Variability, stability, and resilience of fecal microbiota in dairy cows fed whole crop corn silage

Minh Thuy Tang, Hongyan Han, Zhu Yu, Takeshi Tsuruta, Naoki Nishino

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The microbiota of whole crop corn silage and feces of silage-fed dairy cows were examined. A total of 18 dairy cow feces were collected from six farms in Japan and China, and high-throughput Illumina sequencing of the V4 hypervariable region of 16S rRNA genes was performed. Lactobacillaceae were dominant in all silages, followed by Acetobacteraceae, Bacillaceae, and Enterobacteriaceae. In feces, the predominant families were Ruminococcaceae, Bacteroidaceae, Clostridiaceae, Lachnospiraceae, Rikenellaceae, and Paraprevotellaceae. Therefore, Lactobacillaceae of corn silage appeared to be eliminated in the gastrointestinal tract. Although fecal microbiota composition was similar in most samples, relative abundances of several families, such as Ruminococcaceae, Christensenellaceae, Turicibacteraceae, and Succinivibrionaceae, varied between farms and countries. In addition to the geographical location, differences in feeding management between total mixed ration feeding and separate feeding appeared to be involved in the variations. Moreover, a cow-to-cow variation for concentrate-associated families was demonstrated at the same farm; two cows showed high abundance of Succinivibrionaceae and Prevotellaceae, whereas another had a high abundance of Porphyromonadaceae. There was a negative correlation between forage-associated Ruminococcaceae and concentrate-associated Succinivibrionaceae and Prevotellaceae in 18 feces samples. Succinivibrionaceae, Prevotellaceae, p-2534-18B5, and Spirochaetaceae were regarded as highly variable taxa in this study. These findings help to improve our understanding of variation and similarity of the fecal microbiota of dairy cows with regard to individuals, farms, and countries. Microbiota of naturally fermented corn silage had no influence on the fecal microbiota of dairy cows.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalApplied Microbiology and Biotechnology
DOIs
Publication statusAccepted/In press - Jun 10 2017

Fingerprint

Succinivibrionaceae
Silage
Microbiota
Zea mays
Feces
Lactobacillaceae
Acetobacteraceae
Spirochaetaceae
Bacillaceae
Bacteroidaceae
Enterobacteriaceae
rRNA Genes
Gastrointestinal Tract
China
Japan
Farms

Keywords

  • Dairy cows
  • Feces
  • Microbiota
  • Next-generation sequencing
  • Silage
  • Variation

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Variability, stability, and resilience of fecal microbiota in dairy cows fed whole crop corn silage. / Tang, Minh Thuy; Han, Hongyan; Yu, Zhu; Tsuruta, Takeshi; Nishino, Naoki.

In: Applied Microbiology and Biotechnology, 10.06.2017, p. 1-10.

Research output: Contribution to journalArticle

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