Variable Expression of Epstein-Barr Virus Latent Membrane Protein I in Reed-Sternberg Cells of Hodgkin's Disease

Norihiro Teramoto, Liu Cao, Nobuhiro Kawasaki, Yuji Tonoyama, Ashit Baran Sarker, Tadashi Yoshino, Kiyoshi Takahashi, Tadaatsu Akagi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Reed-Sternberg cells (RS cells) of Hodgkin's disease (HD) are frequently infected with Epstein-Barr virus (EBV) and express EBV-encoded nonpolyadenylated RNA transcripts (EBER)-1. EBV latency has been classified into three distinct forms: Latency I, expressing only one of the latent proteins, EBV nuclear antigen (EBNA)-1, latency II, coexpressing EBNA-1 and LMPs, and latency III, expressing all latent viral proteins. RS cells express LMP-1 in addition to EBNA-1 and are considered to be in EBV latency II frequently encountered in nasopharyngeal carcinoma. We examined 13 cases of EBV-infected HD by combined EBER-1 in situ hybridization and immunostaining for LMP-1. All of the RS cells expressed EBER-1, but a substantial number of EBER-1 + RS cells were negative for LMP-1. The percentage of LMP-1 + RS cells out of EBER-1 + RS cells varied from 7% to 100% (average 69%). In this study, we showed that all EBV-infected RS cells were not restricted to latency II, and some belonged to latency I.

Original languageEnglish
Pages (from-to)267-270
Number of pages4
JournalActa Medica Okayama
Volume50
Issue number5
Publication statusPublished - Oct 1996

Fingerprint

Reed-Sternberg Cells
Human Herpesvirus 4
Hodgkin Disease
Viruses
Membrane Proteins
Epstein-Barr Virus Nuclear Antigens
RNA
Virus Latency
Viral Proteins
In Situ Hybridization

Keywords

  • EBER-1
  • Immunohistochemistry
  • In situ hybridization
  • Latency

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Teramoto, N., Cao, L., Kawasaki, N., Tonoyama, Y., Sarker, A. B., Yoshino, T., ... Akagi, T. (1996). Variable Expression of Epstein-Barr Virus Latent Membrane Protein I in Reed-Sternberg Cells of Hodgkin's Disease. Acta Medica Okayama, 50(5), 267-270.

Variable Expression of Epstein-Barr Virus Latent Membrane Protein I in Reed-Sternberg Cells of Hodgkin's Disease. / Teramoto, Norihiro; Cao, Liu; Kawasaki, Nobuhiro; Tonoyama, Yuji; Sarker, Ashit Baran; Yoshino, Tadashi; Takahashi, Kiyoshi; Akagi, Tadaatsu.

In: Acta Medica Okayama, Vol. 50, No. 5, 10.1996, p. 267-270.

Research output: Contribution to journalArticle

Teramoto, N, Cao, L, Kawasaki, N, Tonoyama, Y, Sarker, AB, Yoshino, T, Takahashi, K & Akagi, T 1996, 'Variable Expression of Epstein-Barr Virus Latent Membrane Protein I in Reed-Sternberg Cells of Hodgkin's Disease', Acta Medica Okayama, vol. 50, no. 5, pp. 267-270.
Teramoto, Norihiro ; Cao, Liu ; Kawasaki, Nobuhiro ; Tonoyama, Yuji ; Sarker, Ashit Baran ; Yoshino, Tadashi ; Takahashi, Kiyoshi ; Akagi, Tadaatsu. / Variable Expression of Epstein-Barr Virus Latent Membrane Protein I in Reed-Sternberg Cells of Hodgkin's Disease. In: Acta Medica Okayama. 1996 ; Vol. 50, No. 5. pp. 267-270.
@article{3bb44ec0753f459f8d51b6d73b6ce59c,
title = "Variable Expression of Epstein-Barr Virus Latent Membrane Protein I in Reed-Sternberg Cells of Hodgkin's Disease",
abstract = "Reed-Sternberg cells (RS cells) of Hodgkin's disease (HD) are frequently infected with Epstein-Barr virus (EBV) and express EBV-encoded nonpolyadenylated RNA transcripts (EBER)-1. EBV latency has been classified into three distinct forms: Latency I, expressing only one of the latent proteins, EBV nuclear antigen (EBNA)-1, latency II, coexpressing EBNA-1 and LMPs, and latency III, expressing all latent viral proteins. RS cells express LMP-1 in addition to EBNA-1 and are considered to be in EBV latency II frequently encountered in nasopharyngeal carcinoma. We examined 13 cases of EBV-infected HD by combined EBER-1 in situ hybridization and immunostaining for LMP-1. All of the RS cells expressed EBER-1, but a substantial number of EBER-1 + RS cells were negative for LMP-1. The percentage of LMP-1 + RS cells out of EBER-1 + RS cells varied from 7{\%} to 100{\%} (average 69{\%}). In this study, we showed that all EBV-infected RS cells were not restricted to latency II, and some belonged to latency I.",
keywords = "EBER-1, Immunohistochemistry, In situ hybridization, Latency",
author = "Norihiro Teramoto and Liu Cao and Nobuhiro Kawasaki and Yuji Tonoyama and Sarker, {Ashit Baran} and Tadashi Yoshino and Kiyoshi Takahashi and Tadaatsu Akagi",
year = "1996",
month = "10",
language = "English",
volume = "50",
pages = "267--270",
journal = "Acta Medica Okayama",
issn = "0386-300X",
publisher = "Okayama University",
number = "5",

}

TY - JOUR

T1 - Variable Expression of Epstein-Barr Virus Latent Membrane Protein I in Reed-Sternberg Cells of Hodgkin's Disease

AU - Teramoto, Norihiro

AU - Cao, Liu

AU - Kawasaki, Nobuhiro

AU - Tonoyama, Yuji

AU - Sarker, Ashit Baran

AU - Yoshino, Tadashi

AU - Takahashi, Kiyoshi

AU - Akagi, Tadaatsu

PY - 1996/10

Y1 - 1996/10

N2 - Reed-Sternberg cells (RS cells) of Hodgkin's disease (HD) are frequently infected with Epstein-Barr virus (EBV) and express EBV-encoded nonpolyadenylated RNA transcripts (EBER)-1. EBV latency has been classified into three distinct forms: Latency I, expressing only one of the latent proteins, EBV nuclear antigen (EBNA)-1, latency II, coexpressing EBNA-1 and LMPs, and latency III, expressing all latent viral proteins. RS cells express LMP-1 in addition to EBNA-1 and are considered to be in EBV latency II frequently encountered in nasopharyngeal carcinoma. We examined 13 cases of EBV-infected HD by combined EBER-1 in situ hybridization and immunostaining for LMP-1. All of the RS cells expressed EBER-1, but a substantial number of EBER-1 + RS cells were negative for LMP-1. The percentage of LMP-1 + RS cells out of EBER-1 + RS cells varied from 7% to 100% (average 69%). In this study, we showed that all EBV-infected RS cells were not restricted to latency II, and some belonged to latency I.

AB - Reed-Sternberg cells (RS cells) of Hodgkin's disease (HD) are frequently infected with Epstein-Barr virus (EBV) and express EBV-encoded nonpolyadenylated RNA transcripts (EBER)-1. EBV latency has been classified into three distinct forms: Latency I, expressing only one of the latent proteins, EBV nuclear antigen (EBNA)-1, latency II, coexpressing EBNA-1 and LMPs, and latency III, expressing all latent viral proteins. RS cells express LMP-1 in addition to EBNA-1 and are considered to be in EBV latency II frequently encountered in nasopharyngeal carcinoma. We examined 13 cases of EBV-infected HD by combined EBER-1 in situ hybridization and immunostaining for LMP-1. All of the RS cells expressed EBER-1, but a substantial number of EBER-1 + RS cells were negative for LMP-1. The percentage of LMP-1 + RS cells out of EBER-1 + RS cells varied from 7% to 100% (average 69%). In this study, we showed that all EBV-infected RS cells were not restricted to latency II, and some belonged to latency I.

KW - EBER-1

KW - Immunohistochemistry

KW - In situ hybridization

KW - Latency

UR - http://www.scopus.com/inward/record.url?scp=0030254585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030254585&partnerID=8YFLogxK

M3 - Article

C2 - 8914680

AN - SCOPUS:0030254585

VL - 50

SP - 267

EP - 270

JO - Acta Medica Okayama

JF - Acta Medica Okayama

SN - 0386-300X

IS - 5

ER -