Variable expression on lung cancer cell lines of HLA-A2-binding MAGE-3 peptide recognized by cytotoxic T lymphocytes

Toshiyuki Takaki, Akio Hiraki, Akiko Uenaka, Shinya Gomi, Kyogo Itoh, Heiichiro Udono, Akira Shibuya, Takao Tsuji, Sadayoshi Sekiguchi, Eiichi Nakayama

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16 Citations (Scopus)

Abstract

Cytotoxic T lymphocytes (CTL) specific for HLA-A2-binding MAGE-3 peptide (FLWGPRALV) were generated by repetitive stimulation of PBMC with the peptide in the presence of EBV-transformed B blasts and IL-2. Using these CTL, we investigated the expression of the HLA-A2-binding MAGE-3 peptide on lung cancer cell lines. Of 14 cell lines investigated, 1-87, PC-9, OU-LC-KI, 11- 18 and LK87 were derived from HLA-A2 positive patients. But cytofluorometry analysis showed that 1-87, PC-9 and OU-LC-KI, but not 11-18 or LK87 expressed the HLA-A2 antigen. All five cell lines expressed MAGE-3 gene mRNA. Twelve of thirteen CTL lines from two HLA-A2 positive donors showed no cytotoxicity against any of the 14 lung cancer cell lines. CTL line TI-1 showed cytotoxicity against 1-87 but not against any of the other cell lines. Treatment of 1-87 with IFN-γ greatly augmented the cytotoxicity of TI-1 and induced it in the other 12 CTL lines, confirming the expression of the peptide on 1-87. No cytotoxicity was induced by IFN-γ treatment of PC-9 or OU-LC-KI. However, PC-9 and OU-LC-KI pulsed with the peptide were killed efficiently by all of the CTL lines, suggesting no expression of the peptide on those cells. A low level of cytotoxicity was induced on 11-18 but not LK87 by IFN-γ treatment, although expression of the HLA-A2 antigen was not observed by cytofluorometry. These findings showed that expression of the HLA-A2-binding MAGE-3 peptide recognized by CTL was variable on lung cancer cell lines.

Original languageEnglish
Pages (from-to)1103-1109
Number of pages7
JournalInternational journal of oncology
Volume12
Issue number5
DOIs
Publication statusPublished - May 1998

Keywords

  • A2-binding MAGE-3 peptide
  • Cytotoxic T lymphocytes
  • Lung cancer cell line
  • Peripheral blood mononuclear cells

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Takaki, T., Hiraki, A., Uenaka, A., Gomi, S., Itoh, K., Udono, H., Shibuya, A., Tsuji, T., Sekiguchi, S., & Nakayama, E. (1998). Variable expression on lung cancer cell lines of HLA-A2-binding MAGE-3 peptide recognized by cytotoxic T lymphocytes. International journal of oncology, 12(5), 1103-1109. https://doi.org/10.3892/ijo.12.5.1103