Validation of transrectal ultrasonographic volumetry for orthotopic prostate tumours in mice

R. Kraaij, W. M. Van Weerden, C. M A De Ridder, E. J. Gussenhoven, J. Honkoop, Yasutomo Nasu, C. H. Bangma

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Orthotopic human prostate tumour models in athymic nude mice are regarded as being most suitable for fundamental and pre-clinical research on prostate cancer. The anatomic localization of the tumour in the pelvis, however, provides little possibility for monitoring tumour growth or regression. To assess time-related changes in orthotopic tumour volume, we applied transrectal ultrasonography (TRUS) to the murine prostate. This technique has the advantages of allowing accurate monitoring of tumours during therapeutic manipulations and a reduction of animal use due to a reduction of sacrificing endpoints. To validate the TRUS method, the mouse prostate reconstitution model, RM-9, and the prostate-specific antigen (PSA) producing human prostate cancer xenograft PC-346 were used. Volumetric calliper measurements were performed with a 30 MHz ultrasound probe designed for intra-arterial use in humans. Tumour weight, determined at various time-points, was found to be closely related to actual tumour weight (R = 0.99) and, in the PC-346 model, to the level of PSA in the plasma. Furthermore, the interobserver variation for TRUS was low for tumours above 50 mg. Thus, TRUS for murine prostate tumours proves to be an accurate, reproducible and sensitive method.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalLaboratory Animals
Volume36
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

prostatic neoplasms
Prostate
neoplasms
Ultrasonography
mice
ultrasonography
Tumor Burden
Neoplasms
prostate-specific antigen
Nude Mice
Prostatic Neoplasms
animal use reduction
Observer Variation
calipers
Prostate-Specific Antigen
pelvis
Pelvis
monitoring
Heterografts
endpoints

Keywords

  • Murine
  • Orthotopic prostate tumour
  • Transrectal ultrasonography
  • Validation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Kraaij, R., Van Weerden, W. M., De Ridder, C. M. A., Gussenhoven, E. J., Honkoop, J., Nasu, Y., & Bangma, C. H. (2002). Validation of transrectal ultrasonographic volumetry for orthotopic prostate tumours in mice. Laboratory Animals, 36(2), 165-172. https://doi.org/10.1258/0023677021912451

Validation of transrectal ultrasonographic volumetry for orthotopic prostate tumours in mice. / Kraaij, R.; Van Weerden, W. M.; De Ridder, C. M A; Gussenhoven, E. J.; Honkoop, J.; Nasu, Yasutomo; Bangma, C. H.

In: Laboratory Animals, Vol. 36, No. 2, 2002, p. 165-172.

Research output: Contribution to journalArticle

Kraaij, R, Van Weerden, WM, De Ridder, CMA, Gussenhoven, EJ, Honkoop, J, Nasu, Y & Bangma, CH 2002, 'Validation of transrectal ultrasonographic volumetry for orthotopic prostate tumours in mice', Laboratory Animals, vol. 36, no. 2, pp. 165-172. https://doi.org/10.1258/0023677021912451
Kraaij, R. ; Van Weerden, W. M. ; De Ridder, C. M A ; Gussenhoven, E. J. ; Honkoop, J. ; Nasu, Yasutomo ; Bangma, C. H. / Validation of transrectal ultrasonographic volumetry for orthotopic prostate tumours in mice. In: Laboratory Animals. 2002 ; Vol. 36, No. 2. pp. 165-172.
@article{e35f03d19e0644ef9073b4e13bdaf246,
title = "Validation of transrectal ultrasonographic volumetry for orthotopic prostate tumours in mice",
abstract = "Orthotopic human prostate tumour models in athymic nude mice are regarded as being most suitable for fundamental and pre-clinical research on prostate cancer. The anatomic localization of the tumour in the pelvis, however, provides little possibility for monitoring tumour growth or regression. To assess time-related changes in orthotopic tumour volume, we applied transrectal ultrasonography (TRUS) to the murine prostate. This technique has the advantages of allowing accurate monitoring of tumours during therapeutic manipulations and a reduction of animal use due to a reduction of sacrificing endpoints. To validate the TRUS method, the mouse prostate reconstitution model, RM-9, and the prostate-specific antigen (PSA) producing human prostate cancer xenograft PC-346 were used. Volumetric calliper measurements were performed with a 30 MHz ultrasound probe designed for intra-arterial use in humans. Tumour weight, determined at various time-points, was found to be closely related to actual tumour weight (R = 0.99) and, in the PC-346 model, to the level of PSA in the plasma. Furthermore, the interobserver variation for TRUS was low for tumours above 50 mg. Thus, TRUS for murine prostate tumours proves to be an accurate, reproducible and sensitive method.",
keywords = "Murine, Orthotopic prostate tumour, Transrectal ultrasonography, Validation",
author = "R. Kraaij and {Van Weerden}, {W. M.} and {De Ridder}, {C. M A} and Gussenhoven, {E. J.} and J. Honkoop and Yasutomo Nasu and Bangma, {C. H.}",
year = "2002",
doi = "10.1258/0023677021912451",
language = "English",
volume = "36",
pages = "165--172",
journal = "Laboratory Animals",
issn = "0023-6772",
publisher = "SAGE Publications Ltd",
number = "2",

}

TY - JOUR

T1 - Validation of transrectal ultrasonographic volumetry for orthotopic prostate tumours in mice

AU - Kraaij, R.

AU - Van Weerden, W. M.

AU - De Ridder, C. M A

AU - Gussenhoven, E. J.

AU - Honkoop, J.

AU - Nasu, Yasutomo

AU - Bangma, C. H.

PY - 2002

Y1 - 2002

N2 - Orthotopic human prostate tumour models in athymic nude mice are regarded as being most suitable for fundamental and pre-clinical research on prostate cancer. The anatomic localization of the tumour in the pelvis, however, provides little possibility for monitoring tumour growth or regression. To assess time-related changes in orthotopic tumour volume, we applied transrectal ultrasonography (TRUS) to the murine prostate. This technique has the advantages of allowing accurate monitoring of tumours during therapeutic manipulations and a reduction of animal use due to a reduction of sacrificing endpoints. To validate the TRUS method, the mouse prostate reconstitution model, RM-9, and the prostate-specific antigen (PSA) producing human prostate cancer xenograft PC-346 were used. Volumetric calliper measurements were performed with a 30 MHz ultrasound probe designed for intra-arterial use in humans. Tumour weight, determined at various time-points, was found to be closely related to actual tumour weight (R = 0.99) and, in the PC-346 model, to the level of PSA in the plasma. Furthermore, the interobserver variation for TRUS was low for tumours above 50 mg. Thus, TRUS for murine prostate tumours proves to be an accurate, reproducible and sensitive method.

AB - Orthotopic human prostate tumour models in athymic nude mice are regarded as being most suitable for fundamental and pre-clinical research on prostate cancer. The anatomic localization of the tumour in the pelvis, however, provides little possibility for monitoring tumour growth or regression. To assess time-related changes in orthotopic tumour volume, we applied transrectal ultrasonography (TRUS) to the murine prostate. This technique has the advantages of allowing accurate monitoring of tumours during therapeutic manipulations and a reduction of animal use due to a reduction of sacrificing endpoints. To validate the TRUS method, the mouse prostate reconstitution model, RM-9, and the prostate-specific antigen (PSA) producing human prostate cancer xenograft PC-346 were used. Volumetric calliper measurements were performed with a 30 MHz ultrasound probe designed for intra-arterial use in humans. Tumour weight, determined at various time-points, was found to be closely related to actual tumour weight (R = 0.99) and, in the PC-346 model, to the level of PSA in the plasma. Furthermore, the interobserver variation for TRUS was low for tumours above 50 mg. Thus, TRUS for murine prostate tumours proves to be an accurate, reproducible and sensitive method.

KW - Murine

KW - Orthotopic prostate tumour

KW - Transrectal ultrasonography

KW - Validation

UR - http://www.scopus.com/inward/record.url?scp=0036231252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036231252&partnerID=8YFLogxK

U2 - 10.1258/0023677021912451

DO - 10.1258/0023677021912451

M3 - Article

C2 - 11943081

AN - SCOPUS:0036231252

VL - 36

SP - 165

EP - 172

JO - Laboratory Animals

JF - Laboratory Animals

SN - 0023-6772

IS - 2

ER -