Validation of rice blast resistance genes in barley using a QTL mapping population and near-isolines

Phinyarat Kongprakhon, Alfonso Cuesta-Marcos, Patrick M. Hayes, Kelley L. Richardson, Pattama Sirithunya, Kazuhiro Sato, Brian Steffenson, Theerayuth Toojinda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

There are prior reports of Pyricularia grisea-the causal agent of blast of rice-causing disease in barley. In order to determine the specificity of this resistance in barley, we extended our previous mapping efforts to include blast isolates from barley and rice grown in Thailand and we assessed two resistance phenotypes: leaf blast (LB) and neck blast (NB). The largest-effect resistance QTL, on chromosome 1H, was associated with NB and LB and is located in a region rich in resistance genes, including QTL conferring resistance to stripe rust (incited by Puccinia striiformis f. sp. hordei) and the mildew (Blumeria graminis f. sp. hordei) resistance gene Mla. The LB, NB and mildew resistance alleles trace to one parent (Baronesse) whereas the stripe rust resistance allele traces to the other parent (BCD47) of the mapping population. Baronesse is the susceptible recurrent parent of a set of near-isogenic lines (NILs) for three stripe rust resistance QTL, including one on 1H. Unigene (EST) derived single nucleotide polymorphism haplotypes of these NILs were aligned with the blast mapping population QTL using Mla as an anchor. Baronesse and all NILs without the 1H introgression were resistant to LB and NB. However, two NILs with the 1H introgression were resistant to LB and NB. Both are resistant to stripe rust. Therefore, the QTL conferring resistance to stripe rust is separable by recombination from the blast resistance QTL.

Original languageEnglish
Pages (from-to)341-349
Number of pages9
JournalBreeding Science
Volume59
Issue number4
DOIs
Publication statusPublished - 2009

Fingerprint

blast disease
isogenic lines
Hordeum
quantitative trait loci
Neck
neck
barley
Population
Genes
genes
Pyricularia grisea
leaves
introgression
Alleles
alleles
Expressed Sequence Tags
Blumeria graminis f. sp. hordei
Thailand
Puccinia striiformis
unigenes

Keywords

  • Barley
  • Near-isolines
  • QTL mapping
  • Resistance gene
  • Rice blast

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Kongprakhon, P., Cuesta-Marcos, A., Hayes, P. M., Richardson, K. L., Sirithunya, P., Sato, K., ... Toojinda, T. (2009). Validation of rice blast resistance genes in barley using a QTL mapping population and near-isolines. Breeding Science, 59(4), 341-349. https://doi.org/10.1270/jsbbs.59.341

Validation of rice blast resistance genes in barley using a QTL mapping population and near-isolines. / Kongprakhon, Phinyarat; Cuesta-Marcos, Alfonso; Hayes, Patrick M.; Richardson, Kelley L.; Sirithunya, Pattama; Sato, Kazuhiro; Steffenson, Brian; Toojinda, Theerayuth.

In: Breeding Science, Vol. 59, No. 4, 2009, p. 341-349.

Research output: Contribution to journalArticle

Kongprakhon, P, Cuesta-Marcos, A, Hayes, PM, Richardson, KL, Sirithunya, P, Sato, K, Steffenson, B & Toojinda, T 2009, 'Validation of rice blast resistance genes in barley using a QTL mapping population and near-isolines', Breeding Science, vol. 59, no. 4, pp. 341-349. https://doi.org/10.1270/jsbbs.59.341
Kongprakhon, Phinyarat ; Cuesta-Marcos, Alfonso ; Hayes, Patrick M. ; Richardson, Kelley L. ; Sirithunya, Pattama ; Sato, Kazuhiro ; Steffenson, Brian ; Toojinda, Theerayuth. / Validation of rice blast resistance genes in barley using a QTL mapping population and near-isolines. In: Breeding Science. 2009 ; Vol. 59, No. 4. pp. 341-349.
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