Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51

Kazuhiko Ochiai; Yasunaga Yoshikawa; Kumiko Yoshimatsu; Toshina Oonuma; Yukiko Tomioka; Eichi Takeda; Jiro Arikawa; Katsumi Mominoki; Toshinori Omi; Kazuyoshi Hashizume; Masami Morimatsu

doi:10.1016/j.febslet.2011.05.027

Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51

Kazuhiko Ochiai, Yasunaga Yoshikawa, Kumiko Yoshimatsu, Toshina Oonuma, Yukiko Tomioka, Eichi Takeda, Jiro Arikawa, Katsumi Mominoki, Toshinori Omi, Kazuyoshi Hashizume, Masami Morimatsu

Advanced Science Research Center

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The breast cancer susceptibility protein BRCA2 is essential for recombinational DNA repair. BRCA2 specifically binds to RAD51 via eight BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks. In this study, a mammalian two-hybrid assay and competitive ELISA showed that the interaction between BRC repeat 4 (BRC4) and RAD51 was strengthened by the substitution of a single BRC4 amino acid from valine to isoleucine (V1532I). However, the cancer-associated V1532F mutant exhibited very weak interaction with RAD51. This study used a comparative analysis of BRC4 between animal species to identify V1532 as an important residue that interacts with RAD51.

Original language	English
Pages (from-to)	1771-1777
Number of pages	7
Journal	FEBS Letters
Volume	585
Issue number	12
DOIs	https://doi.org/10.1016/j.febslet.2011.05.027
Publication status	Published - Jun 23 2011

Keywords

BRC repeat
BRCA2
Canine
Homologous recombination
RAD51

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2011.05.027

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title = "Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51",

abstract = "The breast cancer susceptibility protein BRCA2 is essential for recombinational DNA repair. BRCA2 specifically binds to RAD51 via eight BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks. In this study, a mammalian two-hybrid assay and competitive ELISA showed that the interaction between BRC repeat 4 (BRC4) and RAD51 was strengthened by the substitution of a single BRC4 amino acid from valine to isoleucine (V1532I). However, the cancer-associated V1532F mutant exhibited very weak interaction with RAD51. This study used a comparative analysis of BRC4 between animal species to identify V1532 as an important residue that interacts with RAD51.",

keywords = "BRC repeat, BRCA2, Canine, Homologous recombination, RAD51",

author = "Kazuhiko Ochiai and Yasunaga Yoshikawa and Kumiko Yoshimatsu and Toshina Oonuma and Yukiko Tomioka and Eichi Takeda and Jiro Arikawa and Katsumi Mominoki and Toshinori Omi and Kazuyoshi Hashizume and Masami Morimatsu",

note = "Funding Information: This work was supported in part by Grants-in-aid for Scientific Research 15208030 , 15380201 , 11460133 , 22791476 ) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan and supported by a Kitasato University Research Grant for Young Researchers to Dr. Y. Yoshikawa, and Grant for Encouragement of Young Scientists to Y. Yoshikawa from School of Veterinary Medicine, Kitasato University. ",

year = "2011",

month = jun,

day = "23",

doi = "10.1016/j.febslet.2011.05.027",

language = "English",

volume = "585",

pages = "1771--1777",

journal = "FEBS Letters",

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T1 - Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51

AU - Ochiai, Kazuhiko

AU - Yoshikawa, Yasunaga

AU - Yoshimatsu, Kumiko

AU - Oonuma, Toshina

AU - Tomioka, Yukiko

AU - Takeda, Eichi

AU - Arikawa, Jiro

AU - Mominoki, Katsumi

AU - Omi, Toshinori

AU - Hashizume, Kazuyoshi

AU - Morimatsu, Masami

N1 - Funding Information: This work was supported in part by Grants-in-aid for Scientific Research 15208030 , 15380201 , 11460133 , 22791476 ) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan and supported by a Kitasato University Research Grant for Young Researchers to Dr. Y. Yoshikawa, and Grant for Encouragement of Young Scientists to Y. Yoshikawa from School of Veterinary Medicine, Kitasato University.

PY - 2011/6/23

Y1 - 2011/6/23

N2 - The breast cancer susceptibility protein BRCA2 is essential for recombinational DNA repair. BRCA2 specifically binds to RAD51 via eight BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks. In this study, a mammalian two-hybrid assay and competitive ELISA showed that the interaction between BRC repeat 4 (BRC4) and RAD51 was strengthened by the substitution of a single BRC4 amino acid from valine to isoleucine (V1532I). However, the cancer-associated V1532F mutant exhibited very weak interaction with RAD51. This study used a comparative analysis of BRC4 between animal species to identify V1532 as an important residue that interacts with RAD51.

AB - The breast cancer susceptibility protein BRCA2 is essential for recombinational DNA repair. BRCA2 specifically binds to RAD51 via eight BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks. In this study, a mammalian two-hybrid assay and competitive ELISA showed that the interaction between BRC repeat 4 (BRC4) and RAD51 was strengthened by the substitution of a single BRC4 amino acid from valine to isoleucine (V1532I). However, the cancer-associated V1532F mutant exhibited very weak interaction with RAD51. This study used a comparative analysis of BRC4 between animal species to identify V1532 as an important residue that interacts with RAD51.

KW - BRC repeat

KW - BRCA2

KW - Canine

KW - Homologous recombination

KW - RAD51

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Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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