Vacuolar citrate/H+ symporter of citrus juice cells

Takehiko Shimada, Ryohei Nakano, Vladimir Shulaev, Avi Sadka, Eduardo Blumwald

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We have isolated a cDNA, designated Citrus sinensis citrate transporter 1 CsCit1 encoding a novel vacuolar citrate/symporter. Immunoblots using antibodies raised against CsCit1 showed that the protein is localized to the juice sac cell vacuoles. The highest expression of CsCit1 and the amount of protein in the juice sac cell vacuoles coincided with the developmental stage at which the vacuolar citrate content began declining with the concomitant increase in vacuolar pH. Vacuoles from Sacharomyces cereviseae expressing CsCit1 displayed a citrate-dependent H+ efflux, and our results clearly demonstrate that CsCit1 is able to mediate the electroneutral co-transport of H+ and citrate ions, since the citrate-dependent H+ fluxes are not affected by changing the electrical potential difference across the tonoplast. The roles of CsCit1 in mediating citrate efflux from the vacuole and on citric acid homoestasis in Citrus juice sac cells are discussed.

Original languageEnglish
Pages (from-to)472-480
Number of pages9
JournalPlanta
Volume224
Issue number2
DOIs
Publication statusPublished - Jul 2006
Externally publishedYes

Fingerprint

symporters
Symporters
citrus juices
Citrus
Citric Acid
citrates
Vacuoles
vacuoles
cells
juices
tonoplast
Citrus sinensis
citric acid
transporters
Proteins
proteins
Complementary DNA
developmental stages
Ions
ions

Keywords

  • Acidity
  • Citrate
  • Citrus
  • Homeostasis
  • Symporter
  • Vacuoles

ASJC Scopus subject areas

  • Plant Science

Cite this

Shimada, T., Nakano, R., Shulaev, V., Sadka, A., & Blumwald, E. (2006). Vacuolar citrate/H+ symporter of citrus juice cells. Planta, 224(2), 472-480. https://doi.org/10.1007/s00425-006-0223-2

Vacuolar citrate/H+ symporter of citrus juice cells. / Shimada, Takehiko; Nakano, Ryohei; Shulaev, Vladimir; Sadka, Avi; Blumwald, Eduardo.

In: Planta, Vol. 224, No. 2, 07.2006, p. 472-480.

Research output: Contribution to journalArticle

Shimada, T, Nakano, R, Shulaev, V, Sadka, A & Blumwald, E 2006, 'Vacuolar citrate/H+ symporter of citrus juice cells', Planta, vol. 224, no. 2, pp. 472-480. https://doi.org/10.1007/s00425-006-0223-2
Shimada T, Nakano R, Shulaev V, Sadka A, Blumwald E. Vacuolar citrate/H+ symporter of citrus juice cells. Planta. 2006 Jul;224(2):472-480. https://doi.org/10.1007/s00425-006-0223-2
Shimada, Takehiko ; Nakano, Ryohei ; Shulaev, Vladimir ; Sadka, Avi ; Blumwald, Eduardo. / Vacuolar citrate/H+ symporter of citrus juice cells. In: Planta. 2006 ; Vol. 224, No. 2. pp. 472-480.
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