Vacuolar H+-ATPase localized in plasma membranes of malaria parasite cells, Plasmodium falciparum, is involved in regional acidification of parasitized erythrocytes

Mitsuko Hayashi, Hiroshi Yamada, Toshihide Mitamura, Toshihiro Horii, Akitsugu Yamamoto, Yoshinori Moriyama

Research output: Contribution to journalArticle

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Abstract

Recent biochemical studies involving 2',7'-bis-(2-carboxyethyl)-5,6-carboxylfluorescein (BCECF)-labeled saponin-permeabilized and parasitized erythrocytes indicated that malaria parasite cells maintain the resting cytoplasmic pH at about 7.3, and treatment with vacuolar proton-pump inhibitors reduces the resting pH to 6.7, suggesting proton extrusion from the parasite cells via vacuolar H+-ATPase (Saliba, K. J., and Kirk, K. (1999) J. Biol. Chem. 274, 33213-33219). In the present study, we investigated the localization of vacuolar H+-ATPase in Plasmodium falciparum cells infecting erythrocytes. Antibodies against vacuolar H+-ATPase subunit A and B specifically immunostained the infecting parasite cells and recognized a single 67- and 55-kDa polypeptide, respectively. Immunoelectron microscopy indicated that the immunological counterpart of V-ATPase subunits A and B is localized at the plasma membrane, small clear vesicles, and food vacuoles, a lower extent being detected at the parasitophorus vacuolar membrane of the parasite cells. We measured the cytoplasmic pH of both infected erythrocytes and invading malaria parasite cells by microfluorimetry using BCECF fluorescence. It was found that a restricted area of the erythrocyte cytoplasm near a parasite cell is slightly acidic, being about pH 6.9. The pH increased to pH 7.3 upon the addition of either concanamycin B or bafilomycin A1, specific inhibitors of vacuolar H+-ATPase. Simultaneously, the cytoplasmic pH of the infecting parasite cell decreased from pH 7.3 to 7.1. Neither vanadate at 0.5 mM, an inhibitor of P-type H+-ATPase, nor ethylisopropylamiloride at 0.2 mM, an inhibitor of Na+/H+-exchanger, affected the cytoplasmic pH of erythrocytes or infecting parasite cells. These results constitute direct evidence that plasma membrane vacuolar H+-ATPase is responsible for active extrusion of protons from the parasite cells.

Original languageEnglish
Pages (from-to)34353-34358
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number44
Publication statusPublished - Nov 3 2000

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Vacuolar Proton-Translocating ATPases
Acidification
Cell membranes
Plasmodium falciparum
Malaria
Parasites
Erythrocytes
Cell Membrane
Extrusion
Protons
Cytophotometry
Sodium-Hydrogen Antiporter
Proton-Translocating ATPases
Vanadates
Immunoelectron Microscopy
Proton Pump Inhibitors
Saponins
Vacuoles
Adenosine Triphosphatases
Microscopic examination

ASJC Scopus subject areas

  • Biochemistry

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Vacuolar H+-ATPase localized in plasma membranes of malaria parasite cells, Plasmodium falciparum, is involved in regional acidification of parasitized erythrocytes. / Hayashi, Mitsuko; Yamada, Hiroshi; Mitamura, Toshihide; Horii, Toshihiro; Yamamoto, Akitsugu; Moriyama, Yoshinori.

In: Journal of Biological Chemistry, Vol. 275, No. 44, 03.11.2000, p. 34353-34358.

Research output: Contribution to journalArticle

Hayashi, Mitsuko ; Yamada, Hiroshi ; Mitamura, Toshihide ; Horii, Toshihiro ; Yamamoto, Akitsugu ; Moriyama, Yoshinori. / Vacuolar H+-ATPase localized in plasma membranes of malaria parasite cells, Plasmodium falciparum, is involved in regional acidification of parasitized erythrocytes. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 44. pp. 34353-34358.
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