. Recently, coating of the titanium dioxides (TiO 2) film on Ti plate has been proposed to improve biocompatibility of Ti. We have developed coating method of the film on Ti plate with an aerosol beam. Periodic structures formation on biomaterials was also a useful method for improving the biocompatibility. Direction of cell spreading might be controlled along the grooves of periodic microstructures. In our previous study, periodic nanostructures were formed on the film by femtosecond laser irradiation at fundamental wave (775 nm). Period of the periodic nanostructures was about 230 nm. In cell test, cell spreading along the grooves of the periodic nanostructures was observed although it was not done for the film without the periodic nanostructures. Then, influence of the period of the periodic nanostructures on cell spreading has not been investigated yet. The period might be changed by changing the laser wavelength. In this study, the periodic nanostructures were created on the film with femtosecond laser at 775nm and 388 nm, respectively. After cell test, cell spreading along the grooves of the periodic nanostructures was observed on 775 nm and 388nm laser irradiated areas. Distribution of direction of cell spreading on laser irradiated area was also examined. These results suggested that controlling the cell spreading on periodic nanostructures with period of 230 nm was better than that with period of 130 nm.

Original languageEnglish
Title of host publicationSynthesis and Photonics of Nanoscale Materials XI
ISBN (Print)9780819498823
Publication statusPublished - Jan 1 2014
Externally publishedYes
EventSynthesis and Photonics of Nanoscale Materials XI - San Francisco, CA, United States
Duration: Feb 2 2014Feb 4 2014

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


OtherSynthesis and Photonics of Nanoscale Materials XI
Country/TerritoryUnited States
CitySan Francisco, CA


  • Aerosol beam
  • Biocompatibility
  • Cell spreading
  • Femtosecond laser
  • Periodic nanostructures

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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